Rescue of recombinant peste des petits ruminants virus: creation of a GFP-expressing virus and application in rapid virus neutralization test

Hu, Qianqian; Chen, Weiye; Huang, Kehe; Baron, Michael D.; Bu, Zhigao

doi:10.1186/1297-9716-43-48

Cited by 57 publications

(66 citation statements)

References 30 publications

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“…The recombinant version of the vaccine strain of PPRV (rNigeria/75/1) has been previously described [55]. The mutant viruses rNigeria/75/1_ΔV and rNigeria/75/1_ΔC were made using the same full-length genome construct in which the PPRV P gene was engineered to prevent the expression of either the C or the V protein.…”

Section: Methodsmentioning

confidence: 99%

Control of the induction of type I interferon by Peste des petits ruminants virus

2017

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Peste des petits ruminants virus (PPRV) is a morbillivirus that produces clinical disease in goats and sheep. We have studied the induction of interferon-β (IFN-β) following infection of cultured cells with wild-type and vaccine strains of PPRV, and the effects of such infection with PPRV on the induction of IFN-β through both MDA-5 and RIG-I mediated pathways. Using both reporter assays and direct measurement of IFN-β mRNA, we have found that PPRV infection induces IFN-β only weakly and transiently, and the virus can actively block the induction of IFN-β. We have also generated mutant PPRV that lack expression of either of the viral accessory proteins (V&C) to characterize the role of these proteins in IFN-β induction during virus infection. Both PPRV_ΔV and PPRV_ΔC were defective in growth in cell culture, although in different ways. While the PPRV V protein bound to MDA-5 and, to a lesser extent, RIG-I, and over-expression of the V protein inhibited both IFN-β induction pathways, PPRV lacking V protein expression can still block IFN-β induction. In contrast, PPRV C bound to neither MDA-5 nor RIG-I, but PPRV lacking C protein expression lost the ability to block both MDA-5 and RIG-I mediated activation of IFN-β. These results shed new light on the inhibition of the induction of IFN-β by PPRV.

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Section: Methodsmentioning

confidence: 99%

Control of the induction of type I interferon by Peste des petits ruminants virus

2017

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“…negatively marking dominant epitopes in H commonly detected during sero-surveillance [60]. Two systems for making recombinant PPRV have been published [61,62], offering a promising route for the production of such novel marker, DIVA or heterologous vaccines. However, it remains to be seen how effective this approach could be for PPRV, and how stable these mutations are during livevaccine production.…”

Section: Development and Application Of Pprv Vaccinesmentioning

confidence: 99%

Future research to underpin successful peste des petits ruminants virus (PPRV) eradication

Baron

Diop

Njeumi

et al. 2017

Journal of General Virology

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Peste des petits ruminants virus (PPRV) is a significant pathogen of small ruminants and is prevalent in much of Africa, the Near and Middle East and Asia. Despite the availability of an efficacious and cheap live-attenuated vaccine, the virus has continued to spread, with its range stretching from Morocco in the west to China and Mongolia in the east. Some of the world's poorest communities rely on small ruminant farming for subsistence and the continued endemicity of PPRV is a constant threat to their livelihoods. Moreover, PPRV's effects on the world's population are felt broadly across many economic, agricultural and social situations. This far-reaching impact has prompted the Food and Agriculture Organization of the United Nations (FAO) and the World Organisation for Animal Health (OIE) to develop a global strategy for the eradication of this virus and its disease. PPRV is a morbillivirus and, given the experience of these organizations in eradicating the related rinderpest virus, the eradication of PPRV should be feasible. However, there are many critical areas where basic and applied virological research concerning PPRV is lacking. The purpose of this review is to highlight areas where new research could be performed in order to guide and facilitate the eradication programme. These areas include studies on disease transmission and epidemiology, the existence of wildlife reservoirs and the development of next-generation vaccines and diagnostics. With the support of the international virology community, the successful eradication of PPRV can be achieved.

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“…The reverse genetics approach developed of late [23,176,177] will likely provide important insights into the host range and molecular pathways involved in the pathogenesis and replication of PPRV.…”

Section: Pathogenesismentioning

confidence: 99%

Peste Des Petits Ruminants Virus Infection of Small Ruminants: A Comprehensive Review

Kumar

Maherchandani

Kashyap

et al. 2014

Viruses

170

168

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Peste des petits ruminants (PPR) is caused by a Morbillivirus that belongs to the family Paramyxoviridae. PPR is an acute, highly contagious and fatal disease primarily affecting goats and sheep, whereas cattle undergo sub-clinical infection. With morbidity and mortality rates that can be as high as 90%, PPR is classified as an OIE (Office International des Epizooties)-listed disease. Considering the importance of sheep and goats in the livelihood of the poor and marginal farmers in Africa and South Asia, PPR is an important concern for food security and poverty alleviation. PPR virus (PPRV) and rinderpest virus (RPV) are closely related Morbilliviruses. Rinderpest has been globally eradicated by mass vaccination. Though a live attenuated vaccine is available against PPR for immunoprophylaxis, due to its instability in subtropical climate (thermo-sensitivity), unavailability of required doses and insufficient coverage (herd immunity), the disease control program has not been a great success. Further, emerging evidence of poor cross neutralization between vaccine strain and PPRV strains currently circulating in the field has raised concerns about the protective efficacy of the existing PPR vaccines. This review summarizes the recent advancement in PPRV replication, its pathogenesis, immune response to vaccine and disease control. Attempts have also been made to highlight the current trends in understanding the host susceptibility and resistance to PPR.

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Rescue of recombinant peste des petits ruminants virus: creation of a GFP-expressing virus and application in rapid virus neutralization test

Cited by 57 publications

References 30 publications

Control of the induction of type I interferon by Peste des petits ruminants virus

Control of the induction of type I interferon by Peste des petits ruminants virus

Future research to underpin successful peste des petits ruminants virus (PPRV) eradication

Peste Des Petits Ruminants Virus Infection of Small Ruminants: A Comprehensive Review

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