Research Article Characterization and phylogenetic affiliation of Actinobacteria from tropical soils with potential uses for agro-industrial processes.

Dornelas, J.C.M.; Figueiredo, J. E. F.; Abreu, C. S. de; Lana, U. G. de P.; Oliveira, C. A. V.; Marriel, I. E.

doi:10.4238/gmr16039703

Cited by 5 publications

(8 citation statements)

References 32 publications

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“…Previous studies also revealed that actinomycetes are one of the known cellulose producers (Jang & Chen 2003). Considering that the amount of cellulose in the composting process is higher than in soil samples, Streptomyces is important in composting processes in terms of breaking down cellulose (Dornelas et al 2017). Furthermore, most isolates in this study showed strong protease, pectinase and lipase (Tween 20 as substrate) activities.…”

Section: Discussionmentioning

confidence: 99%

SCREENING OF ACTINOMYCETES FROM Cystoseira barbata (Stackhouse) C. Agardh COMPOST FOR THEIR ENZYME AND ANTIBACTERIAL ACTIVITIES

TOPATAN

Katı

2022

Trakya University Journal of Natural Sciences

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Secondary metabolites obtained from bacteria have an important role their diverse applications and use in various industrial fields. Today, the constant search for novel enzyme and antibiotic are carried out from actinomycetes obtained from different sources. Actinomycetes obtained from the extreme environments are known to be producers of novel enzymes and antibiotic with great industrial potential. In this study, Actinomycetes isolation was performed by serial dilution method from compost obtained Cystoseira sp. algae collected from Black Sea coast. Enzyme contents by qualitative method and antibacterial activities by using cross streak method of isolated actinomycetes were investigated. Identification of 6 actinomycetes isolates with high enzyme and antibacterial activity was performed according to morphological, physiological and 16S rDNA sequence analysis. A total of 73 actinomycetes isolates (BSC) were obtained from the compost samples. When the enzyme activities of actinomycetes isolates were evaluated, it is found that 68.5% of these have amylase, 100% have cellulase, 47.9% have chitinase, 94.5% have pectinase, 98.6% have protease and 96.3% have lipase/esterase enzyme production. The highest antibacterial activity was observed against Staphylococcus aureus and Enterococcus faecalis, which inhibited 98.6 and 84.9 % of the tested actinomycetes isolates, respectively. It has been determined that the actinomycetes isolates (BSC-13, BSC-17, BSC-37, BSC-38, BSC-45 and BSC-49) with high enzyme and antibacterial activity belong to Streptomyces genus according to 16S rDNA sequence analysis. Secondary metabolites from actinomycetes are likely to be used in different industrial areas in the future.

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Section: Discussionmentioning

confidence: 99%

SCREENING OF ACTINOMYCETES FROM Cystoseira barbata (Stackhouse) C. Agardh COMPOST FOR THEIR ENZYME AND ANTIBACTERIAL ACTIVITIES

TOPATAN

Katı

2022

Trakya University Journal of Natural Sciences

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“…Essa classificação foi feita por meio de comparação de imagens do Atlas de Actinomicetos, publicado pela Sociedade Japonesa de Actinomicetos e com o trabalho de Arai, 1976. A morfologia das cadeias de esporos foi classificada segundo Dornelas et al (2017).…”

Section: Análise Micromorfológicaunclassified

“…Na Figura 5, as estruturas predominantes foram flexíveis com 29% (07 cepas), espiral com 33% (08 cepas), linha reta 17% (04 cepas) e retículo 21% (05 cepas). Variação similar foi constatada por Dornelas et al (2017), ao documentarem a caracterização morfológica e afiliação filogenética de cepas de actinobactérias de solos tropicas de uma área de cerrado, no Estado de Minas Gerais.…”

Section: Cepasunclassified

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Morfologia De Cepas De Actinobactérias Em Áreas Suscetíveis À Desertificação

Santos¹,

Oliveira²,

Meneses³

et al. 2019

Enci Bio

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“…They include terrestrial habitats (topsoil, forest soil, plant rhizosphere soil, deserts, acid mine site, etc.) Fang et al 2015;Dornelas et al 2017), aquatic environments (deep sea sediments, superficial and deep seawater, river sediment, pond water, lake water, etc.) (Minegishi et al 2013;Zhang et al 2017), and eukaryotic-associated microbiomes (marine sponge, termite, rumen, human microbiota, etc.)…”

