Research Article First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (açaí) in cultured human cells.

Marques, E. S.; Tsuboy, Marcela Stefanini Ferreira; Carvalho, José Carlos Tavares; Rosa, Paulo; Perazzo, Fábio Ferreira; Gaivão, Isabel; Maistro, Edson Luis

doi:10.4238/gmr16039700

Cited by 11 publications

(16 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A total of 60 publications were identified in the databases; however, 31 were duplicate articles. Among the 29 articles selected, 20 were excluded based on the titles and abstracts because they did not meet the inclusion criteria: 2 were literature reviews [ 7 , 24 ]; 6 did not study açaí in an animal model of cancer and/or did not perform a toxicological analysis [ 25 – 30 ]; 10 were in vitro studies [ 13 , 20 , 31 – 38 ]; and 2 did not use the order Rodentia [ 21 , 39 ]. After reading the full texts 9 articles were included for their critical evaluations of the safety and effectiveness of açaí in animal experimental models [ 40 – 48 ].…”

Section: Resultsmentioning

confidence: 99%

“…To date, açaí has been shown to have pharmacological properties including antiinflammatory, antioxidant, cardioprotective and anticancer activities [ 1 , 7 – 9 , 18 , 19 ]. Furthermore, açaí was not shown to be genotoxic in vitro and in vivo studies conducted, in cultured human lymphocytes and hepatoma cell lines [ 20 ], in rodents [ 21 ] and in humans [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anticancer potential, molecular mechanisms and toxicity of Euterpe oleracea extract (açaí): A systematic review

et al. 2018

View full text Add to dashboard Cite

Cancer is an increasingly frequent malignancy worldwide, and despite the advances in drug development, it is still necessary to develop new plant-derived medicines. Euterpe oleracea (açaí) is abundant in South and Central America and has health benefits due to its high levels of phytochemicals, including lignans and polyphenols. The aim of this review was to systematically describe the safety and antitumor effects of açaí in preclinical models using rodents to provide a more comprehensive assessment of açaí for both therapeutic uses and the development of future clinical studies in cancer. Eligible studies were identified using four international databases (PubMed, Medline, Lilacs and SciELO) from their inception date through December 2017. The included studies were analyzed with methodological rigor (QATRS) to enable better quality control for these experimental studies. Sixty publications were identified in the databases, but only 9 articles were eligible: 6 evaluated the pharmacological effects of açaí in animal models of cancer (1 model each of esophageal cancer, urothelial cancer, melanoma and Walker-256 tumor and 2 models of colon cancer), and 3 were toxicological assays using preclinical models with rodents. Overall, 747 animals were analyzed. On a QATRS score scale of 0–20, the quality of the studies ranged from 16 to 20 points. Pulp was the main fraction of açaí administered, and an oral administration route was most common. The açaí dosage administered by gavage ranged from 30 mg/kg to 40,000 mg/kg, and açaí fed in the diet accounted for 2.5% to 5% of the diet. The anticarcinogenic and chemopreventive activities of açaí were observed in all experimental models of cancer and reduced the incidence, tumor cell proliferation, multiplicity and size of the tumors due to the antiinflammatory, antiproliferative and proapoptotic properties of açaí. No genotoxic effects were observed after açaí administration. The results of this review suggest that açaí is safe and can be used as a chemoprotective agent against cancer development. Açaí therapy may be a novel strategy for treating cancer.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anticancer potential, molecular mechanisms and toxicity of Euterpe oleracea extract (açaí): A systematic review

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Edible mushrooms are regarded as non-toxic and safe, but recent investigations reported that some of them cause DNA damages [27]. Numerous studies showed that the CBMN assay is a valid test for detection of genomic damage caused both in vitro [28,29] and in vivo [30,31]. Our results of CBMN assay showed that the extract was genotoxic in higher tested concentrations.…”

Section: Cytotoxic Activitymentioning

confidence: 55%

Investigation of Biological Activities and Secondary Metabolites of Hydnum Repandum Acetone Extract

