Repurposing Plant Virus Nanoparticles

Hefferon, Kathleen

doi:10.3390/vaccines6010011

Cited by 31 publications

(21 citation statements)

References 61 publications

(78 reference statements)

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“…Their repetitive organization and immunogenicity are ideal prerequisites for new types of safe and potent vaccines. These are accessible from plants within 2–3 weeks and thus already in phase III clinical trials, with allowances in veterinary as well as emergency uses in humans (reviewed for example, in Balke & Zeltins, ; Hefferon, ; K. L.Lee, Twyman, Fiering, & Steinmetz, ; Lomonossoff & D'Aoust, ; Rybicki, ; Takeyama, Kiyono, & Yuki, ). In the pipeline of precision diagnostics and pharmaceuticals, plant VNPs double‐ or oligofunctionalized with imaging agents, disease‐ or cell type‐specific targeting ligands, and therapeutics might push the boundaries of present drugs, as indicated by many proof‐of‐concept studies.…”

Section: Introductionmentioning

confidence: 99%

From stars to stripes: RNA‐directed shaping of plant viral protein templates—structural synthetic virology for smart biohybrid nanostructures

Wege

Koch

2019

WIREs Nanomed Nanobiotechnol

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The self‐assembly of viral building blocks bears exciting prospects for fabricating new types of bionanoparticles with multivalent protein shells. These enable a spatially controlled immobilization of functionalities at highest surface densities—an increasing demand worldwide for applications from vaccination to tissue engineering, biocatalysis, and sensing. Certain plant viruses hold particular promise because they are sustainably available, biodegradable, nonpathogenic for mammals, and amenable to in vitro self‐organization of virus‐like particles. This offers great opportunities for their redesign into novel “green” carrier systems by spatial and structural synthetic biology approaches, as worked out here for the robust nanotubular tobacco mosaic virus (TMV) as prime example. Natural TMV of 300 x 18 nm is built from more than 2,100 identical coat proteins (CPs) helically arranged around a 6,395 nucleotides ssRNA. In vitro, TMV‐like particles (TLPs) may self‐assemble also from modified CPs and RNAs if the latter contain an Origin of Assembly structure, which initiates a bidirectional encapsidation. By way of tailored RNA, the process can be reprogrammed to yield uncommon shapes such as branched nanoobjects. The nonsymmetric mechanism also proceeds on 3'‐terminally immobilized RNA and can integrate distinct CP types in blends or serially. Other emerging plant virus‐deduced systems include the usually isometric cowpea chlorotic mottle virus (CCMV) with further strikingly altered structures up to “cherrybombs” with protruding nucleic acids. Cartoon strips and pictorial descriptions of major RNA‐based strategies induct the reader into a rare field of nanoconstruction that can give rise to utile soft‐matter architectures for complex tasks. This article is categorized under: Biology‐Inspired Nanomaterials > Protein and Virus‐Based Structures Nanotechnology Approaches to Biology > Nanoscale Systems in Biology Biology‐Inspired Nanomaterials > Nucleic Acid‐Based Structures

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Section: Introductionmentioning

confidence: 99%

From stars to stripes: RNA‐directed shaping of plant viral protein templates—structural synthetic virology for smart biohybrid nanostructures

Wege

Koch

2019

WIREs Nanomed Nanobiotechnol

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“…Immunogenic Epitope peptides can be displayed on the surface capsid protein subsequently those peptides assembled like virus particles act as vehicles for epitope display. Expression of immunogenic epitope peptides as fusions with surface loops of plant viral Capsid proteins has been used for the generation of several candidate animal vaccines (Hefferon 2018).…”

Section: Discussionmentioning

confidence: 99%

Molecular Modelling and Insilico Engineering of PapMV-CP Towards Display and Development of Capripox Viral Like Particles Based on Immunogenic P32 Envelop Protein is the Homologous of the Vaccinia-Viral H3L Gene: An Insilico Approach

