Research Article Gene expression analysis by RNA-sequencing of <i>Longissimus</i> <i>dorsi</i> muscle of pigs fed diets with differing lipid contents

Abstract

©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (4): gmr18307 M.C. Montenegro et al. 2 T15, respectively. Three male piglets of the Uruguayan creole breed Pampa Rocha were used per treatment, which lasted from weaning at 42 days until 77 days of age. The animals were reared in confinement on deep bedding and were slaughtered at the end of the experiment, when muscle samples were collected. Intramuscular fat content (IMF) and fatty acid composition were analyzed to determine if diets had a phenotypic effect. Gene expression analysis was performed with RNA-seq methodology to carrying out a functional analysis of genes with differential expression between treatments. The added fat to the diet did not affect IMF or fatty acid composition. However, we identified 359 genes with differential expression between treatments. These genes participate in various metabolic pathways, some of them affecting meat quality. The most relevant genes identified in this regard were PDK4 (up-regulated with T15), which is associated with energy metabolism, FOS, ATF3, MYOD1 and MAFF (all downregulated with T15), which are associated with skeletal muscle growth, and TNC (up-regulated with T15), which is associated with extracellular matrix-receptor interactions. This study of the skeletal muscle transcriptome in pigs can help understand the genetic basis of how diet affects important production traits.

Experimental Model to Substantiate the Application of New Biologically Active Substances for Correcting Physiological Processes and Functions

“…The results obtained substantiate the possibility of regulating metabolic processes by activating signaling pathways associated with cell proliferation. В последние годы большой интерес у исследователей вызывает изучение молекул, воздействующих на метаболические звенья и которые могут активировать сигнальные пути, связанные с пролиферацией, ростом и развитием [2,6].…”