Reprogramming human adipose tissue stem cells using epidermal keratinocyte extracts

Xie, Feng; Tang, Xinjie; Qun, Zhang; Deng, Chaohua

doi:10.3892/mmr.2014.2711

Cited by 8 publications

(5 citation statements)

References 19 publications

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“…The hADSCs exhibited favorable characteristics, including adherence, proliferation, and morphological changes indicative of differentiation into keratinocyte-like cells. These findings align with previous studies that have underscored the adaptability of ADSCs for regenerative applications in skin tissue engineering. − The scaffold’s role in facilitating cell attachment, viability, and proliferation is pivotal for its success in tissue engineering. The high cell viability observed in the MTT assay indicates excellent biocompatibility and low cytotoxicity, essential for maintaining a conductive environment for cell growth and tissue regeneration.…”

Section: Discussionsupporting

confidence: 92%

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Hazrati,

Alizadeh,

Soltani

et al. 2024

ACS Omega

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Platelet-derived growth factor-BB (PDGF-BB) is a polypeptide growth factor generated by platelet granules faced to cytokines. It plays a role in forming and remodeling various tissue types, including epithelial tissue, through interaction with cell-surface receptors on most mesenchymal origin cells. However, it breaks down quickly in biological fluids, emphasizing the importance of preserving them from biodegradation. To address this challenge, we formulated and evaluated PDGF-encapsulated nanospheres (PD@PCEC) using polycaprolactone-polyethylene glycol-polycaprolactone. PD@PCECs were fabricated through the triple emulsion methodology and optimized by using the Box− Behnken design. The encapsulation efficiency (EE) of nanoencapsulated PDGF-BB was investigated concerning four variables: stirring rate (X1), stirring duration (X2), poly(vinyl alcohol) concentration (X3), and PDGF-BB concentration (X4). The selected optimized nanospheres were integrated into a gelatin-collagen scaffold (PD@PCEC@GC) and assessed for morphology, biocompatibility, in vitro release, and differentiation-inducing activity in human adipose-derived stem cells (hADSCs). The optimized PD@PCEC nanospheres exhibited a particle size of 177.9 ± 91 nm, a zeta potential of 5.2 mV, and an EE of 87.7 ± 0.44%. The release profile demonstrated approximately 85% of loaded PDGF-BB released during the first 360 h, with a sustained release over the entire 504 h period, maintaining bioactivity of 87.3%. The study also included an evaluation of the physicochemical properties of the scaffolds and an assessment of hADSC adhesion to the scaffold's surface. Additionally, hADSCs cultivated within the scaffold effectively differentiated into keratinocyte-like cells (KLCs) over 21 days, evidenced by morphological changes and upregulation of keratinocyte-specific genes, including cytokeratin 18, cytokeratin 19, and involucrin, at both transcriptional and protein levels.

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Section: Discussionsupporting

confidence: 92%

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Hazrati,

Alizadeh,

Soltani

et al. 2024

ACS Omega

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“…The goal of regenerative medicine is to replace diseased tissues with functional cells or cellular products. Many studies have sought to generate KCs from fibroblasts [ 29 , 52 ], adipose tissue stem cells [ 53 ], and mesenchymal cells via reprogramming [ 54 ]. Due to its critical role in epidermal development [ 55 ], TP63 was once combined with other TFs and used for this purpose [ 29 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Functional Evidence Show Similarities between Human Amniotic Epithelial Stem Cells and Keratinocytes

Liu

Zheng

et al. 2021

Cells

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Amniotic epithelial stem cells (AESCs) are considered as potential alternatives to keratinocytes (KCs) in tissue-engineered skin substitutes used for treating skin damage. However, their clinical application is limited since similarities and distinctions between AESCs and KCs remain unclear. Herein, a transcriptomics analysis and functional evaluation were used to understand the commonalities and differences between AESCs and KCs. RNA-sequencing revealed that AESCs are involved in multiple epidermis-associated biological processes shared by KCs and show more similarity to early stage immature KCs than to adult KCs. However, AESCs were observed to be heterogeneous, and some possessed hybrid mesenchymal and epithelial features distinct from KCs. A functional evaluation revealed that AESCs can phagocytose melanosomes transported by melanocytes in both 2D and 3D co-culture systems similar to KCs, which may help reconstitute pigmented skin. The overexpression of TP63 and activation of NOTCH signaling could promote AESC stemness and improve their differentiation features, respectively, bridging the gap between AESCs and KCs. These changes induced the convergence of AESC cell fate with KCs. In future, modified reprogramming strategies, such as the use of small molecules, may facilitate the further modulation human AESCs for use in skin regeneration.

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“…In a previous study, the same induction medium formulation was used for mouse ASC differentiation to a keratinocyte cell lineage (Shokrgozar et al, 2012). Other studies reported hASC differentiation to epithelial-like cells, but only following their exposure to retinoic acid supplemented culture medium (Chan et al, 2012;Yan et al, 2013), cocultivation with human foreskin keratinocytes in a Transwell culture system (Chavez-Munoz et al, 2013), or by reprogramming hASCs using nuclear and cytoplasmic extracts from epidermal keratinocytes (Xie et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Human adipose-derived stem cells differentiation into epidermal cells and interaction with human keratinocytes in coculture

Gaspar¹,

Constantin²,

Seciu³

et al. 2016

Turk J Biol

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Introduction Current standard therapy for full-thickness injuries, like chronic wounds or burns, is transplantation with autologous skin grafts. In the last decade, epithelial sheets cultivated with keratinocytes and dermal fibroblasts were typically used for engraftment of patients with extensive skin injuries (Atiyeh and Costagliola, 2007). Although they appeared very promising, several limitations occurred over time, such as long culturing periods, low rates of cellular viability, risk of infection, and high cost (Lootens et al., 2013). Moreover, cell division arrest was reported after 30 to 40 population doublings, thus limiting the life span of keratinocytes in culture (Chapman et al., 2010). Stem cells represent a viable therapeutic alternative for skin repair due to their specific properties of self-renewal, high proliferation rate, and ability to develop terminally differentiated cells (Markeson et al., 2013). They can be easily isolated, in vitro multiplied, and used in autologous methodologies of regenerative medicine without the risk of tumor development (Silva et al., 2013). Many studies have reported isolation of mesenchymal stem cells (MSCs) from bone marrow (Bara et al., 2014), adipose tissue (Brzoska et al., 2005), or Wharton's jelly (Crisan, 2013), and their differentiation in cells of mesodermal origin like osteocytes, adipocytes, chondrocytes, and myocytes. Little research was focused on MSC differentiation into ectodermal lineage, like epidermal cells, in order to develop stem cell-based skin therapies (Zuk, 2013). To our knowledge, the mechanisms of adipose tissue-derived stem cells (ASCs) transdifferentiation into skin cell lineages and their therapeutic effects are currently discussed (Derby et al., 2014). The present study aimed to investigate the capacity of human ASCs to differentiate in vitro into a keratinocyte lineage, using specific induction medium containing a mixture of growth factors. The expression of distinctive keratinocyte markers was analyzed by immunofluorescence and RT-PCR. Additionally, the interaction between ASCs and HaCaT keratinocytes was investigated in two coculture assays using a viability test, light microscopy, and flow cytometry techniques.

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Reprogramming human adipose tissue stem cells using epidermal keratinocyte extracts

Cited by 8 publications

References 19 publications

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Transcriptomic and Functional Evidence Show Similarities between Human Amniotic Epithelial Stem Cells and Keratinocytes

Human adipose-derived stem cells differentiation into epidermal cells and interaction with human keratinocytes in coculture

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