Reprogramming of Trophoblast Stem Cells into Pluripotent Stem Cells by Oct4

Wu, Tong; Wang, Haitao; He, Jing; Kang, Lan; Jiang, Yonghua; Liu, Jinchao; Zhang, Yu; Kou, Zhaohui; Liu, Lijun; Zhang, Xuehong; Gao, Shaorong

doi:10.1002/stem.617

Cited by 64 publications

(58 citation statements)

References 49 publications

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“…Similarly, OE of Cdx2 or the additional TE-restricted TF Gata3 or Tcfap2c promotes transition of ES cells into trophoblast stem (TS)-like cells, which are similar to an in vitro counterpart of TE derived from preimplantation embryos (Kuckenberg et al 2010;Ralston et al 2010). In contrast, OE of Oct4 in TS cells promotes an ES celllike fate (Wu et al 2011). Several factors preferentially expressed in the TE (e.g., Cdx2, Gata3, and Tcfap2c) are involved in self-renewal of TS cells (Chawengsaksophak et al 1997;Auman et al 2002;Ralston et al 2010).…”

Section: Cdx2mentioning

confidence: 99%

Arid3a is essential to execution of the first cell fate decision via direct embryonic and extraembryonic transcriptional regulation

et al. 2014

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Despite their origin from the inner cell mass, embryonic stem (ES) cells undergo differentiation to the trophectoderm (TE) lineage by repression of the ES cell master regulator Oct4 or activation of the TE master regulator Caudal-type homeobox 2 (Cdx2). In contrast to the in-depth studies of ES cell self-renewal and pluripotency, few TE-specific regulators have been identified, thereby limiting our understanding of mechanisms underlying the first cell fate decision. Here we show that up-regulation and nuclear entry of AT-rich interactive domain 3a (Arid3a) drives TE-like transcriptional programs in ES cells, maintains trophoblast stem (TS) cell selfrenewal, and promotes further trophoblastic differentiation both upstream and independent of Cdx2. Accordingly, Arid3a-/-mouse post-implantation placental development is severely impaired, resulting in early embryonic death. We provide evidence that Arid3a directly activates TE-specific and trophoblast lineage-specific genes while directly repressing pluripotency genes via differential regulation of epigenetic acetylation or deacetylation. Our results identify Arid3a as a critical regulator of TE and placental development through execution of the commitment and differentiation phases of the first cell fate decision.

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Section: Cdx2mentioning

confidence: 99%

Arid3a is essential to execution of the first cell fate decision via direct embryonic and extraembryonic transcriptional regulation

et al. 2014

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“…Pou5f1 represses Cdx2 transcription in ES cells most probably through Pou5f1 binding sites located in the upstream promoter and intron 1 region of Cdx2 (Niwa et al 2005, Loh et al 2006. Furthermore, overexpression of Pou5f1 in newly derived TS cells (ten passages) can drive these into the pluripotent ES (ICM-like) state (Wu et al 2011). Even well-established TS cells can be reverted to ES cells, though requiring a cocktail of pluripotency genes including Pou5f1 (Kuckenberg et al 2011).…”

Section: Genes Involved In Trophoblast Commitmentmentioning

confidence: 99%

Trophoblast development

Pfeffer¹,

Pearton²

2012

Reproduction

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This review summarises current knowledge about the specification, commitment and maintenance of the trophoblast lineage in mice and cattle. Results from gene expression studies, in vivo loss-of-function models and in vitro systems using trophoblast and embryonic stem cells have been assimilated into a model seeking to explain trophoblast ontogeny via gene regulatory networks. While trophoblast differentiation is quite distinct between cattle and mice, as would be expected from their different modes of implantation, recent studies have demonstrated that differences arise much earlier during trophoblast development.

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“…Importantly, overexpression of Cdx2 in ESCs leads to TSC-like cells with highly similar morphology, developmental potential, and gene expression as embryoderived TSCs [16,22,23]. Similarly, TSCs can be converted to ESC-like iPSC by overexpressing Oct4 [24,25]. Likewise, ESCs can be converted to XEN cells using growth factors or PE transcription factors [12,[26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%