Section: Metagenomicsmentioning

confidence: 99%

Metagenomic approaches to discover lipolytic enzymes

Lu¹

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Stable in a Microorganism Pseudomonas aeruginosa san-ai 30 °C, 48 h 25 % (v/v) chloroform and n-hexane Karadzic et al. 2006 Bacillus sphaericus 205y 37 °C, 30 min 25 % (v/v) n-hexane and p-xylene. Hun et al. 2003 Bacillus megaterium 29 °C, 1 h 25-80 % (v/v) ethanol and acetone 100 % (v/v) 2-propanol, 1-butanol, Tol, n-hexane and n-heptane Lima et al. 2004 Sulfolobus solfataricus P1 30 °C or 70 °C, 1 h 40 % (v/v) methanol, ethanol and 2propanol. Mandrich et al. 2005 Stenotrophomonas maltophilia CGMCC 4254 30 °C, 24 h 20 and 50 % (v/v) benzene, toluene, nhexane and n-heptane. Li et al. 2013 Pseudomonas aeruginosa MH38 25 °C, 1 h 30 and 50 % (v/v) benzene and hexane Jang et al. 2014 Monascus purpureus 40 °C, 24 h 20 % (v/v) methanol, ethanol, acetonitrile, glycerol, acetone, n-Hexane, toluene and chloroform Kang et al. 2017 Chromohalobacter sp. 35 °C, 30 min 20 and 50 % (v/v) benzene and hexane Ai et al. 2018 Psychrobacter sp. ZY124 37 °C, 5 h 10, 30 and 50 % (v/v) DMSO, methanol, ethanol, acetone, acetonitrile, toluene, pentane, hexane and octane Zhang et al. 2018b Metagenome Marine mud 30 °C, 12 h 20 % (v/v) ethanol, acetonitrile, DMF and cyclohexane Gao et al. 2016 Forest soil sample. 37 °C, 2 h 25 % (v/v) DMSO, Benzene and p-xylene Berlemont et al. 2013 Wastewater treatment plant of a meat packing and dairy industry 4 °C, 48 h 15 and 30 % (v/v) methanol, ethanol, 1propanol, 2-propanol, glycerol, THF, dioxane, DMSO Glogauer et al. 2011 Soil 30 °C, 2 h 15 and 30 % (v/v) DMSO, DMF, p-xylene, hexane, heptane, and octane Wang et al. 2013 Compost Room temp., 26 d 30 % (v/v) methanol, ethanol, isopropanol, DMSO, acetone Lu et al., 2019 Soil contaminated with petroleum hydrocarbons No data 30 % (v/v) DMF and DMSO Pereira et al. 2015 a Abbreviations for the organic solvents are: DMF, N,N-dimethylformamide; DMSO, dimethylsulfoxide; THF, tetrahydrofuran. Halotolerance Among extremophilic LEs, halophilic/halotolerant LEs are another major group of industrial relevant enzymes. In comparison to the non-halophilic/non-halotolerant Source of LEs Enzyme properties Reference Salt range Maximum activity (%) a Microorganism Pelagibacterium halotolerans 0-4 M ~ 160 % at 3 M Jiang et al. 2012 Alcanivorax borkumensis 0-3.5 M 100 % at 0 M Tchigvintsev et al. 2015 Serratia sp. 0-4 M 100 % at 0 M Jiang et al. 2016 Alkalibacterium sp. 0-4 M ~ 105 % at 2 M Wang et al. 2016 Psychrobacter pacificensis 0-5 M 143.2 % at 2 M Wu et al. 2013a Lactobacillus plantarum 0-25 % ~ 250 % at 1M Esteban-Torres et al. 2014 Zunongwangia profunda 0-4.5 M 100 % at 0 M Rahman et al. 2016 Haloarcula marismortui 0-5 M 800 mU at 3 M Rao et al. 2009 Thalassospira sp. 0-4 M 283 % at 3 M De Santi et al. 2016b Bacillus licheniformis 0-5 M 588 % at 3.5 M Zhang et al. 2018 Metagenome Deep sea sponge 0-24% 100 % at 0 M Borchert et al. 2017 Marine sponge 0-4 M 234 % at 5 M Selvin et al. 2012 Soil 0-5 M 155 % at 1 M Jayanath et al. 2018 Marine arctic sediment 0-4 M 675 % at 3 M De Santi et al. 2016a Deep-sea shrimp 0-4 M ~ 250 % at 3.2 M Alcaide et al. 2015b 0-4 M ~ 250 %...

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Research Article Characterization and phylogenetic affiliation of Actinobacteria from tropical soils with potential uses for agro-industrial processes.

Cited by 5 publications

References 32 publications

SCREENING OF ACTINOMYCETES FROM Cystoseira barbata (Stackhouse) C. Agardh COMPOST FOR THEIR ENZYME AND ANTIBACTERIAL ACTIVITIES

SCREENING OF ACTINOMYCETES FROM Cystoseira barbata (Stackhouse) C. Agardh COMPOST FOR THEIR ENZYME AND ANTIBACTERIAL ACTIVITIES

Morfologia De Cepas De Actinobactérias Em Áreas Suscetíveis À Desertificação

Metagenomic approaches to discover lipolytic enzymes

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