Research for the effect of octylphenol on spermatogenesis and proteomic analysis in octylphenol-treated mice testes

Abstract

OP (octylphenol), an environmental oestrogen was administered, and differentially expressed proteins were analysed in mice testes to clarify its mechanism of action in male sterility. Male Kunming suckling mice (10 days old) were subcutaneously injected with OP at a dose of 10 μg/kg per day, 50 μg/kg per day and 100 μg/kg per day as low-, medium- and high-dose groups, respectively, for 35 days. Animals in the control group received subcutaneous injections of olive oil at a dose of 10 μl/mouse per day. Serum oestradiol, testosterone, FSH (follicle stimulating hormone) and LH (luteinizing hormone) levels were measured on day 45. The left testes were removed for tissue analysis, and the right testes were analysed for differentially expressed proteins by using two-dimensional gel electrophoresis and MS. Tissue analysis showed that mice spermatogenesis was blocked at the round spermatid stage in the high-dose group, whereas no such changes were found in the medium- and low-dose groups. Higher serum oestradiol (P<0.05) and lower testosterone (P<0.05) levels were found in the medium- and high-dose groups. There was no significant difference in serum oestradiol and testosterone levels in the low-dose and control groups. No significant influence of OP was seen on serum FSH and LH levels in all OP-treated animals. The results from four differentially expressed proteins such as PPIA (peptidyl-prolyl cis-trans isomerase A), PEBP1 (phosphatidylethanolamine-binding protein1), TPI (triose-phosphate isomerase) and TCP-1 (T-complex protein 1) in the high-dose and control groups showed up-regulation of PPIA expression and down-regulation in PEBP1, TPI and TCP-1 expressions. These findings will contribute to clarify the mechanism of male sterility by environmental oestrogens.

Chronic exercise training attenuates prostate cancer-induced molecular remodelling in the testis

“…Increased levels of glucose in the testis of sedentary PCa animals suggests a reduction in glucose utilization which, based also on the decreased levels of Tpi1 in germ cells of PCa animals, may reflect a general impairment of glycolysis. Concordantly, deregulation of glycolytic enzymes, including Tpi1, has been associated by others with impaired spermatogenesis and germ cell apoptosis [56]. In an attempt to compensate for decreased glucose metabolism, Sertoli cells appeared to increase the metabolization of ketone bodies, particularly β-hydroxybutyrate, as an alternative energy source, leading to decreased testis levels.…”

In Utero Exposure to Environmentally Relevant Concentrations of PCB 153 and PCB 118 Disrupts Fetal Testis Development in Sheep

“…A downregulation of PPIA and TCP-1 was reported in mouse testis after treatment with octylphenol (Li et al, 2011). Proteasome subunit p40 (PSMD7), a subunit of eukaryotic translation initiation factor 3 (eiF3), was upregulated for H-153/L-118, but unchanged for H-118/L-153.…”

Regulatory decisions on endocrine disrupting chemicals should be based on the principles of endocrinology

“…Our WoE analyses included the effects of atrazine on sexual differentiation in male amphibians, the effects of BPA on the prostate and mammary gland, the effects of dioxin on spermatogenesis, and the effects of perchlorate on thyroid hormone levels in humans [2]. Yet it should be noted that there were several examples we identified but did not analyze in detail, including the effects of PCB mixtures on brain sexual dimorphisms [6–8], the effects of DDT on neurobehaviors [9–15], the effects of nicotine on human male genital tract malformations [16–19], the effects of octylphenol on the testes [20–23], and the effects of tributyltin on obesity [24–27], among others.…”