Requirement for fos gene expression in the transcriptional activation of collagenase by other oncogenes and phorbol esters

Schönthal, Axel H.; Herrlich, Peter; Rahmsdorf, Hans J.; Ponta, Helmut

doi:10.1016/0092-8674(88)90195-x

Cited by 580 publications

(304 citation statements)

References 58 publications

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“…We observed an 18-fold enhancement of HMGIC promoter activity ( Figure 8f). This activation by p21Ras is in the same range as observed with a Fos promoter construct (Schonthal et al, 1988) which was included as positive control.…”

Section: Characterization Of the Hmgic Promotersupporting

confidence: 52%

Regulation of HMGIC expression: an architectural transcription factor involved in growth control and development

Jansen

et al. 1999

Oncogene

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The HMGIC gene has been implicated in the control of cell proliferation and development. We show here that HMGIC has multiple mRNA isoforms that arise by transcription initiation from alternative tandem promoters. These transcripts are not only di erentially expressed between cell lines, but they can also di er within an individual cell line, in response to particular stimuli. Whereas quiescent 3T3-L1 preadipocytes express low levels of HMGIC mRNA, stimulation by serum results in a dramatic upregulation with the characteristics of a delayed-early response gene. Characterization of involved signal transduction pathways showed that both FGF-1 and PDGF-BB are strong inducers of HMGIC expression mediated via both the PI-3 kinase and MAP kinase pathways. In order to characterize the regulatory elements, sequences upstream of the translation initiation site of HMGIC were assayed for promoter activity. The HMGIC 5'¯anking sequences had constitutive promoter activity in all cell lines tested, suggesting that HMGIC is regulated by negative regulatory elements that were not present in the 5'-anking regions analysed here.

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Section: Characterization Of the Hmgic Promotersupporting

confidence: 52%

Regulation of HMGIC expression: an architectural transcription factor involved in growth control and development

Jansen

et al. 1999

Oncogene

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“…The v-src-mediated repression of c-fos induction occurs at the transcriptional level suggesting that prolonged oncogenic Ras expression could also inhibit c-fos transcription. Expression of c-fos following growth factor treatment of normal ®broblasts is transient and is stringently turned o (Lin et al, 1988;SchoÈ nthal et al, 1988). The mechanism of c-fos downregulation is complex and involves di erent transcription factors including the cFos product itself (Sassone-Corsi et al, 1988b;SchoÈ nthal et al, 1989).…”

Section: Discussionmentioning

confidence: 99%

Transformation by ras modifies AP1 composition and activity

et al. 1997

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The Ras proteins play a central role in regulating cell growth and their mutation can lead to abnormal proliferation. To analyse the potential link betwen AP1 activity, encoded by members of the jun and fos gene families, and Ras-mediated cellular transformation, we have studied several NIH3T3 clones which overexpress the Ha-Ras or Ki-Ras oncogenes. These transformed ®broblasts accumulated higher levels of cJun, JunB, Fra1 and Fra2 proteins relative to their normal counterparts. They also displayed increased AP1 DNA binding activity which was predominantly composed of cJun and Fra1 containing dimers. Following serum stimulation of Ras clones, the elevated levels of cJun and Fra1 remained steady, while the induction of JunB and Fra2 was partially attenuated. Moreover, deregulated Ras signaling resulted in a complete loss of the serum inducibility of cFos and FosB. Ectopic co-expression of cJun and Fra1 in NIH3T3 ®broblasts led to a transformed phenotype, attenuation of cFos serum inducibility, increased AP1 activity and Cyclin D1 accumulation, all characteristics of oncogenic Ras expressing cells. These results demonstrate that cJun and Fra1 are crucial mediators of the Ras-transformation process.

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“…Examples of proteins expressed are type 1 collagenase and stromelysin 1, both of which are proteases that are involved in the degradation of the ECM (Kerr et al, 1988;Schonthal et al, 1988). In order to identify more AP-1 regulated genes that are needed for FBR invasion, we have used di erential screening and subtractive suppression hybridization (Diatchenko et al, 1996;Hennigan et al, 1994;Johnston et al, 1999, unpublished).…”

Section: Introductionmentioning

confidence: 99%

Krp1, a novel kelch related protein that is involved in pseudopod elongation in transformed cells

et al. 2000

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Requirement for fos gene expression in the transcriptional activation of collagenase by other oncogenes and phorbol esters

Cited by 580 publications

References 58 publications

Regulation of HMGIC expression: an architectural transcription factor involved in growth control and development

Regulation of HMGIC expression: an architectural transcription factor involved in growth control and development

Transformation by ras modifies AP1 composition and activity

Krp1, a novel kelch related protein that is involved in pseudopod elongation in transformed cells

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