Research of low molecular fraction of C. albicans fungus cells by the ELISA in subcutaneous administration

Abstract

The purpose of this work is a study of low molecular weight fraction with C. albicans protein concentrations of 1, 2, 3, and 4 mg/ml by antibody titers in subcutaneous administration. A low molecular fraction of C. albicans proteins in concentration 1, 2, 3, 4, and 5 mg/ml has been studied in white mice. Mice have subcutaneously injected 0.2 ml of the test fraction into the upper part of the right hind paw. After 14 days, 0.2 ml of test fractions were injected repeatedly into the upper part of the left hind paw. 1 month for one group and 3 months for the second group of experimental animals after the second injection intraperitoneal infection of the animals was carried out. After 14 days, determination of the protective functions of the animal body by titer of C. albicans specific antibodies has been performed by enzyme-linked immunoassay. Studies have shown that the low molecular weight fraction of C. albicans fungi cells antigens at the double subcutaneous injection of 0.2 ml does not provide the activation of immune mechanisms. The low molecular weight fraction of C. albicans proteins at subcutaneous administration does not activate the body's defense mechanisms in preventing candidiasis.