Rescue of male fertility following faecal microbiota transplantation from alginate oligosaccharide-dosed mice

Zhang, Cong; Xiong, Bohui; Chen, Liang; Ge, Wei; Yin, Shen; Feng, Yanni; Sun, Zhongyi; Sun, Qing‐Yuan; Zhao, Yong; Shen, Wei; Zhang, Hongfu

doi:10.1136/gutjnl-2020-323593

Cited by 43 publications

(56 citation statements)

References 9 publications

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“…For example, a recent study revealed that malignant osteoblastic cells, which are characterized by high mesenchymal marker and transcription factor expression, may originate from any type of cell along the osteogenic differentiation pathway from mesenchymal stem cells in osteosarcoma [ 91 ]. MUC6 + TFF2 + spasmolytic polypeptide-expressing metaplasia, which may originate from gastric chief cells, is a state of precancerous lesions in gastric cancer [ 89 ]. In addition, oxidative phosphorylation plays a key role in breast cancer metastasis based on a comparison of primary tumors and micrometastatic tissue [ 95 ].…”

Section: Applications Of Single-cell Sequencing In Cancer Biologymentioning

confidence: 99%

Applications of single-cell sequencing in cancer research: progress and perspectives

et al. 2021

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Single-cell sequencing, including genomics, transcriptomics, epigenomics, proteomics and metabolomics sequencing, is a powerful tool to decipher the cellular and molecular landscape at a single-cell resolution, unlike bulk sequencing, which provides averaged data. The use of single-cell sequencing in cancer research has revolutionized our understanding of the biological characteristics and dynamics within cancer lesions. In this review, we summarize emerging single-cell sequencing technologies and recent cancer research progress obtained by single-cell sequencing, including information related to the landscapes of malignant cells and immune cells, tumor heterogeneity, circulating tumor cells and the underlying mechanisms of tumor biological behaviors. Overall, the prospects of single-cell sequencing in facilitating diagnosis, targeted therapy and prognostic prediction among a spectrum of tumors are bright. In the near future, advances in single-cell sequencing will undoubtedly improve our understanding of the biological characteristics of tumors and highlight potential precise therapeutic targets for patients.

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Section: Applications Of Single-cell Sequencing In Cancer Biologymentioning

confidence: 99%

Applications of single-cell sequencing in cancer research: progress and perspectives

et al. 2021

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“…Experiment I: Mouse small intestine microbiota collection 17,18 . Three-week-old ICR male mice were dosed with ddH 2 O as the control or AOS 10 mg/kg BW via oral gavage (0.1 ml/mouse/d).…”

Section: Use Committee Of the Institute Of Animal Sciences Of Chinese Academy Ofmentioning

confidence: 99%

“…Very recently, it has been established that the gut microbiota plays crucial roles in spermatogenesis and male fertility [16][17][18][19] . Studies demonstrate a strong link between testicular function and the regulation of gut microbiota via host metabolomes, as beneficial microbiota have been shown to significantly improve busulfan impaired spermatogenesis and semen quality 17, 18 . Since noting the high incidence of infertility in T1D male patients, many investigators have tried to improve the semen quality and fertility in T1D induced animal models 20, 21 .…”

Section: Introductionmentioning

confidence: 99%

Gut-testis axis: microbiota-(n-3) PUFA improving semen quality in type 1 diabetes

Zhao

Hao

Feng

et al. 2021

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Gut dysbiosis and type 1 diabetes (T1D) are closely related, and gut dysbiosis and male infertility are correlated, too. Moreover, most male T1D patients are of active reproductive age. Therefore, it is crucial to explore possible means for improving their semen quality. Here, we found that fecal microbiota transplantation (FMT) from alginate oligosaccharide (AOS) improved gut microbiota (A10-FMT) significantly decreased blood glucose and glycogen, and increased semen quality in streptozotocin-induced T1D subjects. A10-FMT improved T1D-disturbed gut microbiota, especially the increase in small intestinal lactobacillus, and blood and testicular metabolome to produce n-3 polyunsaturated fatty acid (PUFA) docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) to ameliorate spermatogenesis and semen quality. Moreover, A10-FMT can improve spleen and liver function to strengthen the systemic environment for sperm development. FMT from gut microbiota of control animals (Con-FMT) produced some beneficial effects; however, to a smaller extent. Thus, AOS improved gut microbiota may be a useful protocol for improving semen quality and male fertility in T1D patients.ImportanceClinical data suggest that male reproductive dysfunction especially infertility is a critical issue for type 1 diabetic patient (T1D) because most of them are at the reproductive age. Gut dysbiosis is involved in T1D related male infertility. However, improved gut microbiota can be used to improve spermatogenesis and male fertility in T1D remains incompletely understood. We discovered that alginate oligosaccharide-improved gut microbiota (A10-FMT) significantly ameliorated spermatogenesis and semen quality. AOS-improved gut microbiota (specific microbes) may serve as a novel, promising therapeutic approach for the improvement of semen quality and male fertility in T1D patients.

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“…Recent studies also suggested that the gut microbiota changes could rescue male infertility following administration of alginate oligosaccharides (AOS). The authors explored the sperm quality and spermatogenesis in AOS-dosed animals after fecal microbiota transplantation into Busulfan-treated mice and found that healthy-body microbiota transplantation could improve spermatogenesis by altering bacterial species [36,37].…”

Section: Introductionmentioning

confidence: 99%

Chestnut Polysaccharides Rescue Impaired Spermatogenesis by Adjusting Gut Microbiota

Sun

Sheng

Tian

et al. 2021

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Background: Our previous study confirmed the beneficial effects of chestnut polysaccharides on the spermatogenesis process, but the exact mechanism is not clear. Several studies have demonstrated the importance of a balanced gut microbiota in maintaining normal reproductive function. In this study, we investigated the biological functions of chestnut polysaccharides from the perspective of gut microbiota function, expecting to elaborate the specific mechanism of chestnut polysaccharides beneficial to spermatogenesis.Results: Compared with the Vehicle group, germ cell quantity, intestinal structure and tight junction of intestinal tissue were altered in the Busulfan-treated mice, and the intestinal microbiota was also disturbed at several levels, including phylum and genus. The Firmicutes was predominant bacteria in all group followed by Proteobacteria, Bacteroidetes, Actinobacteria, Tenericutes, Cyanobacteria and unidentified-Bacteria, however, the composition of those gut microbiota changes caused by different treatment. Busulfan disturbed the homeostasis of the intestinal microenvironment. Chestnut polysaccharides could rescue this aberrance and improve spermatogenesis via steroid hormone synthesis process. Conclusions: Chestnut polysaccharides can change gut structure and tight junctions to rescue Busulfan-impaired spermatogenesis by altering the composition of different groups of gut microbes, a rescue process that is most likely related to hormone synthesis processes. It will provide a new insight for treating the male-related infertility.

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Rescue of male fertility following faecal microbiota transplantation from alginate oligosaccharide-dosed mice

Cited by 43 publications

References 9 publications

Applications of single-cell sequencing in cancer research: progress and perspectives

Applications of single-cell sequencing in cancer research: progress and perspectives

Gut-testis axis: microbiota-(n-3) PUFA improving semen quality in type 1 diabetes

Chestnut Polysaccharides Rescue Impaired Spermatogenesis by Adjusting Gut Microbiota

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