Research Article Molecular mapping and candidate gene analysis for resistance to powdery mildew in Cucumis sativus stem.

Liu, P.N.; Han, Moonsik; Lu, Hongwei; Cui, J.Y.; Tian, Guobing; Wehner, Todd C.; Gu, Xingfang; Zhang, S.P.

doi:10.4238/gmr16039680

Cited by 17 publications

(12 citation statements)

References 20 publications

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“…The inheritance of PM resistance is complicated in cucumber. Few studies reported that PM resistance was controlled by a recessive gene [ 9 , 10 ]. Most studies showed that PM resistance was a quantitative trait that controlled by multiple genes and likely recessively inherited in cucumber [ 11 , 12 , 16 , 17 , 18 , 19 , 21 , 25 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…In cucumber, QTLs for PM resistance have previously been detected on chromosome 5 in an interval where the CsaMLO8 gene is located [ 10 , 11 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 24 , 25 ]. In the CG population, the QTL pmG5.4 is colocalized with CsaMLO8 , and the CG lines carrying the mutant allele (with TE insertion) of CsaMLO8 [ 28 ] showed a significant reduction of disease symptoms.…”

Section: Discussionmentioning

confidence: 99%

“…In 2006, the first quantitative trait locus (QTL) mapping was carried out to study the PM resistance in accession PI 197,088 (from India and highly resistant) and cv. Santou (from Japan, partial resistant) by using a set of recombinant inbred lines (RILs) [ 9 , 10 ]. This was followed by many other studies resulting in many detected QTLs for PM resistance that are scattered over all the seven cucumber chromosomes [ 3 , 11 , 12 , 13 , 14 , 15 ] ( Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

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Identification of Novel Loci and Candidate Genes for Resistance to Powdery Mildew in a Resequenced Cucumber Germplasm

et al. 2021

Genes

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Powdery mildew (PM) is one of the most serious diseases in cucumber and causes huge yield loss. Multiple quantitative trait loci (QTLs) for PM resistance have been reported in previous studies using a limited number of cucumber accessions. In this study, a cucumber core germplasm (CG) consisting of 94 resequenced lines was evaluated for PM resistance in four trials across three years (2013, 2014, and 2016). These trials were performed on adult plants in the field with natural infection. Using genome-wide association study (GWAS), 13 loci (pmG1.1, pmG1.2, pmG2.1, pmG2.2, pmG3.1, pmG4.1, pmG4.2, pmG5.1, pmG5.2, pmG5.3, pmG5.4, pmG6.1, and pmG6.2) associated with PM resistance were detected on all chromosomes except for Chr.7. Among these loci, ten were mapped to chromosomal intervals where QTLs had been reported in previous studies, while, three (pmG2.1, pmG3.1, and pmG4.1) were novel. The loci of pmG2.1, pmG5.2, pmG5.3 showed stronger signal in four trials. Based on the annotation of homologous genes in Arabidopsis and pairwise LD correlation analysis, candidate genes located in the QTL intervals were predicted. SNPs in these candidate genes were analyzed between haplotypes of highly resistant (HR) and susceptible (HS) CG lines, which were defined based on combing disease index data of all trials. Furthermore, candidate genes (Csa5G622830 and CsGy5G015660) reported in previous studies for PM resistance and cucumber orthologues of several PM susceptibility (S) genes (PMR5, PMR-6, and MLO) that are colocalized with certain QTLs, were analyzed for their potential contribution to the QTL effect on both PM and DM in the CG population. This study shows that the CG germplasm is a very valuable resource carrying known and novel QTLs for both PM and DM resistance, which can be exploited in cucumber breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of Novel Loci and Candidate Genes for Resistance to Powdery Mildew in a Resequenced Cucumber Germplasm

et al. 2021

Genes

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“…A single recessive gene pm for PM resistance in leaves has been mapped to an approximately 468 kb region on chromosome 5 in IL52 ( Zhang et al, 2018 ). The resistance to PM in the stem of NCG-12 is also controlled by a single recessive nuclear gene ( pm-s ) ( Liu et al, 2017 ). The recessive inheritance of PM is not convenient to use in cucumber breeding ( Xu X. et al, 2016 ).…”

Section: Inheritance Quantitative Trait Loci Mapping and Candidate Ge...mentioning

confidence: 99%

Research Advances in Genetic Mechanisms of Major Cucumber Diseases Resistance

Wei

Yan

et al. 2022

Front. Plant Sci.

