Rescuing recombinant proteins by sequestration into the P22 VLP

Patterson, Dustin P.; LaFrance, Benjamin; Douglas, Trevor

doi:10.1039/c3cc46517a

Cited by 44 publications

(61 citation statements)

References 21 publications

(39 reference statements)

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“…431 The strategy for sequestration of enzymes within P22 during expression is also valuable for the recovery of otherwise insoluble proteins. 432 Some recombinant proteins are trafficked to inclusion bodies during production in E. coli , making recovery difficult. α-galactosidase (GalA) is one such protein that was studied, and encapsulation within P22 was shown to allow for successful rescue of properly folded GalA.…”

Section: Applications Of Virus-based Particlesmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

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Virus-based nanomaterials are versatile materials that naturally self-assemble and have relevance for a broad range of applications including medicine, biotechnology, and energy. This review provides an overview of recent developments in “chemical virology.” Viruses, as materials, provide unique nanoscale scaffolds that have relevance in chemical biology and nanotechnology, with diverse areas of applications. Some fundamental advantages of viruses, compared to synthetically programmed materials, include the highly precise spatial arrangement of their subunits into a diverse array of shapes and sizes and many available avenues for easy and reproducible modification. Here, we will first survey the broad distribution of viruses and various methods for producing virus-based nanoparticles, as well as engineering principles used to impart new functionalities. We will then examine the broad range of applications and implications of virus-based materials, focusing on the medical, biotechnology, and energy sectors. We anticipate that this field will continue to evolve and grow, with exciting new possibilities stemming from advancements in the rational design of virus-based nanomaterials.

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Section: Applications Of Virus-based Particlesmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

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“…These viral platforms were intended to be used as imaging probes. Many proteins have also been entrapped within VLPs, such as bacteriophage Qβ [31] and bacteriophage P22 [32][33][34][35][36], in order to solubilize, stabilize and protect them. Protein encapsulation is generally accomplished either by in vitro packaging through disassembly and reassembly of VLPs in the presence of protein cargo or through genetic fusion of the scaffold protein to the cargo.…”

Section: Drug Encapsulation Through Noncovalent Interactionsmentioning

confidence: 99%

Synthetic and Bioinspired Cage Nanoparticles for Drug Delivery

Deshayes

Gref

2014

Nanomedicine

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Nanotechnology has the potential to revolutionize drug delivery, but still faces some limitations. One of the main issues regarding conventional nanoparticles is their poor drug-loading and their early burst release. Thus, to overcome these problems, researchers have taken advantage of the host-guest interactions that drive some assemblies to form cage molecules able to strongly entrap their cargo and design new nanocarriers called cage nanoparticles. These systems can be classified into two categories: bioinspired nanosystems such as virus-like particles, ferritin, small heat shock protein: and synthetic host-guest supramolecular systems that require engineering to actually form supramolecular nanoassemblies. This review will highlight the recent advances in cage nanoparticles for drug delivery with a particular focus on their biomedical applications.

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“…In some cases the VLP has been shown to enhance the solubility of recombinant proteins that are otherwise localized to inclusion bodies (Patterson et al, 2013a). VLPs have been shown to increase resistance to protease, thermal, and chemical denaturation (Fiedler et al, 2010).…”

Section: Vlps As Metabolic Compartmentsmentioning

confidence: 99%

“…The notable success of single-enzyme encapsulation toward stabilizing and protecting the enzyme cargo suggests that the most ubiquitous application of this technology is toward prolonging the lifespan of enzymes as useful catalysts. In addition the impressive ability to recover insoluble enzymes or notoriously unstable enzymes by sequestration in a VLP suggests that single-enzyme encapsulation for improved kinetics is best directed at the notoriously hard to handle enzymes and not at the well-behaved model enzymes that have dominated studies thus far (Jordan et al, 2016; Patterson et al, 2013). Additional potential for kinetic enhancement rests in the ability to encapsulate multiple, sequential enzymes in the same capsid.…”

Section: Vlps As Metabolic Compartmentsmentioning

confidence: 99%

Biomedical and Catalytic Opportunities of Virus-Like Particles in Nanotechnology

Schwarz

Uchida

Douglas

2017

Advances in Virus Research

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Within biology, molecules are arranged in hierarchical structures that coordinate and control the many processes that allow for complex organisms to exist. Proteins and other functional macromolecules are often studied outside their natural nanostructural context because it remains difficult to create controlled arrangements of proteins at this size scale. Viruses are elegantly simple nanosystems that exist at the interface of living organisms and nonliving biological machines. Studied and viewed primarily as pathogens to be combatted, viruses have emerged as models of structural efficiency at the nanoscale and have spurred the development of biomimetic nanoparticle systems. Virus-like particles (VLPs) are noninfectious protein cages derived from viruses or other cage-forming systems. VLPs provide incredibly regular scaffolds for building at the nanoscale. Composed of self-assembling protein subunits, VLPs provide both a model for studying materials’ assembly at the nanoscale and useful building blocks for materials design. The robustness and degree of understanding of many VLP structures allow for the ready use of these systems as versatile nanoparticle platforms for the conjugation of active molecules or as scaffolds for the structural organization of chemical processes. Lastly the prevalence of viruses in all domains of life has led to unique activities of VLPs in biological systems most notably the immune system. Here we discuss recent efforts to apply VLPs in a wide variety of applications with the aim of highlighting how the common structural elements of VLPs have led to their emergence as paradigms for the understanding and design of biological nanomaterials.

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Rescuing recombinant proteins by sequestration into the P22 VLP

Cited by 44 publications

References 21 publications

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Synthetic and Bioinspired Cage Nanoparticles for Drug Delivery

Biomedical and Catalytic Opportunities of Virus-Like Particles in Nanotechnology

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