Requirement for glucose during in vitro culture of sheep preimplantation embryos

Thompson, Jeremy G.; Simpson, A. C.; Pugh, P.A.; Tervit, H.R.

doi:10.1002/mrd.1080310405

Cited by 107 publications

(49 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, it is known that during pre-blastocyst stages of embryonic development, utilisation of glucose as an energy source is not possible and that only the stages from blastocysts onwards can benefit from increased glucose concentrations (Leese and Barton 1984;Thompson et al 1992). Increased glycolysis in the endometria of heifers with a retarded embryo would therefore not be beneficial but its actual function and relevance in the uterine endometrium is unknown (Gao et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Characterisation of endometrial gene expression and metabolic parameters in beef heifers yielding viable or non-viable embryos on Day 7 after insemination

Beltman

Forde

Furney

et al. 2010

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

Abridged title: Endometrial gene expression and metabolic parameters in subfertile beef heifers. AbstractThe objective of this study was to compare the hormonal, metabolic characteristics and endometrial gene expression profiles in beef heifers yielding either a viable or a degenerate embryo on Day 7 after insemination as a means to explain differences in embryo survival.Oestrus was synchronised in cross bred beef heifers (n=145) using a CIDR-prostaglandin protocol and heifers (n=102) detected in standing oestrus (within 24-48 h post CIDR removal) were inseminated 12-18 h after detection of oestrus (Day 0) with frozen-thawed semen from a single ejaculate of a bull with proven fertility. Blood samples were collected from Day 4 to Day 7 post oestrus to measure progesterone (on Days 4, 5 and 7), insulin and IGF-I (on Days 4 and 6) and urea concentrations (on Day 7). All animals were slaughtered on Day 7. Uterine pH was also determined. Animals from which an embryo was recovered were classified as either having a viable embryo (morula/blastocyst stage); n=32, or a retarded embryo (arrested at 2-to 16-cell stage); n=19. The overall recovery rate was 64%. There was no significant difference in blood parameters measured or uterine pH at slaughter between the heifers with either a viable or retarded embryo. The relative abundance of 9 transcripts out of 53 tested from the endometrial tissue was different between heifers with a viable embryo or retarded embryo: MOGAT1, PFKB2, LYZ2, SVS8, UHRF1, PTGES, AGPAT4, DGKA and HGPD. Both LYZ2 and UHRF1 are associated with regulation of the immune system. PFKFB2 is a mediator in glycolysis and MOGAT, AGPAT4 and DGKA belong to the triglyceride synthesis pathway. PTGES and HGPD belong to the prostaglandin pathway.Both of these metabolic pathways are important for early embryonic development. In conclusion, retarded embryo development in this study was not related to serum progesterone, IGF-I, insulin or urea concentrations nor to uterine pH at slaughter. However, altered expression of genes involved in the prostaglandin and triglyceride pathways, as well as 2 genes that are closely associated with the regulation of immunity in the endometrium may indicate a uterine component to the retardation of the embryos in these beef heifers.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterisation of endometrial gene expression and metabolic parameters in beef heifers yielding viable or non-viable embryos on Day 7 after insemination

Beltman

Forde

Furney

et al. 2010

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

show abstract

“…In most other species, excessive medium glucose is detrimental to embryo development (Schini & Bavister 1988, Seshagiri & Bavister 1991, Takahashi & First 1992, Thompson et al 1992, Conaghan et al 1993. The early cleavage-stage embryo may be especially sensitive to the effects of glucose, because the metabolism of embryos of many species changes from a reliance on oxidative phosphorylation of lactate or pyruvate (which may be inhibited by excess glycolytic activity) during the early cleavage stages to glycolysis at the compactedmorula stage and into blastocyst development as metabolic requirements increase to support growth and differentiation (Rieger et al 1992, Gardner 1998.…”

