Optical 3R regeneration is of great interest due to its ability to enhance the system margins in photonic network, because it enables to reset transmission impairments induced by channel interaction distortion, accumulation of amplified spontaneous emission (ASE) noise, chromatic dispersion and non-linearities of optical fibers and so forth. In comparison to electrical 3R regenerators, optical 3R regenerators are expected to be less complex and require less highspeed electronic components owing to simple optical signal processing, leading to small footprint, low power consumption and low cost. In this paper, we describe an optical 3R regenerator operable with carrier-suppressed returnto-zero (CS-RZ) modulation format which will be employed in dense WDM (DWDM) network to increase the efficiency of bandwidth utilization. We have demonstrated 3R regenerative transmission using 100 GHz-spacing 40 Gbit/s 5 WDM CS-RZ signals so as to confirm that a DWDM filtering effect can be drastically mitigated by the 3R regenerator.