Requirement for expression of WW domain containing transcription regulator 1 in bovine trophectoderm development

Saito, Shoichiro; Yamamura, Shota; Kohri, Nanami; Bai, Hanako; Takahashi, Masashi; Kawahara, Manabu

doi:10.1016/j.bbrc.2021.03.112

Cited by 7 publications

(4 citation statements)

References 27 publications

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“…The BNC are likely most abundant in the cotyledon, as the hormones they produce have greater access to the maternal circulation given that uterine blood flow is preferentially directed to the caruncles. A cluster of nuclei adjacent to BNCs in both tissues expressed WWTR1 , a gene involved with the YAP pathway which regulates maintenance of cytotrophoblast-like fate and essentially blocking the transition to a syncytiotrophoblast in humans ( 37 , 63 , 64 ). Cell trajectory analysis of trophoblasts predicted a framework for UNC to BNC differentiation ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Single-nuclei RNA sequencing (snRNA-seq) uncovers trophoblast cell types and lineages in the mature bovine placenta

Davenport

Ortega

Liu

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Ruminants have a semi-invasive placenta, which possess highly vascularized placentomes formed by maternal endometrial caruncles and fetal placental cotyledons and required for fetal development to term. The synepitheliochorial placenta of cattle contains at least two trophoblast cell populations, including uninucleate (UNC) and binucleate (BNC) cells that are most abundant in the cotyledonary chorion of the placentomes. The interplacentomal placenta is more epitheliochorial in nature with the chorion developing specialized areolae over the openings of uterine glands. Of note, the cell types in the placenta and cellular and molecular mechanisms governing trophoblast differentiation and function are little understood in ruminants. To fill this knowledge gap, the cotyledonary and intercotyledonary areas of the mature day 195 bovine placenta were analyzed by single nuclei analysis. Single-nuclei RNA-seq analysis found substantial differences in cell type composition and transcriptional profiles between the two distinct regions of the placenta. Based on clustering and cell marker gene expression, five different trophoblast cell types were identified in the chorion, including proliferating and differentiating UNC and two different types of BNC in the cotyledon. Cell trajectory analyses provided a framework for understanding the differentiation of trophoblast UNC into BNC. The upstream transcription factor binding analysis of differentially expressed genes identified a candidate set of regulator factors and genes regulating trophoblast differentiation. This foundational information is useful to discover essential biological pathways underpinning the development and function of the bovine placenta.

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Section: Discussionmentioning

confidence: 99%

Single-nuclei RNA sequencing (snRNA-seq) uncovers trophoblast cell types and lineages in the mature bovine placenta

Davenport

Ortega

Liu

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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“…Both BNC populations express binucleate-specific PAGs ( PAG3 , PAG16 , and PAG17 ) in addition to CSH2 . The BNC 1 and 2 populations are distinguished by the expression of WWTR1 , a gene involved with the YAP pathway and trophectoderm lineage specification in the preimplantation bovine embryo in addition to the regulation of cytotrophoblast trophoblast fate in the human placenta [60, 61]. The two different BNC populations and separate lineages were previously characterized from single-nuclei RNA sequencing of the mature bovine placenta [35].…”

Section: Discussionmentioning

confidence: 99%

Single-cell insights into development of the bovine placenta

Davenport,

O’Neil,

Ortega

et al. 2023

Biology of Reproduction

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A central determinant of pregnancy success is proper development of the conceptus (embryo/fetus and associated extraembryonic membranes including the placenta). Although the gross morphology and histology of the bovine placenta has been well studied, the cellular and molecular mechanisms regulating placenta development and trophoblast differentiation and function remain essentially undefined. Here single cell transcriptome (scRNA-seq) analysis was performed on the day 17 bovine conceptus and chorion of day 24, 30 and 50 conceptuses (n = 3–4 samples per day) using the 10X Genomics platform. Bioinformatic analyses identified cell types and their ontogeny including trophoblast, mesenchyme, and immune cells. Loss of IFNT expressing trophoblast uninucleate cells (UNC) occurred between days 17 and 30, whereas binucleate cells (BNC), identified based on expression of placental lactogen (CSH2) and specific pregnancy-associated glycoproteins (PAGs) genes, first appeared on day 24. Several different types of UNC were present in day 24, 30 and 50 samples, but only one (day 24) or two types of BNC (days 30 and 50). Cell trajectory analyses provided a conceptual framework for UNC development and BNC differentiation, and bioinformatic analyses identified candidate transcription factors governing differentiation and function of the trophoblasts. The digital atlas of cell types in the developing bovine conceptus reported here serves as a resource to discover key genes and biological pathways regulating its development during the critical periods of implantation and placentation.

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“…Bovine oocyte retrieval, IVM, IVF, and subsequent IVC up to the blastocyst stage were performed as previously described [ 23 ]. Briefly, cumulus-oocyte complexes (COCs) collected from slaughterhouse-derived Holstein ovaries were cultured in TCM-199 medium (Thermo Fisher Scientific Inc., Waltham, MA, USA) at 38.5°C in a humidified atmosphere of 5% CO 2 for 20–22 h. In vitro -matured oocytes were transferred to Brackett and Oliphant (B.O.)…”

Section: Methodsmentioning

confidence: 99%

Generation of viable calves derived from developmentally mature blastocysts produced by on-gel culture

Saito

Akizawa

Furukawa

et al. 2022

Journal of Reproduction and Development

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Conventional culture systems for bovine embryos are unable to support sustained embryonic development until the developmentally mature blastocyst stage. Although we have previously developed an on-gel culture system that enables bovine blastocysts to complete cell segregation events at day (D) 10 following in vitro culture, the development of D10 blastocysts to term has yet to be achieved. In this study, we attained full-term development of D10 mature blastocysts produced using an on-gel culture system. Two calves derived from on-gel-cultured embryos were vaginally born, showing normal birth and placental weights and no obvious morphological abnormalities. Moreover, we detected no abnormalities in blood metabolic profile analyses. Our findings indicate that on-gel culturing can be used to facilitate the development of developmentally mature blastocysts to term, and produce healthy viable calves. This culture system could make a valuable contribution to cattle production and would enable a range of analyses for characterizing bovine-specific pre-implantation development.

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Requirement for expression of WW domain containing transcription regulator 1 in bovine trophectoderm development

Cited by 7 publications

References 27 publications

Single-nuclei RNA sequencing (snRNA-seq) uncovers trophoblast cell types and lineages in the mature bovine placenta

Single-nuclei RNA sequencing (snRNA-seq) uncovers trophoblast cell types and lineages in the mature bovine placenta

Single-cell insights into development of the bovine placenta

Generation of viable calves derived from developmentally mature blastocysts produced by on-gel culture

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