Section: Introductionmentioning

confidence: 99%

Cell lineage allocation in equine blastocysts produced in vitro under varying glucose concentrations

et al. 2015

View full text Add to dashboard Cite

Equine embryos develop in vitro in the presence of high glucose concentrations, but little is known about their requirements for development. We evaluated the effect of glucose concentrations in medium on blastocyst development after ICSI. In experiment 1, there were no significant differences in rates of blastocyst formation among embryos cultured in our standard medium (DMEM/F-12), which contained O16 mM glucose, and those cultured in a minimal-glucose embryo culture medium (!1 mM; Global medium, GB), with either 0 added glucose for the first 5 days, then 20 mM (0-20) or 20 mM for the entire culture period . In experiment 2, there were no significant differences in the rates of blastocyst development (31-46%) for embryos cultured in four glucose treatments in GB (0-10, 0-20, 5-10, or 5-20). Blastocysts were evaluated by immunofluorescence for lineage-specific markers. All cells stained positively for POU5F1. An inner cluster of cells was identified that included presumptive primitive endoderm cells (GATA6-positive) and presumptive epiblast (EPI) cells. The 5-20 treatment resulted in a significantly lower number of presumptive EPI-lineage cells than the 0-20 treatment did. GATA6-positive cells appeared to be allocated to the primitive endoderm independent of the formation of an inner cell mass, as was previously hypothesized for equine embryos. These data demonstrate that equine blastocyst development is not dependent on high glucose concentrations during early culture; rather, environmental glucose may affect cell allocation. They also present the first analysis of cell lineage allocation in in vitro-fertilized equine blastocysts. These findings expand our understanding of the factors that affect embryo development in the horse.

show abstract

“…Glucose is widely used as a major energy substrate in embryo culture media. Although exposure to high concentrations of glucose during early embryonic stages causes developmental retardation in many species (Thompson et al 1992, Conaghan et al 1993, Kim et al 1993, and a two-cell block in mouse and hamster embryos (Schini & Bavister 1988, Lawitts & Biggers 1991, glucose is known to be an important energy substrate for blastocyst formation in the post-compaction period of bovine embryos (Rieger et al 1992). This dramatic diversity of the effect of glucose is related to the switch of the energy metabolism pathway in embryos.…”

Section: Discussionmentioning

confidence: 99%

The proteasomal inhibitor MG132 increases the efficiency of mouse embryo production after cloning by electrofusion

Yu¹,

Yong²,

Li³

et al. 2005

Reproduction

View full text Add to dashboard Cite

In this study, we cloned mice from ES cells by a post-electrofusion MG132 treatment and improved development of cloned embryos with a sequential cultivation protocol. When 5 mM MG132, a proteasome inhibitor, were used to treat the reconstructed embryos, the capacity of in vitro development, implantation and full-term development were significantly improved. Blastocyst formation rates of the reconstructed embryos from X4 ES cells (F1 strain derived from C57BL/6 3 129sv) and J1 ES cells obtained with or without MG132 treatment were 66.9% and 26.6%, and 66.1% and 34.5% respectively (P < 0.05). A total of 146 two-cell embryos cloned from X4 ES cells with MG132 treatment were transferred to recipients, and five cloned pups (3.4%) were born, of which four survived. When the same numbers of two-cell embryos cloned from X4 ES cells without MG132 treatment were transferred, however, no live-born mice were obtained. When embryos cloned from J1 ES cells without MG132 treatment were cultured in KSOM medium for 54 h followed by culture in CZB medium containing 5.6 mM glucose for 42 h, the blastocyst rate was significantly higher than when they were cultured in KSOM continuously for 96 h (34.5% vs 17.1%). However, sequential cultivation did not improve the development of embryos cloned with MG132 treatment and that of parthenotes. In conclusion, MG132 treatment increased the developmental potential of reconstructed mouse embryos, and sequential cultivation improved development of the embryos cloned by electrofusion without MG132 treatment.

show abstract

Requirement for glucose during in vitro culture of sheep preimplantation embryos

Cited by 107 publications

References 18 publications

Characterisation of endometrial gene expression and metabolic parameters in beef heifers yielding viable or non-viable embryos on Day 7 after insemination

Characterisation of endometrial gene expression and metabolic parameters in beef heifers yielding viable or non-viable embryos on Day 7 after insemination

Cell lineage allocation in equine blastocysts produced in vitro under varying glucose concentrations

The proteasomal inhibitor MG132 increases the efficiency of mouse embryo production after cloning by electrofusion

Contact Info

Product

Resources

About