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Cucumber (Cucumis sativus L.) is an important economic vegetable crop worldwide that is susceptible to various common pathogens, including powdery mildew (PM), downy mildew (DM), and Fusarium wilt (FM). In cucumber breeding programs, identifying disease resistance and related molecular markers is generally a top priority. PM, DM, and FW are the major diseases of cucumber in China that cause severe yield losses and the genetic-based cucumber resistance against these diseases has been developed over the last decade. Still, the molecular mechanisms of cucumber disease resistance remain unclear. In this review, we summarize recent findings on the inheritance, molecular markers, and quantitative trait locus mapping of cucumber PM, DM, and FM resistance. In addition, several candidate genes, such as PM, DM, and FM resistance genes, with or without functional verification are reviewed. The data help to reveal the molecular mechanisms of cucumber disease resistance and provide exciting new opportunities for further resistance breeding.

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“…Among the QTLs, the QTL pm 1.1 and the pm 5.2 are effective in leaf and hypocotyl resistance [20]. Some candidate genes of powdery mildew resistance were suggested, which included the MLO-like gene [21,23], Csa1M064780, Csa1M064790 [24], the CsSGR gene [25], the cyclin-like (cyclin L) gene, genes encoding the F-box protein, kinesin protein, calcium transporting ATPase, Ras-related protein, and lysine-specific histone demethylase 1 (LSD1) [15]. Interestingly, the powdery mildew resistance is reported to be closely linked to the downy mildew resistance [16,22].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Genetic Diversity of the Breeding Lines and a Genome Wide Association Study of Three Horticultural Traits Using Worldwide Cucumber (Cucumis spp.) Germplasm Collection

et al. 2020

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Cucumbers are an important economic vegetable crop that is used for fresh or processing purposes worldwide. In this study, we used 264 accessions that consisted of world-wide wild germplasms and advanced breeding lines in order to understand the genetic diversity and the genetic correlation among the germplasm collection. A genotyping-by-sequencing (GBS) approach was applied to obtain dense genome-wide markers coverage (>12,082 SNPs) to construct a high-density haplotype map. Various population stratification methods were performed, and three subgroups were divided based on the genetic diversity, which reflected their geographic regions. According to the phylogenetic analysis, the breeding lines were separated from wild germplasms, and the two distinct groups were divided within the breeding lines. One of the groups mainly consisted of East-Asian varieties, which showed the unique homogenous genotype patterns. Using this germplasm collection, three important horticultural traits of cucumbers—powdery mildew resistance, spine color, and fruit stalk-end color—were evaluated and used to conduct the genome-wide association study (GWAS). All of the significant SNPs and two novel candidate genes (Csa5G453160 and Csa5G471070) for the powdery mildew were identified in chromosome 5 from the natural population, which is where reported major QTLs from various bi-parental population are located. Furthermore, two candidate genes, Csa1G006300 and Csa3G824850, and four candidate genes, Csa2G368270, Csa3G236570, Csa5G175680, and Csa6G448170, were identified for the spine color and the fruit stalk-end color, respectively. These results are expected to be helpful to develop molecular markers of the horticultural traits in cucumbers.

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Research Article Molecular mapping and candidate gene analysis for resistance to powdery mildew in Cucumis sativus stem.

Cited by 17 publications

References 20 publications

Identification of Novel Loci and Candidate Genes for Resistance to Powdery Mildew in a Resequenced Cucumber Germplasm

Identification of Novel Loci and Candidate Genes for Resistance to Powdery Mildew in a Resequenced Cucumber Germplasm

Research Advances in Genetic Mechanisms of Major Cucumber Diseases Resistance

Assessment of the Genetic Diversity of the Breeding Lines and a Genome Wide Association Study of Three Horticultural Traits Using Worldwide Cucumber (Cucumis spp.) Germplasm Collection

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