Requirements for T Lymphocyte Migration in Explanted Lymph Nodes

Huang, Julie H.; Cárdenas-Navia, Laura I.; Caldwell, Charles C.; Plumb, Troy J.; Radu, Caius G.; Rocha, Paulo Novis; Wilder, Tuere; Bromberg, Jonathan S.; Cronstein, Bruce N.; Sitkovsky, Michail V.; Dewhirst, Mark W.; Dustin, Michael L.

doi:10.4049/jimmunol.178.12.7747

Cited by 115 publications

(134 citation statements)

References 72 publications

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“…The in vivo measurements of cell migration and communication are also in good agreement with microscopy studies performed in situ in excised, intact murine LNs (Bousso and Robey 2003). However, such in situ imaging studies require careful control of the environment for temperature and supply with gases (Huang et al 2007). Furthermore, it is not clear whether the absence of innervation, lymph-, and blood-Xow aVects cellular functions at a more subtle level.…”

Section: Imaging Of Lymphoid Tissuessupporting

confidence: 62%

3D and 4D imaging of immune cells in vitro and in vivo

Nitschke

Garin

Kosco‐Vilbois

et al. 2008

Histochem Cell Biol

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Analyzing the dynamics of cellular immune responses, although performed for decades in immunologic research, has seen an enormous increase in the number of studies using this approach since the development of intravital 2-photon microscopy. Meanwhile, new insights into the dynamics of cellular immunity are being published on a daily basis. This review gives a short overview of the currently most widely used techniques, both on the microscopy side as well as on the experimental part. DiYculties and promises will be discussed. Finally, a personal selection of the most interesting Wndings of the Wrst 6 years of intravital 2-photon microscopy for immunological questions will be given. The overall aim is to get the reader interested into this fascinating way of investigating the immune response by means of "dynamic histology". This already has and will continue to broaden our view on how immune cells work in real life.

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Section: Imaging Of Lymphoid Tissuessupporting

confidence: 62%

3D and 4D imaging of immune cells in vitro and in vivo

Nitschke

Garin

Kosco‐Vilbois

et al. 2008

Histochem Cell Biol

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“…Oxygen electrode and hypoxia marker drug studies have demonstrated that the thymus of mice is heterogeneously hypoxic 6,15 , but in this tissue there is no evidence for HIF1-regulated gene expression 15 . Recently it was shown that lymphocyte motility in lymph nodes is affected by pO 2 , with motility decreasing with oxygen content 26 . Thus, hypoxia in the thymus might serve to keep maturing lymphocytes within the thymus until they are ready to be released.…”

Section: Regulation Of Hif1 By Hypoxiamentioning

confidence: 99%

Cycling hypoxia and free radicals regulate angiogenesis and radiotherapy response

2008

Self Cite

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Hypoxia and free radicals, such as reactive oxygen and nitrogen species, can alter the function and/or activity of the transcription factor hypoxia-inducible factor 1 (HIF1). Interplay between free radicals, hypoxia and HIF1 activity is complex and can influence the earliest stages of tumour development. The hypoxic environment of tumours is heterogeneous, both spatially and temporally, and can change in response to cytotoxic therapy. Free radicals created by hypoxia, hypoxia-reoxygenation cycling and immune cell infiltration after cytotoxic therapy strongly influence HIF1 activity. HIF1 can then promote endothelial and tumour cell survival. As discussed here, a constant theme emerges: inhibition of HIF1 activity will have therapeutic benefit.Virchow first described the abnormal structure of tumour vessels using contrast agents in human tumours as early as the mid 1800s 1 . A few decades later, Goldman was among the first to suggest that angiogenesis was associated with tumour growth 2 . A number of other investigators in the nineteenth and early twentieth centuries evaluated tumour angiogenesis, using techniques such as microangiography, vascular casting and window chamber models. Warren has reviewed this early work in great detail 3 . Folkman illuminated the crucial role of tumour angiogenesis by hypothesizing that tumour growth was dependent upon angiogenesis and that, if angiogenesis could be inhibited, it could maintain tumour deposits in a quiescent state 4 . These early works set the stage for the concept that tumours require angiogenesis to provide a nutrient supply. Although it is well-established that angiogenesis occurs in nearly all human solid tumours, it does not occur in an efficient manner, leading to spatial and temporal inadequacies in delivery of oxygen and other nutrients. These inefficiencies in nutrient delivery lead to a brutal cycle of unsatisfied demand by the tumour, which drives continued aberrant angiogenesis.The transcription factor hypoxia-inducible factor 1 (HIF1) plays an important part in tumour progression by upregulating genes that control angiogenesis, meta-stasis and resistance to oxidative stress. It also controls the switch to anaerobic metabolism, which can maintain cell NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript viability under hypoxic conditions. Further, HIF1 activity can promote treatment resistance by promoting endothelial and tumour cell survival after cytotoxic therapy. The mechanisms that control HIF1 activity are complex and are influenced by microenvironmental factors involving hypoxia, oxidative and nitrosative stress.In this Review we will discuss four important and interrelated aspects of tumour hypoxia. First, we will define the hypoxic response, discussing its relevance in influencing tumour cell survival, behaviour and angiogenesis. We will examine the physiological factors that contribute to hypoxia, emphasizing a perspective based on spatial and temporal dysfunction of tumour microcirculation. The question of whethe...

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“…7). This suggested that JAM-C indirectly affected lymphocyte trafficking by controlling the intranodal homeostatic chemokine content, which in turn regulated intranodal lymphocyte velocity and delayed naive T lymphocyte exit (21).…”

Section: Jam-c Controls Frc Dp Chemokine Secretion In Vitro and In Vivomentioning

confidence: 99%

Cutting Edge: JAM-C Controls Homeostatic Chemokine Secretion in Lymph Node Fibroblastic Reticular Cells Expressing Thrombomodulin

Frontera

Arcangeli

Zimmerli

et al. 2011

The Journal of Immunology

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The development and maintenance of secondary lymphoid organs, such as lymph nodes, occur in a highly coordinated manner involving lymphoid chemokine production by stromal cells. Although developmental pathways inducing lymphoid chemokine production during organogenesis are known, signals maintaining cytokine production in adults are still elusive. In this study, we show that thrombomodulin and platelet-derived growth factor receptor α identify a population of fibroblastic reticular cells in which chemokine secretion is controlled by JAM-C. We demonstrate that Jam-C–deficient mice and mice treated with Ab against JAM-C present significant decreases in stromal cell-derived factor 1α (CXCL12), CCL21, and CCL19 intranodal content. This effect is correlated with reduced naive T cell egress from lymph nodes of anti–JAM-C–treated mice.

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Requirements for T Lymphocyte Migration in Explanted Lymph Nodes

Cited by 115 publications

References 72 publications

3D and 4D imaging of immune cells in vitro and in vivo

3D and 4D imaging of immune cells in vitro and in vivo

Cycling hypoxia and free radicals regulate angiogenesis and radiotherapy response

Cutting Edge: JAM-C Controls Homeostatic Chemokine Secretion in Lymph Node Fibroblastic Reticular Cells Expressing Thrombomodulin

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