Research Article Isolation, diversity, and biotechnological potential of maize (&lt;i&gt;Zea&lt;/i&gt; &lt;i&gt;mays&lt;/i&gt;) grains bacteria

Santos, Fabiane Cristina dos; Castro, F.F. de; Apolonio, T.M.; Yoshida, Luciana; Martim, Damaris Batistão; Tessmann, Dauri José; Barbosa-Tessmann, Ione Parra

doi:10.4238/gmr18320

Cited by 3 publications

(1 citation statement)

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“…Firmicutes are Gram-positive bacteria with a low G + C content in their genomes and degrade macromolecular compounds such as proteins, starch, and cellulose (Zeng et al, 2020 ). Proteobacteria are the largest bacterial phylum, including not only Erwiniaceae, Sphingomonas Paucimobilis, Methylobacterium , and Pseudomonas adaceae , but also pathogenic bacteria such as Escherichia Coli, Vibrio , and Spirillum (Santos et al, 2019 ). Proteobacteria play an important role in the degradation of organic matter and the carbon-nitrogen cycles during anaerobic digestion (Zhao et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of Lactobacillus plantarum (L) and molasses (M) on nutrient composition, aerobic stability, and microflora of alfalfa silage in sandy grasslands

Peng,

Zhang,

Wei

et al. 2024

Front. Microbiol.

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The objective of this experiment was to investigate the effects of Lactobacillus plantarum and molasses on the nutrient composition, fermentation quality, bacterial count, aerobic stability, and microflora of alfalfa silage in sandy grasslands. The experimental treatments included control (CK), 106 CFU/g Lactobacillus plantarum (L), 5% molasses (M), and 106 CFU/g Lactobacillus plantarum + 5% molasses (LM). The nutrient composition, fermentation quality, bacterial count, aerobic stability, and microflora were determined after 14 days and 56 days of ensiling, respectively. The results showed that the addition of L, M, and LM reduced dry matter loss (DM), neutral detergent fiber (NDF), and acid detergent fiber (ADF) content, and increased water-soluble carbohydrates (WSC) and ether extract (EE) content, compared to the CK group. Meanwhile, more lactic acid (LA) and accelerated fermentation were observed, causing the pH value to drop below 4.5 in the L, M, and LM groups after 56 days of ensiling. The addition of L, M, and LM promoted lactic acid bacteria (LAB), and inhibited yeast. The addition of L significantly increased the content of acetic acid (AA). In terms of microflora, the addition of L, M, and LM made Firmicutes become the dominant bacterial phylum earlier, while Lactobacillus, Weissella, and Pediococcus had a higher abundance. According to the result of Pearson's correlation, there is a very significant negative correlation between pH value and Lactobacillus (P < 0.01) and a very significant positive correlation between pH value and Lactococcus, Enterobacter, Enterococcus, and Leuconostoc (P < 0.01), which may be inhibited by Lactobacillus under the decreased pH value. The results of the prediction of microbial genes indicated that the addition of M could enhance the carbohydrate metabolism and membrane transport metabolism, which may contribute to LA production by LAB metabolism. In general, L, M and LM all improved the fermentation quality and reduced the loss of nutrients to varying degrees, but considering the fermentation quality, the overall effects of M and LM were better than L. M and LM are recommended to be used as silage additives in the process of alfalfa silage in sandy grasslands to improve the quality.

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Section: Discussionmentioning

confidence: 99%