Requirement for Mass Spectrometry Sex Steroid Assays in the Journal of Clinical Endocrinology and Metabolism

Handelsman, David J.; Wartofsky, Léonard

doi:10.1210/jc.2013-3375

Cited by 311 publications

(197 citation statements)

References 33 publications

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“…Other previous studies have reported stable serum E 2 levels with regard to male aging using conventional RIAs (38,39,40,46) or GC-MS (43,47,48), whereas studies using direct immunoassays reported no change (49, 50) or a decline (51). Some of these discrepancies are likely due to the unreliability of E 2 immunoassays at the low circulating levels prevailing in men (24,25,26,52 The mechanism and significance of the biochemical decline in androgen status affecting aging men cannot be determined from the present observational study. Nevertheless, an important and plausible explanation is that this decline reflects the impact of the co-morbidities accumulating during aging on an otherwise healthy reproductive system.…”

Section: Discussioncontrasting

confidence: 65%

Age-specific population centiles for androgen status in men

Handelsman

Yeap

Flicker

et al. 2015

European Journal of Endocrinology

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Aim: The age-specific population profiles in men of circulating testosterone and its two bioactive metabolites dihydrotestosterone (DHT) and estradiol (E 2 ) across the adult lifespan and its determinants are not well described. Objective: Our objective was to deduce smoothed age-specific centiles of circulating testosterone, DHT, and E 2 in men using pooled data from population-based studies in three Australian cities from liquid chromatography-mass spectrometry steroid measurements in a single laboratory. Design, setting, and participants: We pooled data of 10 904 serum samples (serum testosterone, DHT, E 2 , age, height, and weight) from observational population-based studies in three major cities across Australia. Main outcome measures: Age-specific smoothed centiles for serum testosterone, DHT, and E 2 in men aged 35-100 years were deduced by large sample data analysis methods. Results: We found that serum testosterone, DHT, and E 2 decline gradually from ages 35 onwards with a more marked decline after 80 years of age. Higher weight, BMI, and body surface area as well as shorter stature are associated with reduced serum testosterone, DHT, and E 2 . Conclusions: Among Australian men, there is a gradual progressive population-wide decline in androgen status during male aging until the age of 80 years after which there is a more marked decline. Obesity and short stature are associated with reduced androgen status. Research into the age-related decline in androgen status should focus on the progressive accumulation of age-related comorbidities to better inform optimal clinical trial design.

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Section: Discussioncontrasting

confidence: 65%

Age-specific population centiles for androgen status in men

Handelsman

Yeap

Flicker

et al. 2015

European Journal of Endocrinology

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“…Also, many now propose that steroids should no longer be measured by inaccurate immunoassays and that steroid assays should be replaced by mass spectrometric methods, which are highly specific and sensitive, while providing a full spectrum of steroid measurements in a single sample in one assay [29]. Although standard methods for steroid profiling from urine or plasma are known, they are only established in few laboratories and normative data are scarce.…”

Section: Discussionmentioning

confidence: 99%

Estimation of reference curves for the urinary steroid metabolome in the first year of life in healthy children: Tracing the complexity of human postnatal steroidogenesis

Dhayat

et al. 2015

The Journal of Steroid Biochemistry and Molecular Biology

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Estimation of reference curves for the urinary steroid metabolome in the first year of life in healthy children: tracing the complexity of human postnatal steroidogenesis, Journal of Steroid Biochemistry and Molecular Biology http://dx.doi.org/10. 1016/j.jsbmb.2015.07.024 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.Page 1 of 24 Page 2 of 24 Graphical abstract HighlightsThe steroid metabolome during fetal-neonatal transition varies with sex and postnatal age.For 67 urinary steroid compounds normative values have been quantified by GC-MS for the first year of life.Highest concentrations are found for progesterone metabolites and glucocorticoids.In both sexes, most compounds peak at week 3 and decrease to 20% by week 25.Normative data for the urine steroid metabolome provide a powerful diagnostic tool. AbstractContext: Complex steroid disorders such as P450 oxidoreductase deficiency or apparent cortisone reductase deficiency may be recognized by steroid profiling using chromatographic mass spectrometric methods. These methods are highly specific and sensitive, and provide a complete spectrum of steroid metabolites in a single measurement of one sample which makes them superior to immunoassays. The steroid metabolome during the fetal-neonatal transition is characterized by a) the metabolites of the fetal-placental unit at birth, b) the fetal adrenal androgens until its involution 3-6 months postnatally, and c) the steroid metabolites produced by the developing endocrine organs. All these developmental events change the steroid metabolome in an age-and sex-dependent manner during the first year of life.Objective: The aim of this study was to provide normative values for the urinary steroid metabolome of healthy newborns at short time intervals in the first year of life. Methods:We conducted a prospective, longitudinal study to measure 67 urinary steroid metabolites in 21 male and 22 female term healthy newborn infants at 13 time-points from week 1 to week 49 of life. Urine samples were collected from newborn infants before discharge from hospital and from healthy infants at home. Steroid metabolites were measured by gas chromatography-mass spectrometry (GC-MS) and steroid concentrations corrected for urinary creatinine excretion were calculated.Results: 61 steroids showed age and 15 steroids sex specificity. Highest urinary steroid concentrations were found in both sexes for progesterone derivatives, in particular 20α-DH-5α-DH-progesterone, and for highly polar 6α-hydroxylated glucocorticoids. The steroids peaked at week 3 and decreased by ~80% at week 25 in both sexes. The decline of progestins, andro...

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“…They include cross reactivity with similar analytes, standardisation issues between labs and sensitivity issues (7). It is now recognised that performance of some IA techniques may be suboptimal, this recently prompted a recommendation from the editors of Journal of Clinical Endocrinology and Metabolism to avoid using such assays and instead use MS for the measurement of sex steroids (8). This sparked a vigorous debate which resulted in the Endocrine Society Council establishing a 'Sex Steroid Assays Reporting Task Force'.…”

Section: -Franz Kafkamentioning

confidence: 99%

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

Taylor¹,

Keevil²,

Huhtaniemi³

2015

European Journal of Endocrinology

254

157

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SummaryThe recent onslaught of mass spectrometry (MS) to measurements of steroid hormones, including demands that they should be the only acceptable method, has confused clinicians and scientists who have relied for more than 40 years on a variety of immunoassay (IA) methods in steroid hormone measurements. There is little doubt that MS methods with their superior specificity will be the future method of choice in many clinical and research applications of steroid hormone measurement. However, the majority of steroid measurements are currently, and will continue to be, carried out using various types of IAs for several reasons, including their technical ease, cost and availability of commercial reagents. Speedy replacement of all IAs with MS is an unrealistic and unnecessary goal, because the availability of MS measurements is limited by cost, need of expensive equipment, technical demands and lack of commercial applications. Furthermore, IAs have multiple well-known advantages that vindicate their continuing use. The purpose of this article is to elucidate the advantages and limitations of the MS and IA techniques from two angles, i.e. promotion of MS and defence of IA. The purpose of the text is to give the reader an unbiased view about the current state and future trends of steroid analysis and to help him/her choose the correct assay method to serve his/her diagnostic and research needs.

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Requirement for Mass Spectrometry Sex Steroid Assays in the Journal of Clinical Endocrinology and Metabolism

Cited by 311 publications

References 33 publications

Age-specific population centiles for androgen status in men

Age-specific population centiles for androgen status in men

Estimation of reference curves for the urinary steroid metabolome in the first year of life in healthy children: Tracing the complexity of human postnatal steroidogenesis

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

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