Requirement for particular seed‐borne fungi for seed germination and seedling growth of <i>Xyris complanata</i>, a pioneer monocot in topsoil‐lost tropical peatland in Central Kalimantan, Indonesia

Hashidoko, Yasuyuki; Ogita, Noriko; Limin, Suwido H.; Tahara, Satoshi

doi:10.1007/s11284-007-0411-y

Cited by 18 publications

(12 citation statements)

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“…In addition, it has been found that Penicillium spp. promote seed germination, as well as seedling growth, and even protect seedlings of Picea glehnii (Yamaji et al 2001) and Xyris complanata (Tamura et al 2008). We now have evidence that Phoma sp., T. koningii and P. chrysogenum break mechanical but not physiological seed dormancy, and promote seed germination in O. streptacantha.…”

Section: Discussionmentioning

confidence: 77%

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Are fungi important for breaking seed dormancy in desert species? Experimental evidence in Opuntia streptacantha (Cactaceae)

et al. 2010

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Seeds of Opuntia spp. have physiological dormancy; they need a period of after-ripening to break dormancy, and the embryos have low growth potential. We evaluated the combined effects of seed age and presence of fungi on the testa on germination of Opuntia streptacantha, an abundant species in the Chihuahuan Desert (Mexico), assuming that older seeds have broken seed dormancy and fungi can reduce mechanical resistance to germination. In a preliminary experiment, we found no germination of 9-year-old (1998) and freshly collected (2007) seeds. However, we obtained 67% and 27% germination from 9-year-old and fresh non-sterilized seeds, respectively, and found fungi growing on the testa of all germinated seeds. Two fungal strains were isolated and identified using ribosomal internal transcribed spacer (ITS) sequence analysis: Penicillium chrysogenum and Phoma sp. In a second experiment, we inoculated seeds with strains of P. chrysogenum and Phoma sp., as well as Trichoderma koningii and binucleate Rhizoctonia (Gto17S2), to evaluate their ability to break seed dormancy. Seeds inoculated with P. chrysogenum, Phoma sp. and T. koningii had higher germination than controls for both seed ages, but germination was higher in older seeds. Scanning electron microscopy showed that these fungi eroded the funiculus, reducing its resistance. Binucleate Rhizoctonia did not lead to germination and controls had almost no germination. Our results strongly indicate that fungi are involved in breaking seed dormancy of O. streptacantha, and that the effect of fungi on seeds is species-specific.

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Section: Discussionmentioning

confidence: 77%

“…promote seed germination, as well as seedling growth, and even protect seedlings of Picea glehnii (Yamaji et al. 2001) and Xyris complanata (Tamura et al. 2008).…”

Section: Discussionmentioning

confidence: 99%

Are fungi important for breaking seed dormancy in desert species? Experimental evidence in Opuntia streptacantha (Cactaceae)

et al. 2010

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“…DNA was extracted from the disrupted mycelia using an Isoplant II DNA kit (Nippon Gene, Toyama, Japan). Using the resulting DNA as the template, the ITS region was amplified by PCR using a pair of universal primers (forward ITS 1, 5=-TCCGTAGGTGAACCTGCGG-3=; and reverse ITS 4, 5=-TCCTCCGCTTATTGATATGC-3=) as reported before (22). The PCR amplicon (600 bp) was sequenced using a BigDye Terminator v 3.1 cycle sequencing kit (Applied Biosystems, Tokyo, Japan) with primer ITS 4 according to the protocol recommended for an ABI Prism 310 genetic analyzer (Applied Biosystems, CA).…”

Section: Methodsmentioning

confidence: 99%

Carot-4-en-9,10-Diol, a Conidiation-Inducing Sesquiterpene Diol Produced by Trichoderma virens PS1-7 upon Exposure to Chemical Stress from Highly Active Iron Chelators

