Abstract:Cholesterol is essential for neuronal activity and function. Cholesterol depletion in the plasma membrane impairs synaptic transmission. However, the molecular mechanisms by which cholesterol deficiency leads to defects in vesicle fusion remain poorly understood. Here we show that cholesterol is required for Ca2+-dependent fusion using the in-vitro reconstitution of vesicle fusion, atomic force microscopy (AFM), and amperometry to monitor exocytosis in chromaffin cells. Purified native vesicles were crucial fo… Show more
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