Research Note: Prevalence and zoonotic concern of Blastocystis in farmed chickens in southern China

Liu, Xuehan; Ni, Fuzhen; Li, Junqiang; Wang, Rongjun; Yang, Xuefeng; Ge, Yaming; Zhang, Longxian; Qi, Meng

doi:10.1016/j.psj.2022.102182

Cited by 4 publications

(4 citation statements)

References 12 publications

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“…Blastocystis was detected in 13% (6/46) and 2.1% (3/140) of samples from Heilongjiang Province in China, which were lower than the overall infection rate of Blastocystis in Hotan Black chickens (26.3%, 162/617) detected here (Chen et al, 2023;Wang et al, 2018). In another study, Liu et al (2022) reported that the positive rate of this protozoan was 20.1% (201/1, 000). To date, the overall infection rate of Blastocystis in chickens is 30.4% (1193/3, 927) globally and 20.1% (372/1, 854) in China according to previous studies and this study (Table 4).…”

Section: Discussionmentioning

confidence: 83%

“…In another study, Liu et al. (2022) reported that the positive rate of this protozoan was 20.1% (201/1, 000). To date, the overall infection rate of Blastocystis in chickens is 30.4% (1193/3, 927) globally and 20.1% (372/1, 854) in China according to previous studies and this study (Table 4).…”

Section: Discussionmentioning

confidence: 92%

“…The following 10 subtypes of Blastocystis have been detected in chickens: ST1, ST2, ST5–ST8, ST10, ST14, ST25, and ST29, with ST6 and ST7 being the most common subtypes (Hublin et al., 2021; Maloney et al., 2021; Mohd Zain et al., 2018). To the best of our knowledge, few studies have been published involving Blastocystis in chickens in China, which showed a total prevalence of 9.2% (209/1186), and ST6 and ST7 are the only two subtypes that have been reported in chickens (Chen et al., 2023; Liu et al., 2022; Wang et al., 2018).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of Blastocystis spp. in Hotan Black chickens in southern Xinjiang

Feng,

Xin,

Zhang

et al. 2023

J Eukaryotic Microbiology

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To determine the infection status and assess the zoonotic potential of Blastocystis spp. in Hotan Black chickens in southern Xinjiang, China, fecal samples were collected from 617 chickens on 18 large‐scale farms. The presence of Blastocystis spp. was determined using polymerase chain reaction based on the small subunit rRNA (SSU rRNA) locus. The results revealed an overall infection rate of 26.3% (162/617). Samples from Farm 1 in Luopu County showed the highest infection rate (76.3%, 29/38). The highest and lowest infection rates were detected in the <30‐day (34.4%, 43/125) and > 90‐day age groups (12.4%, 11/89), respectively. The infection rate decreased with increasing age. Statistical analysis showed significant differences in the infection rates of Blastocystis spp. among the different sampling sites (p < 0.05) and age groups (p < 0.05). Four Blastocystis spp. subtypes (ST6, ST7, ST10, and ST23) were identified. The infection rates of the zoonotic subtypes, ST6 and ST7, were 3.2% (20/617) and 22.2% (137/617), respectively. The presence of Blastocystis spp. and zoonotic subtypes provided evidence for the potential transmission of this pathogen between Hotan Black chickens and humans, especially in animal handlers in this area.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of Blastocystis spp. in Hotan Black chickens in southern Xinjiang

Feng,

Xin,

Zhang

et al. 2023

J Eukaryotic Microbiology

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“…The five known Blastocystis subtypes (ST1 to ST4 and ST7) identified in this study are also prevalent in various animals in China [ 18 ]: ST1 in non-human primates, dogs, foxes, goats, sheep, pigs, cattle, civets, bears, and birds; ST2 in non-human primates, bears, and some captive wild animals; ST3 in non-human primates, goats, sheep, cattle, pigs, raccoon dogs, and rex rabbits; ST4 in rodents, bears, and whooper swans; and ST7 in chickens, whooper swans, and some birds [ 7 , 14 , 15 , 17 , 29 , 31 , 35 – 37 , 40 , 41 ]. These findings suggest the potential for zoonotic transmission of Blastocystis .…”

Section: Discussionmentioning

confidence: 99%

Molecular prevalence and subtype distribution of Blastocystis spp. among children who have diarrheia or are asymptomatic in Wenzhou, Zhejiang Province, China

Zhao,

Ren,

Wang

et al. 2024

Parasite

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Blastocystis sp., a significant zoonotic parasite with a global distribution, was the focus of this study, which aimed to investigate its prevalence and genetic diversity among diarrheic and asymptomatic children in Wenzhou, China. We collected 1,032 fecal samples from Yuying Children’s Hospital, Wenzhou, China, comprising 684 from children with diarrhea and 348 from asymptomatic children. Genomic DNA extracted from these samples was used to detect Blastocystis spp. by PCR, targeting the small subunit ribosomal RNA gene. Subsequently, a phylogenetic tree was constructed, applying the maximum likelihood method. Blastocystis spp. were detected in 67 (6.5%) of the fecal samples. The prevalence rate of Blastocystis spp. in diarrheic children (8.8%; 60/684) was significantly higher than that in asymptomatic children (2.0%; 7/348) (χ 2 = 17.3, p < 0.001). Sequence analysis of the SSU rRNA gene identified five known Blastocystis spp. subtypes, ST1 (n = 12), ST2 (n = 5), ST3 (n = 35), ST4 (n = 12), and ST7 (n = 3). ST1 and ST3 were present in both diarrheic and asymptomatic children, while ST2, ST4, and ST7 were exclusive to diarrheic children. Intra-subtype genetic polymorphisms were identified, comprising four variations in ST1 (ST1-1 to ST1-4), five in ST3 (ST3-1 to ST3-5), two in ST4 (ST4-1 and ST4-2), and two in ST7 (ST7-1 and ST7-2). Notably, ST1-2 to ST1-4, ST3-3 to ST3-5, and ST7-1 and ST7-2 represent newly identified variations. The composition and genetic characteristics of subtypes among children in this region suggest various sources of infection, including human-to-human and animal-to-human transmission.

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First identification and molecular subtyping of Blastocystis in free-living wild birds from urban Xinxiang, China

Feng

Zheng

et al. 2023

Vet Res Commun

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Research Note: Prevalence and zoonotic concern of Blastocystis in farmed chickens in southern China

Cited by 4 publications

References 12 publications

Molecular characterization of Blastocystis spp. in Hotan Black chickens in southern Xinjiang

Molecular characterization of Blastocystis spp. in Hotan Black chickens in southern Xinjiang

Molecular prevalence and subtype distribution of Blastocystis spp. among children who have diarrheia or are asymptomatic in Wenzhou, Zhejiang Province, China

First identification and molecular subtyping of Blastocystis in free-living wild birds from urban Xinxiang, China

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