Wang

Hashimoto

Hashidoko

2013

Appl Environ Microbiol

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To screen biocontrol agents against Burkholderia plantarii, the causative agent of rice seedling blight, we employed catechol, an analog of the virulence factor tropolone, to obtain chemical stress-resistant microorganisms. The fungal isolate PS1-7, identified as a strain of Trichoderma virens, showed the highest resistance to catechol (20 mM) and exhibited efficacy as a biocontrol agent for rice seedling blight. During investigation of metabolic traits of T. virens PS1-7 exposed to catechol, we found a secondary metabolite that was released extracellularly and uniquely accumulated in the culture. The compound induced by chemical stress due to catechol was subsequently isolated and identified as a sesquiterpene diol, carot-4-en-9,10-diol, based on spectroscopic analyses. T. virens PS1-7 produced carot-4-en-9,10-diol as a metabolic response to tropolone at concentrations from 0.05 to 0.2 mM, and the response was enhanced in a dose-dependent manner, similar to its response to catechol at concentrations from 0.1 to 1 mM. Some iron chelators, such as pyrogallol, gallic acid, salicylic acid, and citric acid, at 0.5 mM also showed activation of T. virens PS1-7 production of carot-4-en-9,10-diol. This sesquiterpene diol, formed in response to chemical stress, promoted conidiation of T. virens PS1-7, suggesting that it is involved in an autoregulatory signaling system. In a bioassay of the metabolic and morphological responses of T. virens PS1-7, conidiation in hyphae grown on potato dextrose agar (PDA) plates was either promoted or induced by carot-4-en-9,10-diol. Carot-4-en-9,10-diol can thus be regarded as an autoregulatory signal in T. virens, and our findings demonstrate that intrinsic intracellular signaling regulates conidiation of T. virens.

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“…With those isolates that had sporulated, inoculum was prepared by adding 10 ml sterile distilled water to a colony, scraping conidia from the agar surface with a sterile scalpel, and adjusting the concentration to approximately 5 × 10 6 conidia/ml with a hemocytometer. For fungal isolates that failed to sporulate, mycelial suspensions were prepared as follows (Tamura et al, 2008). Fungal isolates were cultured with shaking in 50-ml potato dextrose broth medium at 22°C for several days, and the resulting mycelium was homogenized with a blender (Nippon Seiki, Nagaoka, Japan) after removal of the culture medium.…”

Section: Pathogenicity Testsmentioning

confidence: 99%

Grazing Intensity Affects Communities of Culturable Root‐Associated Fungi in a Semiarid Grassland of Northwest China

et al. 2017

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Intensive grazing by large herbivores is known to result in the degeneration of grassland worldwide. However, little is known about how grazing affects the fungi living in roots, especially those with septate hyphae. We investigated grazing intensity effects on culturable root‐associated fungi (c‐RAF) of three dominant plant species; Artemisia capillaris, Lespedeza davurica, and Stipa bungeana, in a semiarid grassland of northwest China. Plant roots were sampled from four grazing intensities at four sampling dates. The total colonization rate of c‐RAF was not affected by grazing intensity, but the diversity of c‐RAF displayed a hump‐shaped trend with grazing intensities. Few c‐RAF were present in all plant species, with most of them being confined to only one or two plant species. The 16 most commonly isolated species were selected to investigate their effects on plant growth and health. Three Fusarium species were most pathogenic c‐RAF. Three dark‐sterile species did not affect growth parameters, regardless of host plants. With A. capillaris, the occurrence of Fusarium spp. was highest in the control plots, while with S. bungeana, the occurrence tended to increase as the grazing intensity increased. Two dark‐sterile species, including Darksidea alpha, in L. davurica roots increased with grazing intensities; in contrast, D. alpha decreased for S. bungeana with grazing intensities. Our results strongly suggest that in grasslands, grazing intensity can lead to changes in the colonization of specific root fungal taxa, and these fungal changes would potentially feed back to plant performance depending on the abundance of host plants. Copyright © 2017 John Wiley & Sons, Ltd.

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Requirement for particular seed‐borne fungi for seed germination and seedling growth of Xyris complanata, a pioneer monocot in topsoil‐lost tropical peatland in Central Kalimantan, Indonesia

Cited by 18 publications

References 28 publications

Are fungi important for breaking seed dormancy in desert species? Experimental evidence in Opuntia streptacantha (Cactaceae)

Are fungi important for breaking seed dormancy in desert species? Experimental evidence in Opuntia streptacantha (Cactaceae)

Carot-4-en-9,10-Diol, a Conidiation-Inducing Sesquiterpene Diol Produced by Trichoderma virens PS1-7 upon Exposure to Chemical Stress from Highly Active Iron Chelators

Grazing Intensity Affects Communities of Culturable Root‐Associated Fungi in a Semiarid Grassland of Northwest China

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