Research Article Correlation analysis of ADAMTS-4, VCAM-1, and TAK1 expression in cartilage tissue from spine tuberculosis.

Qiu, Zhao; Sha, Zheng; Che, Xiaoming; Wang, M.Y.

doi:10.4238/gmr16038961

Cited by 6 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MMPs-1, -3 and 10 were similarly the most significantly acidosis upregulated genes amongst Collagen Degradation and ECM degradation genes. After MMP genes, the next most upregulated gene in these sets was protease ADAMTS4 which also degrades ECM components and is highly expressed at sites of TB infection [ 39 ]. Transcriptome profiling therefore demonstrates that acidosis enhances expression of ECM degradation pathways in M.tb infected MDMs by increasing expression of key MMPs of known importance to TB immunopathology.…”

Section: Resultsmentioning

confidence: 99%

An acidic microenvironment in Tuberculosis increases extracellular matrix degradation by regulating macrophage inflammatory responses

Whittington,

Turner,

Baark

et al. 2023

PLoS Pathog

View full text Add to dashboard Cite

Mycobacterium tuberculosis (M.tb) infection causes marked tissue inflammation leading to lung destruction and morbidity. The inflammatory extracellular microenvironment is acidic, however the effect of this acidosis on the immune response to M.tb is unknown. Using RNA-seq we show that acidosis produces system level transcriptional change in M.tb infected human macrophages regulating almost 4000 genes. Acidosis specifically upregulated extracellular matrix (ECM) degradation pathways with increased expression of Matrix metalloproteinases (MMPs) which mediate lung destruction in Tuberculosis. Macrophage MMP-1 and -3 secretion was increased by acidosis in a cellular model. Acidosis markedly suppresses several cytokines central to control of M.tb infection including TNF-α and IFN-γ. Murine studies demonstrated expression of known acidosis signaling G-protein coupled receptors OGR-1 and TDAG-8 in Tuberculosis which are shown to mediate the immune effects of decreased pH. Receptors were then demonstrated to be expressed in patients with TB lymphadenitis. Collectively, our findings show that an acidic microenvironment modulates immune function to reduce protective inflammatory responses and increase extracellular matrix degradation in Tuberculosis. Acidosis receptors are therefore potential targets for host directed therapy in patients.

show abstract

Section: Resultsmentioning

confidence: 99%

An acidic microenvironment in Tuberculosis increases extracellular matrix degradation by regulating macrophage inflammatory responses

Whittington,

Turner,

Baark

et al. 2023

PLoS Pathog

View full text Add to dashboard Cite

show abstract

“…The activity of superoxide dismutase (SOD) was determined as described by Zhang et al (2012b) and the content of malondialdehyde (MDA) was assayed using the protocol from a previous study (Feng et al, 2009b). The correlation analyses of the differences between sampling days and concentrations of SOD and MDA were performed by SPSS 19 (Qiu et al, 2017).…”

Section: Measurement Of Physiological and Biochemical Indexesmentioning

confidence: 99%

Transcriptome sequencing and analysis reveals the molecular response to selenium stimuli in Pueraria lobata (willd.) Ohwi

Guo

Yao

Yang

et al. 2020

PeerJ

View full text Add to dashboard Cite

Pueraria lobata (willd.) Ohwi is a consumable selenium-enriched plant used for medicinal purposes. The molecular response to selenium (Se) stimuli in P. lobata is currently unknown. We used RNA-Seq to identify potential genes involved in selenite metabolism and analyzed their expression profiles. We obtained a total of 150,567 unigenes, of which 90,961 were annotated, including 16 structural genes, 14 sulfate transporters, and 13 phosphate transporters that may be involved in Se metabolism, and 33 candidate structural genes involved in isoflavone biosynthesis. The genes with a —foldchange— >2 and q value <0.05 after sodium selenite treatment were identified as differentially expressed genes (DEGs). We obtained a total of 4,246 DEGs, which were enriched in GO terms that included “response to stimulus”, “response to stress”, “signal transduction”, “response to abiotic stimulus”, and “response to chemical”. Of the 4,246 DEGs, one sulfate transporter and five phosphate transporter genes involved Se metabolism, and nine structural genes involved in isoflavone biosynthesis were up-regulated. The expression patterns of 10 DEGs were selected randomly and validated using qRT-PCR. The Pearson Correlation Coefficient (r) was 0.86, indicating the reliability of RNA-Seq results. 22 Reactive Oxygen Species (ROS) scavenging DEGs were found, 11 of which were up-regulated. 436, 624 transcription factors (TFs) correlated with structural genes were identified that may be involved in Se and isoflavone biosynthesis, respectively, using r (r > 0.7 or r < − 0.7). 556 TFs were related to at least one sulfate and phosphate transporter. Our results provided a comprehensive description of gene expression and regulation in response to Se stimuli in P. lobata.

show abstract

Section: Measurement Of Physiological and Biochemical Indexesmentioning

confidence: 99%

Peer Review #1 of "Transcriptome sequencing and analysis reveals the molecular response to selenium stimuli in Pueraria lobata (willd.) Ohwi (v0.2)"

2020

View full text Add to dashboard Cite

Pueraria lobata (willd.)Ohwi is a consumable selenium-enriched plant used for medicinal purposes. The molecular response to selenium (Se) stimuli in P. lobata is currently unknown. We used RNA-Seq to identify potential genes involved in selenite metabolism and analyzed their expression profiles. We obtained a total of 150,567 unigenes, of which 90,961 were annotated, including 16 structural genes, 14 sulfate transporters, and 13 phosphate transporters thatmay be involved in Se metabolism, and 33 candidate structural genes involved in isoflavone biosynthesis. The genes with a |foldchange| > 2 and q value < 0.05 after sodium selenite treatment were identified as differentially expressed genes(DEGs). We obtained a total of 4,246 DEGs, which were enriched in GO terms that included "responseto stimulus", " response to stress", "signal transduction", "response to abiotic stimulus", and "response tochemical". Of the 4,246 DEGs, 1 sulfate transporter and 5 phosphate transporter genes involved Semetabolism, and 9 structural genes involved in isoflavone biosynthesis were up-regulated. The expression patterns of 10 DEGs were selected randomly and validated using qRT-PCR. The Pearson Correlation Coefficient (r) was 0.86, indicating the reliability of RNA-Seq results. 22 Reactive Oxygen Species (ROS) scavenging DEGs were found, 11 of which were up-regulated. 436,624 transcription factors (TFs) correlate d with structural genes were identified that may be involved in Se and isoflavone biosynthesis, respectively, using r (r > 0.7 or r <-0.7). 556 TFs were related to at least one sulfate and phosphate transporter. Our results provided a comprehensive description of gene expression and regulation in response to Se stimuli in P. lobata .,

show abstract

Research Article Correlation analysis of ADAMTS-4, VCAM-1, and TAK1 expression in cartilage tissue from spine tuberculosis.

Cited by 6 publications

References 28 publications

An acidic microenvironment in Tuberculosis increases extracellular matrix degradation by regulating macrophage inflammatory responses

An acidic microenvironment in Tuberculosis increases extracellular matrix degradation by regulating macrophage inflammatory responses

Transcriptome sequencing and analysis reveals the molecular response to selenium stimuli in Pueraria lobata (willd.) Ohwi

Peer Review #1 of "Transcriptome sequencing and analysis reveals the molecular response to selenium stimuli in Pueraria lobata (willd.) Ohwi (v0.2)"

Contact Info

Product

Resources

About