Research of Biological Properties of Enterovirus
Strains Associated With Ischemic Stroke

Andriushkova, Natalia G; Shyrobokov, V. P.; Turchyna, Nataliia S; Melnyk, Volodymyr; Kuzminska, Olena V; Dolinchuk, L.V.

doi:10.36740/wlek202003102

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…They are around 30 nm in diameter and have a single-stranded RNA genome of approximately 7500 base pairs. The symptoms caused by enterovirus infections are usually mild, but they have also been associated with more serious diseases like type 1 diabetes, heart attack and acute stroke [ 1 , 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Antiviral Mechanisms of N-Phenyl Benzamides on Coxsackie Virus A9

et al. 2023

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Enteroviruses are one of the most abundant groups of viruses infecting humans, and yet there are no approved antivirals against them. To find effective antiviral compounds against enterovirus B group viruses, an in-house chemical library was screened. The most effective compounds against Coxsackieviruses B3 (CVB3) and A9 (CVA9) were CL212 and CL213, two N-phenyl benzamides. Both compounds were more effective against CVA9 and CL213 gave a better EC50 value of 1 µM with high a specificity index of 140. Both drugs were most effective when incubated directly with viruses suggesting that they mainly bound to the virions. A real-time uncoating assay showed that the compounds stabilized the virions and radioactive sucrose gradient as well as TEM confirmed that the viruses stayed intact. A docking assay, taking into account larger areas around the 2-and 3-fold axes of CVA9 and CVB3, suggested that the hydrophobic pocket gives the strongest binding to CVA9 but revealed another binding site around the 3-fold axis which could contribute to the binding of the compounds. Together, our data support a direct antiviral mechanism against the virus capsid and suggest that the compounds bind to the hydrophobic pocket and 3-fold axis area resulting in the stabilization of the virion.

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Section: Introductionmentioning

confidence: 99%

Antiviral Mechanisms of N-Phenyl Benzamides on Coxsackie Virus A9

et al. 2023

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The Role of Enteroviruses in the Development of Ischemic Stroke and Its Outcomes

et al. 2022

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The aim: To study the role of enteroviruses (EV) in the development of ischemic stroke and its outcome. Materials and methods: The main group (MG) included 72 patients with acute cerebrovascular disorders were examined using the National Institutes of Health Stroke Scale and Barthel Index. The comparison group (CG) included 35 patients without cerebrovascular disease. Viruses were isolated from patients’ sera and identified in neutralization test. EV genomes were detected in polymerase chain reaction (PCR). Serological diagnosis was performed by enzyme-linked immunosorbent assay. Results: EV genomes were more frequently detected in the patients’ sera in MG than in CG (23.6 ± 5.9% and 2.9 ± 2.8%, p <0.05). The greater level of neurological deficits was in patients with positive PCR test results comparatively with patients with negative PCR test results (11.76 ± 0.31 and 10.97 ± 0, 27, p = 0.040). The regression of neurological deficit during the treatment was a worse in patients with positive PCR test results and presence of specific IgG compared with patients with positive PCR test results and absence of specific IgG (11.2 ± 2.6% and 19.6 ± 2.4%, p = 0.031). Conclusions: The trigger role of EV in the development of IS is established. PCR is recommended for diagnosis of EV in patients with IS.

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Research of Biological Properties of Enterovirus Strains Associated With Ischemic Stroke

Cited by 2 publications

References 3 publications

Antiviral Mechanisms of N-Phenyl Benzamides on Coxsackie Virus A9

Antiviral Mechanisms of N-Phenyl Benzamides on Coxsackie Virus A9

The Role of Enteroviruses in the Development of Ischemic Stroke and Its Outcomes

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