Tubic¹

2019

FARMACIA

View full text Add to dashboard Cite

This study aimed to evaluate the biological activities and polyphenolic contents of the acetone extract of H. repandum mushroom. Polyphenolic compounds were evaluated by high-performance liquid chromatography (HPLC). The antioxidant activity was assessed by radical scavenging activity assays and reducing power. The antimicrobial activity was established based on the values of the minimum inhibitory concentration (MIC) using microdilution method. Cytotoxic activity was determined by 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT). The genotoxic and antimutagenic activities were tested using cytokinesis block micronucleus (CBMN) assay on human peripheral blood lymphocytes in vitro. Among the determined polyphenolic compounds, ferulic acid and quercetin were mostly found. The extract showed high free radical scavenging activity, while the reducing power was less emphasized and concentration-dependent. The MIC fluctuated in a range of 0.009-10 mg/mL. The cytotoxic activity (based on IC 50 ) ranged from 116.5 to 158.33 µg/mL, when HeLa cells were the most sensitive. The highest tested concentrations of the extract showed significant genotoxic activity, while against mitomycin C, the extract caused protective activity. The results indicated that H. repandum acetone extract contained secondary metabolites which showed biological activities such as antioxidant, antimicrobial, cytotoxic, genotoxic and protective against chemotherapeutics, indicating that their inclusion in nutrition could be of great importance in the prevention and treatment of various pathological conditions. RezumatAcest studiu a urmărit evaluarea activităților biologice și conținutul polifenolic al extractului acetonic din ciuperci din specia H. repandum. Compușii polifenolici au fost evaluați prin cromatografie de lichide de înaltă performanță (HPLC). Activitatea antioxidantă a fost evaluată prin testele specifice. Activitatea antimicrobiană a fost stabilită pe baza valorilor concentrației inhibitorii minime (MIC) utilizând metoda microdiluției. Activitatea citotoxică a fost determinată cu ajutorul bromurii de 3-4,5-dimetiltiazol-2-il-2,5-difeniltetrazoliu (MTT). Activitățile genotoxice și antimutagene au fost testate in vitro pe limfocite din sânge periferic. Printre compușii polifenolici determinați s-au regăsit predominant acid ferulic și quercetină. Extractul a demonstrat o activitate mai puternică de neutralizarea a radicalilor liberi. MIC a fluctuat în intervalul 0,009-10 mg/mL. Activitatea citotoxică (pe baza IC50) a variat de la 116,5 până la 158,33 µg/mL. Cele mai mari concentrații testate ale extractului au prezentat o activitate genotoxică semnificativă, în timp ce extractul de mitomicină C a prezentat o activitate protectoare. Rezultatele au arătat că extractul acetonic al H. repandum conține metaboliți secundari care au prezentat activități biologice antioxidante, antimicrobiene, citotoxice, genotoxice și protectoare împotriva chimioterapicelor, indicând faptul că includerea lor în nutriție ar putea avea o mare importanț...

show abstract

“…However, it has been reported that phenolic compounds, especially those belonging to the anthocyanin class, are able to help in the prevention and/or treatment of the disease. As a genotoxic assessment revealed the absence of significant DNA and chromosome damage in human HepG2 cells when treated with açai extract [ 16 ], we used HepG2 cells to mimic steatosis [ 17 ], and primary adipocyte cells, treated with TNF-α, as a model of persistent subcutaneous fat accumulation induced by an inflammatory state in vitro [ 18 ]. In the present study, we analyzed the effect of açai extract on the reduction of inflammatory states, highlighted its ability to modulate oxidative stress in combination with fat deposit accumulation, and revealed its potent effect to reduce key biomarkers related to fat accumulation.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Hypolipidemic Activity of Açai Fruit Makes It a Valuable Functional Food

et al. 2020

View full text Add to dashboard Cite

Several plant extracts are acquiring increasing value because of their antioxidant activity and hypolipidemic properties. Among them, great interest has been recently paid to açai fruit as a functional food. The aim of this study was to test the ability of açai extract in reducing oxidative stress and modulating lipid metabolism in vitro using different cell models and different types of stress. In fact, lipid peroxidation as evaluated in a HepG2 model was reduced five-fold when using 0.25 µg/mL of extract, and it was further reduced (20-fold) with the concentration increase up to 2.5 µg/mL. With the nonalcoholic fatty liver disease (NAFLD)in vitro model, all concentrations tested showed at least a two-fold reduced fat deposit. In addition, primary adipocytes challenged with TNF-α under hypoxic conditions to mimic the persistent subcutaneous fat, treated with açai extract showed an approximately 40% reduction of fat deposit. Overall, our results show that açai is able to counteract oxidative states in all the cell models analysed and to prevent the accumulation of lipid droplets. No toxic effects and high stability overtime were highlighted at the concentrations tested. Therefore, açai can be considered a suitable support in the prevention of different alterations of lipid and oxidative metabolism responsible for fat deposition and metabolic pathological conditions.

show abstract

Research Article First cytotoxic, genotoxic, and antigenotoxic assessment of Euterpe oleracea fruit oil (açaí) in cultured human cells.

Cited by 11 publications

References 20 publications

Anticancer potential, molecular mechanisms and toxicity of Euterpe oleracea extract (açaí): A systematic review

Anticancer potential, molecular mechanisms and toxicity of Euterpe oleracea extract (açaí): A systematic review

Investigation of Biological Activities and Secondary Metabolites of Hydnum Repandum Acetone Extract

Antioxidant and Hypolipidemic Activity of Açai Fruit Makes It a Valuable Functional Food

Contact Info

Product

Resources

About