Reprogramming of glucose metabolism of cumulus cells and oocytes and its therapeutic significance

Imanaka, Shogo; Shinmoto, Hiroshi; Kobayashi, Hiroshi

doi:10.1007/s43032-021-00505-6

Cited by 20 publications

(26 citation statements)

References 134 publications

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“…They showed that the combination of alginate-gelatin is eligible to increase angiogenesis-related tyrosine kinases such as Tie-1, -2, VEGFR-1, and − 2 [11]. We also found that CCs efficiently expressed ER which correlates with the dynamic activity of these cells [21,22]. It has been shown that the culture of cells on gelatin cross-linked matrices supports appropriate cell growth and survival by providing several motifs [23].…”

Section: Discussionmentioning

confidence: 55%

Decellularized bovine ovarian niche restored the function of cumulus and endothelial cells

et al. 2022

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Objective: Recently, the decellularization technique is introduced as one of the tissue engineering procedures for the treatment of various deficiencies. Here, we aimed to assess the dynamic activity of CCs and HUVECs within decellularized bovine ovarian tissue transplanted subcutaneously in rats. Ovarian tissue was decellularized using a cocktail consisting of different chemicals, and the efficiency of decellularization was assessed using hematoxylin-eosin and DAPI staining. The cell survival was evaluated using an LDH leakage assay. Thereafter, decellularized samples were recellularized using HUVECs and CCs, encapsulated inside alginate (1.2%)-gelatin, (1%) hydrogel, and transplanted subcutaneously to rats. The existence of CD31- and estrogen-positive cells was assessed using immunohistochemistry staining. Results: Bright-field imaging and DAPI staining revealed the lack of nuclei with naive matrix structure in ovarian tissue subjected to decellularization protocol. SEM imaging revealed a normal matrix in decellularized ovaries. LDH assay showed a lack of cytotoxicity for CCs after 7-days compared to the control group. Immunohistochemistry staining showed both CD31- and estrogen-positive cells in CCs + HUVECs compared to the CCs group. CD31 cells appeared with flattened morphology aligned with matrix fibers. The existence of estrogen and CD31 positive cells showed the efficiency of decellularized ovarian tissue to restore cellular function and activity.

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Section: Discussionmentioning

confidence: 55%

Decellularized bovine ovarian niche restored the function of cumulus and endothelial cells

et al. 2022

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“…When the energy charge in cells is lower, pyruvate dehydrogenase is activated by inhibiting the kinase. [76][77][78] According to our metabolomic data, there was an increase of ATP/ADP ratio following exposure to either copper ions or the…”

Section: Glycolysismentioning

confidence: 82%

Metabolomic and bioenergetic responses of human hepatocellular carcinoma cells following exposure to commercial copper hydroxide nanopesticide

Li¹,

Qin²,

Kong³

et al. 2022

Environ. Sci.: Nano

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“…7 The bidirectional communication between oocytes and granulosa cells are mediated by energy metabolism via glycolysis and OXPHOS. 39 Oocytes preferentially utilize the glycolytic activity of granulosa cells to meet their energy requirements for growth, maturation, and ovulation through the uptake of glycolytic metabolites such as pyruvate and lactate. 39,40 Therefore, oocyte maturation, fertilization, and pre-implantation embryo development are linked with the mitochondria-dependent bioenergetic capacity.…”

Section: Altered Mitochondrial Functionmentioning

confidence: 99%

“…39 Oocytes preferentially utilize the glycolytic activity of granulosa cells to meet their energy requirements for growth, maturation, and ovulation through the uptake of glycolytic metabolites such as pyruvate and lactate. 39,40 Therefore, oocyte maturation, fertilization, and pre-implantation embryo development are linked with the mitochondria-dependent bioenergetic capacity. 41 The number of oocyte mitochondria increase to more than 100 000 through folliculogenesis to provide the energy necessary for the development of the embryo post fertilization.…”

Section: Altered Mitochondrial Functionmentioning

confidence: 99%

“…42 Mitochondria generate energy in the form of ATP through the electron transport chain (ETC) by inducing OXPHOS, but release ROS as by-products. 39,43 The biologically important ROS include superoxide anions, hydrogen peroxide or hydroxyl radicals. 39,43 Mitochondria control ROS production and monitor redox state through scavenger activity, antioxidant production, and alterations in fission and fusion balance.…”

Section: Altered Mitochondrial Functionmentioning

confidence: 99%

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Role of the mitophagy‐apoptosis axis in the pathogenesis of polycystic ovarian syndrome

Kobayashi,

Shigetomi,

Matsubara

et al. 2024

J of Obstet and Gynaecol

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AimPolycystic ovary syndrome (PCOS) is a common endocrine disorder characterized by menstrual irregularities, androgen excess, and polycystic ovarian morphology, but its pathogenesis remains largely unknown. This review focuses on how androgen excess influences the molecular basis of energy metabolism, mitochondrial function, and mitophagy in granulosa cells and oocytes, summarizes our current understanding of the pathogenesis of PCOS, and discuss perspectives on future research directions.MethodsA search of PubMed and Google Scholar databases were used to identify relevant studies for this narrative literature review.ResultsFemale offspring born of pregnant animals exposed to androgens recapitulates the PCOS phenotype. Abnormal mitochondrial morphology, altered expression of genes related to glycolysis, mitochondrial biogenesis, fission/fusion dynamics, and mitophagy have been identified in PCOS patients and androgenic animal models. Androgen excess causes uncoupling of the electron transport chain and depletion of the cellular adenosine 5′‐triphosphate pool, indicating further impairment of mitochondrial function. A shift toward mitochondrial fission restores mitochondrial quality control mechanisms. However, prolonged mitochondrial fission disrupts autophagy/mitophagy induction due to loss of compensatory reserve for mitochondrial biogenesis. Disruption of compensatory mechanisms that mediate the quality control switch from mitophagy to apoptosis may cause a disease phenotype. Furthermore, genetic predisposition, altered expression of genes related to glycolysis and oxidative phosphorylation, or a combination of these factors may also contribute to the development of PCOS.ConclusionIn conclusion, fetuses exposed to a hyperandrogenemic intrauterine environment may cause the PCOS phenotype possibly through disruption of the compensatory regulation of the mitophagy‐apoptosis axis.

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Reprogramming of glucose metabolism of cumulus cells and oocytes and its therapeutic significance

Cited by 20 publications

References 134 publications

Decellularized bovine ovarian niche restored the function of cumulus and endothelial cells

Decellularized bovine ovarian niche restored the function of cumulus and endothelial cells

Metabolomic and bioenergetic responses of human hepatocellular carcinoma cells following exposure to commercial copper hydroxide nanopesticide

Role of the mitophagy‐apoptosis axis in the pathogenesis of polycystic ovarian syndrome

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