Research Article Analysis of p53 gene polymorphism (codon 72) in symptomatic patients with atherosclerosis.

Abstract

ABSTRACT. Atherosclerosis is a multifactorial pathological disease that alters the morphology and function of arterial walls. The atheroma growth leads to vessel hardening and lumen narrowing, limiting the blood flow. The atheroma plaque can eventually break, expose highly thrombogenic material and lead to platelet activation and subsequent formation of a thrombus that may block blood flow in loco, or even leading to obstruction of other vessels with a smaller diameter. This process is one of the main determinants of the clinical manifestations of atherosclerosis, such as coronary artery disease, ischemic stroke, and peripheral arterial disease. Although the inflammatory theory about atherosclerosis is the most renowned one, observations point to common biological characteristics between cancer and atherosclerosis suggesting a possible association between p53 and atherosclerotic diseases. We collected peripheral blood samples from 200 individuals with clinical manifestations of atherosclerotic disease and 100 individuals without manisfestation of the disease to form the control group. DNA was subjected to molecular analysis (PCR) to identify the polymorphism of the p53 gene. We have not found any relationship between the polymorphism of the p53 gene and atherosclerosis in the population studied (P = 0.36). There was no relationship between atherosclerosis, polymorphism of p53 and the variables accounted: smoking habit (P = 0.72, 0.51 and 0.62 for smokers, non-smokers and former smokers respectively), alcohol consumption (P = 0.17 for individuals with drinking habits and 0.38 for those who do not consume alcohol beverage), systemic arterial hypertension (P = 0.60), diabetes mellitus (P = 0.34), and dyslipidemia (P = 0.89). Our population has a high rate of miscegenation and heterozygotes, and according to studies the arginine variant is more related to plaque formation because it induces apoptosis more frequently when compared to the proline variant. According to our results, there is no association between the polymorphism of the p53 gene, atherosclerosis and its risk factors in the population studied.

TP53 single nucleotide polymorphism (rs1042522) in Iranian patients with coronary artery disease

“…In the Kuwaiti population, CAD prevalence exhibited an association with type 2 diabetes and high levels of triglyceride and cholesterol; however, there was no evidence of any influence of TP53 codon 72 genotypes (31). In Brazilian symptomatic CAD patients, neither atherosclerosis nor the risk factors were associated with Arg72Pro genotypes (63). Contrary with the current findings, Khan et al (35) reported Arg72 allele as a risk factor for CAD in South African (Indian ancestry) patients, but in line with the present study, the laboratory markers did not correlate with the genotypes.…”

Therapeutic Design of Peptide Modulators of the Interaction Between eNOS and p53 in Atherosclerosis

“…Regarding the p53 protein, which is coded by the TP53 gene, is known as the guardian of the genome. The protein is a classical tumor suppressor protein related to cancer and several other diseases related to genomic instability, such as endometriosis, atherosclerosis and infertility [58][59][60][61]. Experimental approaches along with bioinformatic tools have contributed to increase our knowledge regarding diseases, development of new diagnosis and therapeutic strategies [62,63].…”

Dna Barcoding Confirma a Ocorrência De Espécies Amazônicas Na Ictiofauna Do Rio Turiaçu, Maranhão/Brasil

“…Ela é uma proteína importante que atua como supressora de tumores, e assim, intimamente ligada ao câncer (MOBARAKI et al, 2018). Variações gênicas nas regiões codificadoras do gene TP53 também estão relacionadas a doenças ligadas a perda do controle do ciclo celular, como a endometriose (SILVA; MOURA, 2016a), aterosclerose (LAGARES et al, 2017), e infertilidade (DE MORAIS et al, 2016). Outras funções da p53 dizem respeito à regulação e progressão do ciclo celular, além de proteger o DNA contra danos internos e externos (SPEIDEL, 2015).…”