Research into Morphofunctional Characteristics of Erythrocytes in COVID-19 Patients

Abstract

In this paper, the erythrocytes of healthy donors and people with a confirmed diagnosis of COVID-19 were tested by Raman spectroscopy and laser interference microscopy. We argue that erythrocytes (red blood cells) in COVID-19 patients have an irregular shape, and their morphometric characteristics are impaired in comparison to healthy red blood cells. Raman spectroscopy also allows us to detect a decreased oxygen transport function of these erythrocytes. The combination of these methods—Raman spectroscopy and laser interference microscopy—is a highly effective method for the diagnosis of SARS-CoV-2.

Erythrocytes Functionality in SARS-CoV-2 Infection: Potential Link with Alzheimer’s Disease

“…For example, in some COVID-19 patients, the appearance of rigid RBCs, including mushroom-shaped RBCs [ 203 ], schizocytes [ 204 ], and sphero-echinocytes with irreversible metabolic, structural changes [ 205 ] and phosphatidylserine inversion [ 206 ] in the bloodstream points to an alteration in membrane charge [ 207 ], structural membrane damage [ 20 ], and decreased turnover rate [ 208 ]. Altogether, this can dramatically increase aggregation [ 209 ] and reduce microvascular perfusion [ 210 , 211 ], eventually affecting the efficiency of oxygen transport [ 212 , 213 ]. Therefore, the analysis of changes in the morphology and appearance of rigid cells in the bloodstream of the COVID-19 patient can enrich knowledge of the progression of impaired tissue perfusion.…”

Rapid, label-free classification of glioblastoma differentiation status combining confocal Raman spectroscopy and machine learning

“…Ascending numbers of studies successfully follow this approach using either small specimen counts but also proving its ability on data retrieved from whole tissue sections. 30–32…”

“…Ascending numbers of studies successfully follow this approach using either small specimen counts but also proving its ability on data retrieved from whole tissue sections. [30][31][32] Here we evaluate a machine learning-assisted classification approach to identify GSCs using a collection of recently described in vitro models, that resemble various different neuropathological relevant, genetic markers of primary tumors. 33 Especially heterogenetic GSCs remain underestimated in clinical diagnostic routines due to missing evidence and methodology.…”

Raman and fourier transform infrared spectroscopy techniques for detection of coronavirus (COVID-19): a mini review

“…The study focused on the fact that the COVID-19 serum samples had an increase in blood lipids, nitrogen compounds and nucleic acids, while content of protein and tryptophan decreased, which also illustrated Raman spectroscopy as a rapid and economic detection platform. Since hematological disease is a common symptom of COVID-19 ( Bergamaschi et al, 2021 ), ( Revin et al, 2022 ) combined Raman spectroscopy and laser interference microscopy techniques to specifically identify decreased erythrocyte oxygen transport function and abnormal appearance in patients with confirmed COVID-19 compared with healthy people. In addition to blood tests, other body fluids like saliva can also get more accurate results.…”