Requirement for Tyrosine Phosphatase during Serotonergic Neuromodulation by Protein Kinase C

Catarsi, Stefano; Drapeau, Pierre

doi:10.1523/jneurosci.17-15-05792.1997

Cited by 20 publications

(19 citation statements)

References 35 publications

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“…This kind of shape could originate from two distinct mechanisms: first, receptor desensitization occurring at higher agonist concentrations (Lopez-Garcia and King 1996; Wilkie et al 1996) and second, the existence of two different types of 5-HT receptors, as in leech mechanosensory P cells (Drapeau and Sanchez-Armass 1988;Henderson 1983) and S cells (Burrell et al 2001). In leech P cells, application of low levels of 5-HT onto the soma produces an increase in Cl Ϫ conductance and a consequent hyperpolarization, possibly mediated by one type of 5-HT 3 receptor (Ali et al 1998), whereas a higher level of 5-HT induces depolarization resulting from the activation of one type of 5-HT 2 receptor causing an increase of a nonspecific cationic conductance (Sanchez-Armass et al 1991;Catarsi and Drapeau 1997). Ionic mechanisms for 5-HT-mediated inhibition and excitation are unknown in the S cell but could be explained by different 5-HT receptors and receptor desensitization (Burrell et al 2001).…”

Section: Resultsmentioning

confidence: 99%

Statistical characterization of social interactions and collective behavior in medicinal leeches

Bisson

Torre

2011

Journal of Neurophysiology

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Bisson G, Torre V. Statistical characterization of social interactions and collective behavior in medicinal leeches. J Neurophysiol 106: 78 -90, 2011. First published March 16, 2011 doi:10.1152/jn.01043.2010In the present study we analyzed the behavior and interactions among leeches in the same observation tank. Colored beads were glued onto their skin so that their behavior could be followed and quantified. When two or three leeches were present in the observation tank, they searched around for a maximum of 2 h and their motion and behavior were independent from those of their conspecifics. When the number of leeches in the tank was increased to 10, leeches were attracted to each other and exhibited episodes of highly correlated behavior. Solitary leeches injected with serotonin or dopamine increased the portion of time spent pseudoswimming and crawling, respectively. The behavior of three to five leeches injected with serotonin was not statistically independent, and leeches were attracted to their conspecifics and exhibited episodes of correlated behavior. Therefore, serotonin not only induces pseudoswimming in leeches but also promotes social interactions, characterized by a mutual attraction and by episodes of correlated/collective behavior.

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Section: Resultsmentioning

confidence: 99%

Statistical characterization of social interactions and collective behavior in medicinal leeches

Bisson

Torre

2011

Journal of Neurophysiology

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“…If this also occurs in the P cell, we hypothesize that dopaminergic signalling may occur by activation of a macromolecular complex containing the receptor, PKA and the channel. Interestingly, a non-synaptic cation channel in the P cell is thought to be tightly associated with a tyrosine phosphatase (Catarsi & Drapeau, 1997) and tyrosine phosphatases are also thought to be tightly associated with Aplysia bag cell neuron cation channels (Wilson & Kaczmarek, 1993) and NMDA receptors in mammalian neurons (Wang, Yu & Salter, 1996). These observations raise the interesting possibility that second messenger signalling may commonly occur in macromolecular assemblies that include the channel as an efficient means for spatial and temporal regulation of channel activity.…”

Section: Discussionmentioning

confidence: 99%

Ionotropic and metabotropic activation of a neuronal chloride channel by serotonin and dopamine in the leech Hirudo medicinalis

Ali

Catarsi

Drapeau

1998

The Journal of Physiology

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Cl− channels on the pressure‐sensitive (P) neuron in the leech are directly activated by synaptic release of serotonin (5‐HT) and are indirectly stimulated by the cAMP second messenger pathway, suggesting an unusual dual regulation of the channels. We have investigated the mode of action of 5‐HT and dopamine (DA) on a Cl− channel in adult P cells in culture by recording from cell‐attached patches. 5‐HT increased Cl− channel activity only when included in the recording pipette and not when applied in the bath. Pipette or, more effectively, bath application of DA led to an increase in Cl− channel activity. This effect was blocked by the potent and specific dopaminergic (DA1) receptor blocker, SCH‐23390. The stimulation by DA, but not by 5‐HT, was also blocked by the cAMP‐dependent protein kinase A (PKA) inhibitor Rp‐cAMP and was mimicked by the membrane‐permeant cAMP analogue dibutyryl cAMP (db‐cAMP). Our results show that 5‐HT directly gates a Cl− channel that is also activated by DA via the cAMP pathway. This study demonstrates that a ligand‐gated channel can be independently operated by another transmitter acting via a second messenger pathway.

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“…In the leech P-cells, application of low levels of 5HT onto the soma produces hyperpolarization of the membrane potential that may be mediated by a 5HT 3 receptor (Ali et al, 1998), whereas more 5HT induces depolarization resulting from the activation of a 5HT 2 -like receptor (Catarsi and Drapeau, 1997). The two 5HT receptor populations appear to be differentially distributed between the synaptic and extrasynaptic regions of the P-cell.…”

Section: Dual Effects Are Mediated By Different 5ht Receptorsmentioning

confidence: 98%

Non-Associative Learning and Serotonin Induce Similar Bi-Directional Changes in Excitability of a Neuron Critical for Learning in the Medicinal Leech

2001

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In studies of the cellular basis of learning, much attention has focused on plasticity in synaptic transmission in terms of transmitter release and the number or responsiveness of neurotransmitter receptors. However, changes in postsynaptic excitability independent of receptors may also play an important role. Changes in excitability of a single interneuron in the leech, the S-cell, were measured during non-associative learning of the whole-body shortening reflex. This interneuron was chosen because it is known to be necessary for sensitization and full dishabituation of the shortening response. During sensitization, S-cell excitability increased, and this enhancement corresponded to facilitation of the shortening reflex and increased S-cell activity during the elicited response. During habituation training, there was a decrement in both the shortening reflex and the elicited S-cell activity, along with decreased S-cell excitability. Conversely, dishabituation facilitated both the shortening response and S-cell activity during shortening, with an accompanying increase in S-cell excitability. Bath application of 1-10 M serotonin (5HT), a modulatory neurotransmitter that is critical for sensitization, for full dishabituation, and for associative learning, increased S-cell excitability. S-cell excitability also increased after stimulation of the serotonergic Retzius cells. However, focal application of serotonin onto the S-cell soma hyperpolarized the interneuron, and bath application of a lower dose of serotonin (0.1 M) decreased excitability. The observed changes in postsynaptic excitability appear to contribute to non-associative learning, and modulatory neurotransmitters, such as serotonin, evidently help regulate excitability. Such changes in S-cell excitability may also be relevant for more complex, associative forms of learning.

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Requirement for Tyrosine Phosphatase during Serotonergic Neuromodulation by Protein Kinase C

Cited by 20 publications

References 35 publications

Statistical characterization of social interactions and collective behavior in medicinal leeches

Statistical characterization of social interactions and collective behavior in medicinal leeches

Ionotropic and metabotropic activation of a neuronal chloride channel by serotonin and dopamine in the leech Hirudo medicinalis

Non-Associative Learning and Serotonin Induce Similar Bi-Directional Changes in Excitability of a Neuron Critical for Learning in the Medicinal Leech

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