Reprogramming of H3K9bhb at Regulatory Elements is an Epigenetic Feature of Fasting in the Small Intestine

Terranova, Christopher; Stemler, Kristina M.; Barrodia, Praveen; Jeter-Jones, Sabrina; Ge, Zhongqi; Bonilla, Marimar de la Cruz; Raman, Ayush T.; Cheng, Chia-Wei; Allton, Kendra L.; Yılmaz, Ömer H.; Barton, Michele C.; Rai, Kunal; Piwnica‐Worms, Helen

doi:10.2139/ssrn.3631381

Cited by 2 publications

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“…It is also reported that H3K9 kbhb enhances VEGF gene expression, attenuating endothelium injury in diabetic rats. Terranova et al (15) demonstrated that H3K9 kbhb is related to active chromatin states and plays a vital role in the expression of metabolic genes (16). These findings illustrated that kbhb plays a vital role in maintaining cellular physiology.…”

Section: Introductionmentioning

confidence: 97%

Proteome-wide analysis of lysine β-hydroxybutyrylation in the myocardium of diabetic rat model with cardiomyopathy

Luo

He²,

Luo

et al. 2023

Front. Cardiovasc. Med.

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Lysine ß-hydroxybutyrylation (kbhb), a novel modification of lysine residues with the ß-hydroxybuty group, is associated with ketone metabolism in numerous species. However, its potential role in diabetes, especially in diabetic cardiomyopathy (DCM), remains largely unexplored. In this study, using affinity enrichment and liquid chromatography-mass spectrometry (LC-MS/MS) method, we quantitatively analyze the kbhb residues on heart tissues of a DCM model rat. A total of 3,520 kbhb sites in 1,089 proteins were identified in this study. Further analysis showed that 336 kbhb sites in 143 proteins were differentially expressed between the heart tissues of DCM and wild-type rats. Among them, 284 kbhb sites in 96 proteins were upregulated, while 52 kbhb sites in 47 proteins were downregulated. Bioinformatic analysis of the proteomic results revealed that these kbhb-modified proteins were widely distributed in various components and involved in a wide range of cellular functions and biological processes (BPs). Functional analysis showed that the kbhb-modified proteins were involved in the tricarboxylic acid cycle, oxidative phosphorylation, and propanoate metabolism. Our findings demonstrated how kbhb is related to many metabolic pathways and is mainly involved in energy metabolism. These results provide the first global investigation of the kbhb profile in DCM progression and can be an essential resource to explore DCM’s pathogenesis further.

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Section: Introductionmentioning

confidence: 97%

Proteome-wide analysis of lysine β-hydroxybutyrylation in the myocardium of diabetic rat model with cardiomyopathy

Luo

He²,

Luo

et al. 2023

Front. Cardiovasc. Med.

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“…In recent years, it has become increasingly clear that energy availability can profoundly impact stem cell function, with dietary regimens such as fasting and ketogenic diets exerting notable effects on stem cell biology ( Cerletti et al, 2012 ; Cheng et al, 2014 ; Tang et al, 2016 ; Forni et al, 2017 ; Mihaylova et al, 2018 ; Long et al, 2019 ; Mishina et al, 2021 ; Novak et al, 2021 ; Terranova et al, 2021 ; Benjamin et al, 2022 ). Interestingly, some of the stem cell adaptations to fasting are linked to the presence of ketone bodies ( Cheng et al, 2019 ; Benjamin et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Regulation of adult stem cell function by ketone bodies

Andersen,

Poulsen,

Farup

et al. 2023

Front. Cell Dev. Biol.

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Adult stem cells play key roles in tissue homeostasis and regeneration. Recent evidence suggests that dietary interventions can significantly impact adult stem cell function. Some of these effects depend on ketone bodies. Adult stem cells could therefore potentially be manipulated through dietary regimens or exogenous ketone body supplementation, a possibility with significant implications for regenerative medicine. In this review we discuss recent findings of the mechanisms by which ketone bodies could influence adult stem cells, including ketogenesis in adult stem cells, uptake and transport of circulating ketone bodies, receptor-mediated signaling, and changes to cellular metabolism. We also discuss the potential effects of ketone bodies on intracellular processes such as protein acetylation and post-transcriptional control of gene expression. The exploration of mechanisms underlying the effects of ketone bodies on stem cell function reveals potential therapeutic targets for tissue regeneration and age-related diseases and suggests future research directions in the field of ketone bodies and stem cells.

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Reprogramming of H3K9bhb at Regulatory Elements is an Epigenetic Feature of Fasting in the Small Intestine

Cited by 2 publications

References 0 publications

Proteome-wide analysis of lysine β-hydroxybutyrylation in the myocardium of diabetic rat model with cardiomyopathy

Proteome-wide analysis of lysine β-hydroxybutyrylation in the myocardium of diabetic rat model with cardiomyopathy

Regulation of adult stem cell function by ketone bodies

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