Requirement of T cells for the development of spontaneous autoimmune thyroiditis in obese strain (OS) chickens.

Objective. To investigate the potential of bone marrow cells from mice with primary antiphospholipid syndrome (APS) to transfer the disease to naive mice, and to determine the importance of the role of T cells in the APS. Methods. Experimental primary APS was induced in naive mice following active immunization with anticardiolipin (aCL) monoclonal antibody (MAb). Whole‐population or T cell–depleted bone marrow cells from mice with experimental primary APS were infused into total body–irradiated naive BALB/c recipients. Results. Bone marrow cells (in the presence of T cells) had the potential to induce experimental APS in naive mice, which resulted in high serum titers of aCL, antiphosphatidylserine, and antiphosphatidylinositol antibodies; an increased number of antibody‐forming cells specific for each of the above phospholipids; a positive lymph node cell proliferative response to aCL MAb; and clinical features of primary APS, including thrombocytopenia, prolonged activated partial thromboplastin time (indicating the presence of lupus anticoagulant), and a high frequency of fetal resorptions (the equivalent of human fetal loss). T cell–depleted bone marrow cells did not transfer the disease. Conclusion. This study demonstrates the important role of T cells in the development and transfer of experimental primary APS and raises the possibility of T cell manipulations in treatments to prevent this condition.

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MHC‐ and non‐MHC‐encoded surface antigens of chicken lymphoid cells and erythrocytes recognized by polyclonal xeno‐, allo‐ and monoclonal antibodies

Wolf

Hála

Boyd

et al. 1984

Eur J Immunol

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Surface antigens on chicken thymus and bursa cells were analyzed by immunoprecipitation using polyclonal and monoclonal antisera raised against (and specific for) thymus (ATS) or bursa (ABS) cells, respectively. The antigens identified were compared with those governed by the B-F, B-L and B-G regions of the chicken major histocompatibility complex (B complex). Four proteins were precipitated from thymus cells by 2 polyclonal ATS: both antisera recognized molecules of apparent molecular mass of 172-182, 132-135, 75-76 kDa, and one antiserum in addition recognized a protein of 102 kDa. The 172-182 and 102-kDa peaks were still demonstrable under reducing conditions indicating that they are composed of a single polypeptide chain, the other 2 were lost under reducing conditions, therefore, must be composed of smaller subunits. Of the 2 monoclonal ATS tested, one identified a single protein of 186 kDa and the other a 135-kDa protein (in addition to 2 smaller molecules); whether these are the same as those precipitated by the polyclonal antisera remains to be determined as they behaved differently under reducing conditions. Proteins of 162 and 78-84 kDa were revealed by 2 polyclonal ABS under nonreducing conditions but the former may in one case be a polymer (it disappeared under reducing conditions) and in the other a single molecule. In addition molecules of 182 kDa were identified by one antiserum and of 84 and 60 kDa by the other under nonreducing conditions. Of the 4 monoclonal ABS only one identified a 200-kDa protein: molecules of 115-125, 90-100, 48-52 and 40-43 kDa were also precipitated, all of which were reduced to smaller molecules. With 2 specific anti-B-F alloantisera we were able to precipitate the "conventional" B-F antigen from red blood cell lysates of CB-strain chickens resolving into a 40-kDa peak and a light chain of about 12 kDa corresponding to beta 2 microglobulin. Precipitates from peripheral blood lymphocytes, bursa and thymus cells revealed an additional protein of 22 kDa. With 2 specific B-L alloantisera two peaks of 33 kDa and 31 kDa were obtained from peripheral blood lymphocytes. Using anti-B-G alloantisera a double band corresponding to 47 and 42 kDa was seen under reducing conditions. There is no evidence from these data to indicate that the polyclonal and monoclonal antibodies are directed towards major histocompatibility complex antigens.

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Requirement of T cells for the development of spontaneous autoimmune thyroiditis in obese strain (OS) chickens.

Cited by 27 publications

References 0 publications

The Immunological Basis of Autoimmune Disease

The Immunological Basis of Autoimmune Disease

Transfer of experimental antiphospholipid syndrome by bone marrow cell transplantation the importance of the t cell

MHC‐ and non‐MHC‐encoded surface antigens of chicken lymphoid cells and erythrocytes recognized by polyclonal xeno‐, allo‐ and monoclonal antibodies

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