“…Nevertheless, it is a fast, easy, and low-cost technique that may provide useful information and has, therefore, been used by several researchers to evaluate immunoreactivity of IgYs[ 32 , 34 , 36 , 39 , 76 ]. In most cases, however, evaluation of IgY immunoreactivity involves determination of titer against the target antigen through non-competitive ELISAs (indicative references[ 32 , 35 , 45 , 76 , 78 ]). Moreover, other immunochemical characteristics of the isolated IgYs are assessed, such as putative cross-reactivity with various substances through competitive ELISAs (indicative references[ 31 , 36 , 39 ]).…”