Reptiles as a Source of Salmonella O48—Clinically Important Bacteria for Children: The Relationship Between Resistance to Normal Cord Serum and Outer Membrane Protein Patterns

Bugla-Płoskońska, Gabriela; Korzeniowska‐Kowal, Agnieszka; Guz-Regner, Katarzyna

doi:10.1007/s00248-010-9677-7

Cited by 11 publications

(9 citation statements)

References 44 publications

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“…The isolation of OMPs was performed according to Murphy and Bartos [ 39 ] with own minor modifications [ 24 ]. Bacterial strains were cultured overnight at 37°C in 25 ml LB medium.…”

Section: Methodsmentioning

confidence: 99%

“…Enteritidis, can inhibit the formation of MAC by binding to factor 6, which is a part of the complement cascade system [ 23 ]. Bugla-Płoskońska et al confirmed that there are certain OMPs characteristic for the serum-sensitive and serum-resistant phenotypes of Salmonella O48 strains [ 24 ]. A number of previous studies have investigated the OMPs profiles of different Salmonella strains [ 25 – 28 ], but only one study on outer membrane proteins of S .…”

Section: Introductionmentioning

confidence: 99%

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Proteomic Analysis of Outer Membrane Proteins from Salmonella Enteritidis Strains with Different Sensitivity to Human Serum

et al. 2016

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Differential analysis of outer membrane composition of S. Enteritidis strains, resistant to 50% normal human serum (NHS) was performed in order to find factors influencing the resistance to higher concentrations of NHS. Ten S. Enteritidis clinical strains, resistant to 50% NHS, all producing very long lipopolysaccharide, were subjected to the challenge of 75% NHS. Five extreme strains: two resistant and three sensitive to 75% NHS, were chosen for the further analysis of outer membrane proteins composition. Substantial differences were found in the levels of particular outer membrane proteins between resistant and sensitive strains, i.e. outer membrane protease E (PgtE) was present mainly in resistant strains, while sensitive strains possessed a high level of flagellar hook-associated protein 2 (FliD) and significantly higher levels of outer membrane protein A (OmpA).

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“…The isolation of OMPs was performed according to Murphy and Bartos [ 39 ] with own minor modifications [ 24 ]. Bacterial strains were cultured overnight at 37°C in 25 ml LB medium.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Outer Membrane Proteins from Salmonella Enteritidis Strains with Different Sensitivity to Human Serum

et al. 2016

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“…These data raise the question as to whether released OMPs, such as the 18-kDa OMP, could play a role in the pathogenesis of Gram-negative sepsis. References: Bugla-Ploskonska et al (2011, 2010a, 2010b, 2009b, 2009cFutoma et al (2005); Sarowska et al (2010) NHS -normal human serum; NCS -normal cord serum; NBS -normal bovine serum 1 OMPs characteristic for only serum-sensitive strains (not present in serum-resistant strains) or for only serum-resistant strains (not present in serum-sensitive strains) 2 NT -not tested 3 data presented only in this paper 4 there are any individual OMPs in this strain when compare to others 5 strains were regarded as resistant when their survival was above 100% in the highest concentrations of serum Table 2. The susceptibility of Salmonella strains to NHS, NCS, and NBS put together with OMPs…”

Section: Susceptibility Of Salmonella Spp To the Bactericidal Activimentioning

confidence: 99%

Searching for Outer Membrane Proteins Typical of Serum-Sensitive and Serum-Resistant Phenotypes of Salmonella

Futoma-Kołoch¹,

Bugla-Płoskońska²,

Sarowska³

2012

Salmonella - Distribution, Adaptation, Control Measures and Molecular Technologies

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“…The isolation of OMPs was performed according to Murphy and Bartos [ 37 ] with minor modifications [ 20 , 38 ]. Bacterial strains were cultured overnight at 37 °C in 25 mL LB medium (Merck, Kenilworth, NJ, USA).…”

Section: Methodsmentioning

confidence: 99%

Relationship of Triamine-Biocide Tolerance of Salmonella enterica Serovar Senftenberg to Antimicrobial Susceptibility, Serum Resistance and Outer Membrane Proteins

Futoma-Kołoch

Dudek

Kapczyńska

et al. 2017

IJMS

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Abstract:A new emerging phenomenon is the association between the incorrect use of biocides in the process of disinfection in farms and the emergence of cross-resistance in Salmonella populations. Adaptation of the microorganisms to the sub-inhibitory concentrations of the disinfectants is not clear, but may result in an increase of sensitivity or resistance to antibiotics, depending on the biocide used and the challenged Salmonella serovar. Exposure of five Salmonella enterica subsp. enterica serovar Senftenberg (S. Senftenberg) strains to triamine-containing disinfectant did not result in variants with resistance to antibiotics, but has changed their susceptibility to normal human serum (NHS). Three biocide variants developed reduced sensitivity to NHS in comparison to the sensitive parental strains, while two isolates lost their resistance to serum. For S. Senftenberg, which exhibited the highest triamine tolerance (6 × MIC) and intrinsic sensitivity to 22.5% and 45% NHS, a downregulation of flagellin and enolase has been demonstrated, which might suggest a lower adhesion and virulence of the bacteria. This is the first report demonstrating the influence of biocide tolerance on NHS resistance. In conclusion, there was a potential in S. Senftenberg to adjust to the conditions, where the biocide containing triamine was present. However, the adaptation did not result in the increase of antibiotic resistance, but manifested in changes within outer membrane proteins' patterns. The strategy of bacterial membrane proteins' analysis provides an opportunity to adjust the ways of infection treatments, especially when it is connected to the life-threating bacteremia caused by Salmonella species.

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Reptiles as a Source of Salmonella O48—Clinically Important Bacteria for Children: The Relationship Between Resistance to Normal Cord Serum and Outer Membrane Protein Patterns

Cited by 11 publications

References 44 publications

Proteomic Analysis of Outer Membrane Proteins from Salmonella Enteritidis Strains with Different Sensitivity to Human Serum

Proteomic Analysis of Outer Membrane Proteins from Salmonella Enteritidis Strains with Different Sensitivity to Human Serum

Searching for Outer Membrane Proteins Typical of Serum-Sensitive and Serum-Resistant Phenotypes of Salmonella

Relationship of Triamine-Biocide Tolerance of Salmonella enterica Serovar Senftenberg to Antimicrobial Susceptibility, Serum Resistance and Outer Membrane Proteins

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