Reptilian myotomal myogenesis—lessons from the sand lizard Lacerta agilis L. (Reptilia, Lacertidae)

Rupik, Weronika; Swadzba, E.; Dubińska–Magiera, Magda; Jędrzejowska, Izabela; Daczewska, Małgorzata

doi:10.1016/j.zool.2012.04.002

Cited by 15 publications

(21 citation statements)

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“…These model vertebrate species provided detailed information at the morphological and the molecular level of muscle development in this phylum. Studies on reptilian developmental biology including myogenesis are difficult and rarely attempted (Nagashima et al 2005 ; Rupik et al 2012 ; Kusumi et al 2013 ). To better understand the mechanisms of trunk muscle differentiation and growth in reptiles, we chose the Egyptian cobra.…”

Section: Discussionmentioning

confidence: 99%

“…It was found that in the sand lizard, similarly to birds and mammals, the dermomyotome is the main source of muscle Pax3-positive progenitor cells. Studies conducted by Rupik et al ( 2012 ) revealed that muscle growth in the sand lizard is due to Pax3/Pax7-positive mononucleated cells. In comparison to the sand lizard, snakes, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Among all vertebrates, reptile myogenesis and myotomal muscle growth are poorly known. Studies on reptile myogenesis have been conducted on several species (Chinese soft-shelled turtle Pelodiscus sinensis and sand lizard Lacerta agilis ); among them, only sand lizard studies on myogenesis shed light on the origin of muscle progenitor cells and growth of myotomal muscles (Nagashima et al 2005 ; Rupik et al 2012 ). It was found that in the sand lizard, similarly to birds and mammals, the dermomyotome is the main source of muscle Pax3-positive progenitor cells.…”

Section: Introductionmentioning

confidence: 99%

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Unique features of myogenesis in Egyptian cobra (Naja haje) (Squamata: Serpentes: Elapidae)

et al. 2015

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During early stages of myotomal myogenesis, the myotome of Egyptian cobra (Naja haje) is composed of homogenous populations of mononucleated primary myotubes. At later developmental phase, primary myotubes are accompanied by closely adhering mononucleated cells. Based on localization and morphology, we assume that mononucleated cells share features with satellite cells involved in muscle growth. An indirect morphological evidence of the fusion of mononucleated cells with myotubes is the presence of numerous vesicles in the subsarcolemmal region of myotubes adjacent to mononucleated cell. As differentiation proceeded, secondary muscle fibres appeared with considerably smaller diameter as compared to primary muscle fibre. Studies on N. haje myotomal myogenesis revealed some unique features of muscle differentiation. TEM analysis showed in the N. haje myotomes two classes of muscle fibres. The first class was characterized by typical for fast muscle fibres regular distribution of myofibrils which fill the whole volume of muscle fibre sarcoplasm. White muscle fibres in studied species were a prominent group of muscles in the myotome. The second class showed tightly paced myofibrils surrounding the centrally located nucleus accompanied by numerous vesicles of different diameter. The sarcoplasm of these cells was characterized by numerous lipid droplets. Based on morphological features, we believe that muscle capable of lipid storage belong to slow muscle fibres and the presence of lipid droplets in the sarcoplasm of these muscles during myogenesis might be a crucial adaptive mechanisms for subsequent hibernation in adults. This phenomenon was, for the first time, described in studies on N. haje myogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Unique features of myogenesis in Egyptian cobra (Naja haje) (Squamata: Serpentes: Elapidae)

et al. 2015

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“…Material was dehydrated and embedded in an Epoxy Embedding Medium Kit (Sigma, St. Louis, MO). This procedure of fixation appears to be the best for different embryonic reptilian tissues (Rupik, Kowalska, Swadźba, & Maślak, ; Rupik, Swadźba, Dubińska‐Magiera, Jędrzejowska, & Daczewska, ; Swadźba & Rupik, ). Using a Leica Ultracut UCT microtome (Leica Microsystems, Wetzlar, Germany) semithin and ultrathin sections were cut.…”

