Reprogramming of cells using modified mRNA

Pandit, Vaibhav; Nesbitt, Robert Sterling; Macione, James; Kotha, Shiva P.

doi:10.1109/nebc.2011.5778580

Cited by 2 publications

(2 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A reprogramming method using mRNA has been developed in recent years ( Figure 4 a) [ 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 ]. Similar to mRNA vaccines, mRNA reprogramming creates iPSCs by introducing mRNA containing genetic information to create cell reprogramming factors in cells and expressed target proteins.…”

Section: Mrna For Cell Reprogrammingmentioning

confidence: 99%

“…Reprogramming using microRNA is an effective method, but the only microRNAs that have been found to be involved in reprogramming are mir-302 [80], mir-372 [81], the miR-17-92 cluster [82], mir-19 [83], mir-524 [84], mir-371 [85], and mir-31 [86]; thus, in the future, it is necessary to explore the applicability of various microRNAs [87]. • High reprogramming efficiency A reprogramming method using mRNA has been developed in recent years (Figure 4a) [88][89][90][91][92][93][94][95][96][97][98][99][100][101][102][103][104][105][106][107]. Similar to mRNA vaccines, mRNA reprogramming creates iPSCs by introducing mRNA containing genetic information to create cell reprogramming factors in cells and expressed target proteins.…”

Section: Mrna For Cell Reprogrammingmentioning

confidence: 99%

See 1 more Smart Citation

Cell Reprogramming and Differentiation Utilizing Messenger RNA for Regenerative Medicine

Inagaki

2023

JDB

View full text Add to dashboard Cite

The COVID-19 pandemic generated interest in the medicinal applications of messenger RNA (mRNA). It is expected that mRNA will be applied, not only to vaccines, but also to regenerative medicine. The purity of mRNA is important for its medicinal applications. However, the current mRNA synthesis techniques exhibit problems, including the contamination of undesired 5′-uncapped mRNA and double-stranded RNA. Recently, our group developed a completely capped mRNA synthesis technology that contributes to the progress of mRNA research. The introduction of chemically modified nucleosides, such as N1-methylpseudouridine and 5-methylcytidine, has been reported by Karikó and Weissman, opening a path for the practical application of mRNA for vaccines and regenerative medicine. Yamanaka reported the production of induced pluripotent stem cells (iPSCs) by introducing four types of genes using a retrovirus vector. iPSCs are widely used for research on regenerative medicine and the preparation of disease models to screen new drug candidates. Among the Yamanaka factors, Klf4 and c-Myc are oncogenes, and there is a risk of tumor development if these are integrated into genomic DNA. Therefore, regenerative medicine using mRNA, which poses no risk of genome insertion, has attracted attention. In this review, the author summarizes techniques for synthesizing mRNA and its application in regenerative medicine.

show abstract

Section: Mrna For Cell Reprogrammingmentioning

confidence: 99%

Section: Mrna For Cell Reprogrammingmentioning

confidence: 99%

Cell Reprogramming and Differentiation Utilizing Messenger RNA for Regenerative Medicine

Inagaki

2023

JDB

View full text Add to dashboard Cite

show abstract

Cell Reprogramming and Differentiation Utilizing Messenger RNA for Regenerative Medicine

Inagaki

2023

Preprint

View full text Add to dashboard Cite

The COVID-19 pandemic stimulated attention to the medicinal applications of messenger RNA (mRNA). mRNA is expected to be applied not only to vaccines but also to regenerative medicines. The purity of mRNA is important for its medicinal application. However, the current mRNA synthesis techniques have problems, e.g., contamination of undesired 5’-uncapped mRNA and double-stranded RNAs. Recently, our group developed a completely capped mRNA synthesis technology that contributed to the progress of mRNA research. The introduction of chemically modified nucleosides, e.g., N1-methylpseudouridine and 5-methylcytidine, have been demonstrated by Dr. Karikó and Dr. Weissman which opened the practical application of mRNA for vaccines and regenerative medicines. Dr. Yamanaka reported the production of Induced Pluripotent Stem Cells (iPSCs) by the introduction of 4 types of genes using a retrovirus vector. iPSCs are widely used for research about regenerative medicines and the preparation of disease models to screen new drug candidates. Of the Yamanaka factors, Klf4 and c-Myc are oncogenes, and there is a tumor development risk if these are integrated into genomic DNA. Therefore, regenerative medicines using mRNA, which has no risk of genome insertion, have attracted attention. In this review, we summarized the synthesis of mRNA and its application for regenerative medicine.

show abstract

Reprogramming of cells using modified mRNA

Cited by 2 publications

References 2 publications

Cell Reprogramming and Differentiation Utilizing Messenger RNA for Regenerative Medicine

Cell Reprogramming and Differentiation Utilizing Messenger RNA for Regenerative Medicine

Cell Reprogramming and Differentiation Utilizing Messenger RNA for Regenerative Medicine

Contact Info

Product

Resources

About