Kumar

Reddy

et al. 2020

Int J Pept Res Ther

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Viral-like particles are assembled from capsid protein structural subunits of different viruses and have ability to establish research in biomedicals, like construction of novel safety vaccines, gene therapy vectors by delivering systems for nucleic acids, small biomolecules and diagnostics. Papaya Mosaic Viral nanoparticals can provide as a vaccine candidate helps to increase the immunity by fusing the epitope based peptide antigen. Capripox viruses are the genus comprises Lymphy skin-disease, Sheep and Goat pox Viruses are notified by The World Animal Health Organization (OIE) based on their economic impotence act as a transboundary animal diseases viruses of sheep, goat, and cattle's respectively. Plant viral based innovative vaccines have been emerged ineffective vaccine development. This research describes the engineering and development of a new vaccine candidate by display immunogenic peptide using the carrier capsid protein of Papaya Mosaic Virus. The Capripox virus P32 immunogenic protein is homologous of the vaccinia virus H3L gene displayed PapMV CP. The antigenicity of P32 protein epitope lowest score among epitopes C-terminally docked epitopes are EP6 > EP3 > EP8 as well the lowest score among epitopes N-terminally docked epitopes are EP8 > EP3 > EP6 presented on the N-terminus of PMV CP region which are found to be suitable for epitope display. And these modelled immunogenic peptide could be used to develop a viral like particles. Epitope based Antibody developed against immunogenic epitopic regions can contribute to a novel and robust protection from infection. As well might be used for developing cost effective detection kits for Transboundary animal disease viruses. Keywords Viral-like particles • Capripox viruses • Papaya mosaic viral capsid protein • B-Cell epitopes Abbreviations VLPS Viral-like particles PMV CP Papaya mosaic virus capsid protein LSDV Lymphy skin-disease virus SPPV Sheeppox virus GPPV Goatpox virus EPs Epitopes

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“…Microbial nanotechnology is a bio-driven scientific discipline that interconnects microbial biotechnology and nanotechnology. Numerous biological resources have been exploited for the biosynthesis of nanoparticles, including bacteria [ 66 ], fungi [ 67 ], algae [ 68 ], viruses [ 69 ], plant extracts [ 70 ], and agro-industrial wastes [ 71 ]. Fungi, yeast, and bacteria are currently receiving particular attention for the biological production of nanoparticles owing to their capacity to biotransform and bioaccumulate metals ( Table 1 ).…”

Section: Microbial Synthesis Of Qdsmentioning

confidence: 99%

Superior Properties and Biomedical Applications of Microorganism-Derived Fluorescent Quantum Dots

et al. 2020

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Quantum dots (QDs) are fluorescent nanocrystals with superb photo-physical properties. Applications of QDs have been exponentially increased during the past decade. They can be employed in several disciplines, including biological, optical, biomedical, engineering, and energy applications. This review highlights the structural composition and distinctive features of QDs, such as resistance to photo-bleaching, wide range of excitations, and size-dependent light emission features. Physical and chemical preparation of QDs have prominent downsides, including high costs, regeneration of hazardous byproducts, and use of external noxious chemicals for capping and stabilization purposes. To eliminate the demerits of these methods, an emphasis on the latest progress of microbial synthesis of QDs by bacteria, yeast, and fungi is introduced. Some of the biomedical applications of QDs are overviewed as well, such as tumor and microRNA detection, drug delivery, photodynamic therapy, and microbial labeling. Challenges facing the microbial fabrication of QDs are discussed with the future prospects to fully maximize the yield of QDs by elucidating the key enzymes intermediating the nucleation and growth of QDs. Exploration of the distribution and mode of action of QDs is required to promote their biomedical applications.

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Repurposing Plant Virus Nanoparticles

Cited by 31 publications

References 61 publications

From stars to stripes: RNA‐directed shaping of plant viral protein templates—structural synthetic virology for smart biohybrid nanostructures

From stars to stripes: RNA‐directed shaping of plant viral protein templates—structural synthetic virology for smart biohybrid nanostructures

Molecular Modelling and Insilico Engineering of PapMV-CP Towards Display and Development of Capripox Viral Like Particles Based on Immunogenic P32 Envelop Protein is the Homologous of the Vaccinia-Viral H3L Gene: An Insilico Approach

Superior Properties and Biomedical Applications of Microorganism-Derived Fluorescent Quantum Dots

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