Section: Methodsmentioning

confidence: 99%

“…Material was dehydrated and embedded in an Epoxy Embedding Medium Kit (Sigma, St. Louis, MO). This procedure of fixation appears to be the best for different embryonic reptilian tissues (Rupik, Kowalska, Swadźba, & Ma slak, 2016;Rupik, Swadźba, Dubi nska-Magiera, Jędrzejowska, & Daczewska, 2012;.…”

Section: Light and Transmission Electron Microscopymentioning

confidence: 99%

Development of pancreatic acini in embryos of the grass snake Natrix natrix (Lepidosauria, Serpentes)

Kowalska

Rupik

2019

Journal of Morphology

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This study report about the differentiation of pancreatic acinar tissue in grass snake, Natrix natrix, embryos using light microscopy, transmission electron microscopy, and immuno‐gold labeling. Differentiation of acinar cells in the embryonic pancreas of the grass snake is similar to that of other amniotes. Pancreatic acini occurred for the first time at Stage VIII, which is the midpoint of embryonic development. Two pattern of acinar cell differentiation were observed. The first involved formation of zymogen granules followed by cell migration from ducts. In the second, one zymogen granule was formed at the end of acinar cell differentiation. During embryonic development in the pancreatic acini of N. natrix, five types of zymogen granules were established, which correlated with the degree of their maturation and condensation. Within differentiating acini of the studied species, three types of cells were present: acinar, centroacinar, and endocrine cells. The origin of acinar cells as well as centroacinar cells in the pancreas of the studied species was the pancreatic ducts, which is similar as in other vertebrates. In the differentiating pancreatic acini of N. natrix, intermediate cells were not present. It may be related to the lack of transdifferentiation activity of acinar cells in the studied species. Amylase activity of exocrine pancreas was detected only at the end of embryonic development, which may be related to animal feeding after hatching from external sources that are rich in carbohydrates and presence of digestive enzymes in the egg yolk. Mitotic division of acinar cells was the main mechanism of expansion of acinar tissue during pancreas differentiation in the grass snake embryos.

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Comparative development of limb musculature in phylogenetically and ecologically divergent lizards

Díaz

Taylor-Diaz

Trainor

et al. 2021

Developmental Dynamics

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Background Squamate reptiles (lizards, snakes, and amphisbaenians) exhibit incredible diversity in their locomotion, behavior, morphology, and ecological breadth. Although they often are used as models of locomotor diversity, surprisingly little attention has been given to muscle development in squamate reptiles. In fact, the most detailed examination was conducted almost 80 years ago and solely focused on the proximal limb regions. Herein, we present forelimb and hindlimb muscle morphogenesis data for three lizard species with different locomotion and feeding strategies: the desert grassland whiptail lizard, the central bearded dragon, and the veiled chameleon. This study fills critical gaps in our understanding of muscle morphogenesis in squamate reptiles and presents a comparative and temporospatial analysis of muscle development. Results Our results reveal a conserved pattern of early muscle development among lizards with different adult morphologies and ecologies. The variations that exist are concentrated in distal regions, particularly the specialized autopodia of chameleons, where differentiation of muscles associated with the digits is delayed. Conclusions The chameleon autopod provides an example of major evolutionary modifications to the skeleton with only minor disruption of the conserved order and pattern of limb muscle development. This robustness of muscle patterning facilitates the evolution of extreme yet functional phenotypes.

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Reptilian myotomal myogenesis—lessons from the sand lizard Lacerta agilis L. (Reptilia, Lacertidae)

Cited by 15 publications

References 50 publications

Unique features of myogenesis in Egyptian cobra (Naja haje) (Squamata: Serpentes: Elapidae)

Unique features of myogenesis in Egyptian cobra (Naja haje) (Squamata: Serpentes: Elapidae)

Development of pancreatic acini in embryos of the grass snake Natrix natrix (Lepidosauria, Serpentes)

Comparative development of limb musculature in phylogenetically and ecologically divergent lizards

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