Profit is the driving force for any enterprises to thrive well, because it encourages more investment into an enterprise. One of the major barriers to investing in plantain value chain is inadequate information on return to investment. Thus, this paper investigated profitability of investors along plantain value chain in Osun state. A multistage sampling procedure was used to elicit information from 100 respondents for the study. Data were analysed using descriptive analysis, budgetary analysis, and multiple regression analysis. Descriptive statistics reveal that average age was 52.2 (±11.19) years for plantain farmers, 41.8 (±10.78) years for processors and marketers 33.42 (±11.99). While, average farming experience was 26.9 (±10.88) years for plantain farmers, 12.47 (±10.78) years for processors and marketers 5.84 (±19.12). About 6.7% farmers, 15.6% processors and 13.2% marketers had access to credit facilities. The budgetary analysis showed that benefit-cost ratios were $1.38, $1.30 and $1.19 for the farmers, processors and marketers, respectively. Multiple regression estimates revealed that insecticide used (p<0.1) and numbers of plantain harvested (p<0.01) significantly influenced the profitability of the plantain farmers, while age (p<0.1), level of formal education (p<0.05), amount invested into the business (p<0.01) and household size (p<0.05) significantly influenced the profitability of the plantain marketers. Only household size (p<0.01) significantly influenced the profitability of the plantain processor. In accordance with the findings of the study, we therefore recommend that subsidized cost of inputs and better access to credit among the investors along the value chain would increase the level of return to the investment.