Research and project activities for breeding of <i>Cryptomeria japonica</i> D. Don in Japan

Takahashi, Makoto; Miura, Masahiro; Fukatsu, Eitaro; Hiraoka, Yuichiro; Kurita, Masayuki

doi:10.1080/13416979.2023.2172794

Cited by 5 publications

(3 citation statements)

References 109 publications

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“…Therefore, planting pollen-free trees is a promising strategy for maintaining Japanese cedar as a building material while countering its effects in pollinosis. According to these situations and demand, several pollen-free cultivars were bred ( 20 ), and the annual production of seedlings of low-pollen and pollen-free Japanese cedar has been increasing yearly in Japan ( 21 ). Unveiling the genetic mechanism of pollen-free mutants will contribute not only to promoting pollen-free timber breeding but also to understanding the flowering mechanism in gymnosperm plants.…”

Section: Introductionmentioning

confidence: 99%

A single-nucleotide substitution of CjTKPR1 determines pollen production in the gymnosperm plant Cryptomeria japonica

et al. 2023

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Pollinosis, also known as pollen allergy or hay fever, is a global problem caused by pollen produced by various plant species. The wind-pollinated Japanese cedar (Cryptomeria japonica) is the largest contributor to severe pollinosis in Japan, where increasing proportions of people have been affected in recent decades. The MALE STERILITY 4 (MS4) locus of Japanese cedar controls pollen production, and its homozygous mutants (ms4/ms4) show abnormal pollen development after the tetrad stage and produce no mature pollen. In this study, we narrowed down the MS4 locus by fine mapping in Japanese cedar and found TETRAKETIDE α-PYRONE REDUCTASE 1 (TKPR1) gene in this region. Transformation experiments using Arabidopsis thaliana showed that single-nucleotide substitution (“T” to “C” at 244-nt position) of CjTKPR1 determines pollen production. Broad conservation of TKPR1 beyond plant division could lead to the creation of pollen-free plants not only for Japanese cedar but also for broader plant species.

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Section: Introductionmentioning

confidence: 99%

A single-nucleotide substitution of CjTKPR1 determines pollen production in the gymnosperm plant Cryptomeria japonica

et al. 2023

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“…This work investigates the potential utility of GP in C. japonica, the most important forestry tree species in Japan: it is planted widely in all parts of the country, other than Okinawa, accounting for about 44% of Japan's planted forest area [19]. Eastern Japan is dominated by seedling forestry derived from seed gardens, while western Japan, where this study was conducted, is dominated by clonal forestry based on cuttings from scion gardens [20]. Clonal forestry has been practiced in this region for over 500 years [21], and clonal values, i.e., genotypic values, are commonly estimated in clonal test sites to select plus trees.…”

Section: Introductionmentioning

confidence: 99%

“…Pollen-related characteristics such as male sterility and low pollen fecundity are important breeding targets for C. japonica in Japan because allergic reactions to its pollen have recently become a severe public health problem, affecting at least 26.5% of the Japanese population according to a nationwide epidemiological survey [22]. To address this problem, forest research institutes and forestry agencies in various regions have developed and made available male sterile individuals and individuals with low pollen fertility [20,23,24]. Male flower quantity is generally assessed after 15 years or more when trees have reached maturity.…”

Section: Introductionmentioning

confidence: 99%

Genomic Prediction of Tree Height, Wood Stiffness, and Male Flower Quantity Traits across Two Generations in Selected Individuals of Cryptomeria japonica D. Don (Japanese Cedar)

Ejima,

Uchiyama,

Mori

et al. 2023

Forests

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Breeding long-lived trees is challenging, but it has been shown that genomic information can be used to improve efficiency. In this study, genomic prediction (GP) was tested on selected individuals of a two-generation breeding population of Cryptomeria japonica, the most common plantation tree in Japan. In the 1980s, the second-generation plus trees (101 clones) were selected from about 8500 individuals obtained by cross-mating the first-generation plus trees (47 clones). RAD-seq based on 8664 SNPs was used to perform GP for three important traits in this population: tree height, wood stiffness, and male flower quantity. The association between traits and genotypes was modeled using five Bayesian models whose predictive accuracy was evaluated by cross-validation, revealing that the best model differed for each trait (BRR for tree height, BayesA for wood stiffness, and BayesB for male flower quantity). GP was 1.2–16.0 times more accurate than traditional pedigree-based methods, attributed to its ability to model Mendelian sampling. However, an analysis of the effects of intergenerational kinship showed that parent–offspring relationships reduce the predictive accuracy of GP for traits strongly affected by selection pressure. Overall, these results show that GP could significantly expedite tree breeding when supported by a deep understanding of the targeted population’s genetic background.

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Development and application of a KASP marker for marker-assisted selection against the male-sterile gene MALE STERILITY 4 (MS4) in Japanese cedar (Cryptomeria japonica D. Don)

Watanabe,

Ueno,

Hasegawa

et al. 2024

New Forests

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Research and project activities for breeding of Cryptomeria japonica D. Don in Japan

Cited by 5 publications

References 109 publications

A single-nucleotide substitution of CjTKPR1 determines pollen production in the gymnosperm plant Cryptomeria japonica

A single-nucleotide substitution of CjTKPR1 determines pollen production in the gymnosperm plant Cryptomeria japonica

Genomic Prediction of Tree Height, Wood Stiffness, and Male Flower Quantity Traits across Two Generations in Selected Individuals of Cryptomeria japonica D. Don (Japanese Cedar)

Development and application of a KASP marker for marker-assisted selection against the male-sterile gene MALE STERILITY 4 (MS4) in Japanese cedar (Cryptomeria japonica D. Don)

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