Rescuing Nucleus Pulposus Cells From Senescence via Dual‐Functional Greigite Nanozyme to Alleviate Intervertebral Disc Degeneration

Shi, Yu; Li, Hanwen; Chu, David; Lin, Wenzheng; Wang, Xinglong; Yin, Wu; Li, Ké; Li, Dandan; Xu, Zhuobin; Gao, Lizeng; Li, Bin; Chen, Hao

doi:10.1002/advs.202300988

Cited by 12 publications

(1 citation statement)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hydrogel improved the metabolism of the NPCs matrix, which was conducive to the long-term treatment of IDD. In addition, more and more research has shown that abnormal intracellular reactive oxygen species (ROS) levels are associated with NPSCs aging and are also a significant cause of IDD [122][123][124]. IDD can be slowed down by controlling the source ROS levels.…”

Section: Intervertebral Disc Degenerationmentioning

confidence: 99%

Beyond Traditional Medicine: EVs-Loaded Hydrogels as a Game Changer in Disease Therapeutics

Du,

Zhou,

Zheng

2024

Gels

View full text Add to dashboard Cite

Extracellular vesicles (EVs), especially exosomes, have shown great therapeutic potential in the treatment of diseases, as they can target cells or tissues. However, the therapeutic effect of EVs is limited due to the susceptibility of EVs to immune system clearance during transport in vivo. Hydrogels have become an ideal delivery platform for EVs due to their good biocompatibility and porous structure. This article reviews the preparation and application of EVs-loaded hydrogels as a cell-free therapy strategy in the treatment of diseases. The article also discusses the challenges and future outlook of EVs-loaded hydrogels.

show abstract

Section: Intervertebral Disc Degenerationmentioning

confidence: 99%

Beyond Traditional Medicine: EVs-Loaded Hydrogels as a Game Changer in Disease Therapeutics

Du,

Zhou,

Zheng

2024

Gels

View full text Add to dashboard Cite

show abstract

Rescuing Nucleus Pulposus Cells from ROS Toxic Microenvironment via Mitochondria‐Targeted Carbon Dot‐Supported Prussian Blue to Alleviate Intervertebral Disc Degeneration

Shi,

Bu,

Chu

et al. 2024

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

Intervertebral disc degeneration (IVDD) is invariably accompanied by excessive accumulation of reactive oxygen species (ROS), resulting in progressive deterioration of mitochondrial function and senescence in nucleus pulposus cells (NPCs). Significantly, the main ROS production site in non‐immune cells is mitochondria, suggesting mitochondria is a feasible therapeutic target to reverse IVDD. Triphenylphosphine (TPP), which is known as mitochondrial‐tropic ligands, is utilized to modify carbon dot‐supported Prussian blue (CD‐PB) to scavenge superfluous intro‐cellular ROS and maintain NPCs at normal redox levels. CD‐PB‐TPP can effectively escape from lysosomal phagocytosis, permitting efficient mitochondrial targeting. After strikingly lessening the ROS in mitochondria via exerting antioxidant enzyme‐like activities, such as superoxide dismutase, catalase, etc CD‐PB‐TPP rescues damaged mitochondrial function and NPCs from senescence, catabolism, and inflammatory reaction in vitro. Imaging evaluation and tissue morphology assessment in vivo suggest that disc height index, mean grey values of nucleus pulposus tissue, and histological morphology were significantly improved in the IVDD model after CD‐PB‐TPP locally performed. In conclusion, this study demonstrates that ROS‐induced mitochondrial dysfunction and senescence of NPCs leads to IVDD and the CD‐PB‐TPP possesses enormous potential to rescue this pathological process through efficient removal of ROS via targeting mitochondria, supplying a neoteric strategy for IVDD treatment.This article is protected by copyright. All rights reserved

show abstract

Catalytic Nanodots‐Driven Pyroptosis Suppression in Nucleus Pulposus for Antioxidant Intervention of Intervertebral Disc Degeneration

Sun,

Yan,

Dai

et al. 2024

Advanced Materials

View full text Add to dashboard Cite

Low back pain resulting from intervertebral disc degeneration (IVDD) is a prevalent global concern; however, its underlying mechanism remains elusive. Single‐cell sequencing analyses revealed the critical involvement of pyroptosis in IVDD. Considering the involvement of reactive oxygen species (ROS) as the primary instigator of pyroptosis and lack of an efficient intervention approach, this study developed carbonized Mn‐containing nanodots (MCDs) as ROS‐scavenging catalytic biomaterials to suppress pyroptosis of nucleus pulposus (NP) cells to efficiently alleviate IVDD. Catalytic MCDs have superior efficacy in scavenging intracellular ROS and rescuing homeostasis in the NP microenvironment compared with N‐acetylcysteine, a classical antioxidant. The data validates that pyroptosis plays a vital role in mediating the protective effects of catalytic MCDs against oxidative stress. Systematic in vivo assessments substantiate the effectiveness of MCDs in rescuing a puncture‐induced IVDD rat model, further demonstrating their ability to suppress pyroptosis. This study highlights the potential of antioxidant catalytic nanomedicine as a pyroptosis inhibitor and mechanistically unveils an efficient strategy for the treatment of IVDD.This article is protected by copyright. All rights reserved

show abstract

Rescuing Nucleus Pulposus Cells From Senescence via Dual‐Functional Greigite Nanozyme to Alleviate Intervertebral Disc Degeneration

Cited by 12 publications

References 80 publications

Beyond Traditional Medicine: EVs-Loaded Hydrogels as a Game Changer in Disease Therapeutics

Beyond Traditional Medicine: EVs-Loaded Hydrogels as a Game Changer in Disease Therapeutics

Rescuing Nucleus Pulposus Cells from ROS Toxic Microenvironment via Mitochondria‐Targeted Carbon Dot‐Supported Prussian Blue to Alleviate Intervertebral Disc Degeneration

Catalytic Nanodots‐Driven Pyroptosis Suppression in Nucleus Pulposus for Antioxidant Intervention of Intervertebral Disc Degeneration

Contact Info

Product

Resources

About