Requirement for Par-6 and Bazooka in<i>Drosophila</i>border cell migration

Pinheiro, Elaine M.; Montell, Denise J.

doi:10.1242/dev.01412

Cited by 123 publications

(156 citation statements)

References 26 publications

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“…3L) in the mutant cells respectively. Combined with the previous reports that both aPKC/Bazooka (Baz) complex and DE-Cadherin are required for the collective migration of border cell cluster, 3,6,15,16,44,45 results in this work suggest that Lgl functions in maintaining the cluster integrity through mediating the cell polarity and cell-cell adhesion. To test this, we overexpressed Baz in lgl 4 mutant border cells based on MARCM technique, and observed a significantly reduced penetrance of the dissociated border cell cluster, albeit the phenotype was not fully restored (Fig.…”

Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tsupporting

confidence: 84%

“…[5][6][7][8][9][10][11][12][13][14] In addition, the apico-basal polarity and DE-Cadherin based adhesion are crucial for the integrity of border cell cluster during its morphogenetic movement. 6,15,16 As developmental cell migration and pathological cell invasion involve common cellular and biological processes, 3,10,[17][18][19][20][21] further investigation of this model system will contribute to unraveling the molecular mechanisms underlying cancer invasion and metastasis. The Drosophila nTSGs lgl, dlg and scrib cooperatively function in multiple biological processes, including cell polarity and proliferation control in epithelial tissues, tumor cell invasion and asymmetric division of neuroblasts.…”

Section: Introductionmentioning

confidence: 99%

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Requirements of Lgl in cell differentiation and motility during Drosophila ovarian follicular epithelium morphogenesis

Li¹,

Feng²,

Yu³

et al. 2011

Fly

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Section: ©2 0 1 1 L a N D E S B I O S C I E N C E D O N O T D I S Tsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Requirements of Lgl in cell differentiation and motility during Drosophila ovarian follicular epithelium morphogenesis

Li¹,

Feng²,

Yu³

et al. 2011

Fly

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“…Interestingly, this context-specific function for Scribble may represent a more common theme for polarity proteins in migration (Humbert et al, 2006). In the Drosophila ovary, loss of Bazooka/Par3 or Par6 function in border cell epithelial migration results in disorganization of the border cell cluster and impaired migration (Pinheiro and Montell, 2004). In contrast, loss of Bazooka/Par3 in the context of activated Ras in the eye imaginal disc epithelium can give rise to invasive tumours in Drosophila (Pagliarini and Xu, 2003).…”

Section: Conserved Role For Scribble In Cell Migrationmentioning

confidence: 99%

The tumour-suppressor Scribble dictates cell polarity during directed epithelial migration: regulation of Rho GTPase recruitment to the leading edge

Dow

Kauffman

Caddy³

et al. 2006

Oncogene

161

143

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Altered expression of human Scribble is associated with invasive epithelial cancers, however, its role in tumour development remains unclear. Mutations in Drosophila Scribble result in loss of polarity, overproliferation and 3D-tumourous overgrowth of epithelial cells. Using complementation studies in Drosophila we recently demonstrated that expression of human Scribble can also regulate polarity and restrict tissue overgrowth. Here, we have undertaken a detailed study of human Scribble function in the polarized mammary cell line, MCF10A. We show that although Scribble does not seem to be required for apical-basal polarity or proliferation control in MCF10A cells, Scribble is essential for the control of polarity associated with directed epithelial cell migration. Scribble-depleted MCF10A cells show defective in vitro wound closure and chemotactic movement. The cells at the wound edge fail to polarize, show reduced lamellipodia formation and impaired recruitment of Cdc42 and Rac1 to the leading edge. Furthermore, we show that this function is relevant in vivo as Scribble mutant mice show defective epidermal wound healing. This data identifies an essential role for mammalian Scribble in the regulation of the polarity specifically involved in directed epithelial migration.

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“…6C; Spearman correlation ϭ 0.89; P ϭ 0.0002; see Experimental Procedures section), suggesting that BLJs actively suppress motility pathways to prevent tumor invasion. Indeed, loss of function of proteins from the other four epithelial junctions either had no impact or delayed or blocked BC migration (data not shown) (Peifer et al, 1993;Niewiadomska et al, 1999;Zarnescu and Thomas, 1999;AbdelilahSeyfried et al, 2003;Pinheiro and Montell, 2004), further indicating that repression of motility is a unique function of the BLJ. To obtain further insight into how BLJs control invasion, we expressed a dominant-negative allele of Rac1, a G-protein essential for BC migration (Geisbrecht and Montell, 2004), in Fas2 and dlg epithelium.…”

Section: Derepression Of Motility Pathways Is Necessary But Not Suffimentioning

confidence: 95%

“…Thus, the exclusion of apical and basal domains, and the predominance of the lateral membrane, may create a unique targeting niche essential for motility. BLJ-specific docking motifs in vesicular proteins such as SNARE (Pinheiro and Montell, 2004) may be important for rapid membrane cycling in moving cells (Mellman, 2000). Acquisition of the lateralized niche appears to be precisely timed in BCs, but is quasi-stochastic in BLJ mutant tumors (Fig.…”

Section: Bljs Are Sufficient To Suppress Epithelial Invasionmentioning

confidence: 99%

Basolateral junctions are sufficient to suppress epithelial invasion during Drosophila oogenesis

Szafrański

Goode

2006

Developmental Dynamics

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Epithelial junctions play crucial roles during metazoan evolution and development by facilitating tissue formation, maintenance, and function. Little is known about the role of distinct types of junctions in controlling epithelial transformations leading to invasion of neighboring tissues. Discovering the key junction complexes that control these processes and how they function may also provide mechanistic insight into carcinoma cell invasion. Here, using the Drosophila ovary as a model, we show that four proteins of the basolateral junction (BLJ), Fasciclin-2, Neuroglian, Discs-large, and Lethal-giant-larvae, but not proteins of other epithelial junctions, directly suppress epithelial tumorigenesis and invasion. Remarkably, the expression pattern of Fasciclin-2 predicts which cells will invade. We compared the apicobasal polarity of BLJ tumor cells to border cells (BCs), an epithelium-derived cluster that normally migrates during mid-oogenesis. Both tumor cells and BCs differentiate a lateralized membrane pattern that is necessary but not sufficient for invasion. Independent of lateralization, derepression of motility pathways is also necessary, as indicated by a strong linear correlation between faster BC migration and an increased incidence of tumor invasion. However, without membrane lateralization, derepression of motility pathways is also not sufficient for invasion. Our results demonstrate that spatiotemporal patterns of basolateral junction activity directly suppress epithelial invasion by organizing the cooperative activity of distinct polarity and motility pathways. Developmental Dynamics 236:364 -373, 2007.

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Requirement for Par-6 and Bazooka inDrosophilaborder cell migration

Cited by 123 publications

References 26 publications

Requirements of Lgl in cell differentiation and motility during Drosophila ovarian follicular epithelium morphogenesis

Requirements of Lgl in cell differentiation and motility during Drosophila ovarian follicular epithelium morphogenesis

The tumour-suppressor Scribble dictates cell polarity during directed epithelial migration: regulation of Rho GTPase recruitment to the leading edge

Basolateral junctions are sufficient to suppress epithelial invasion during Drosophila oogenesis

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