Reprogramming cellular metabolism to increase the efficiency of microbial cell factories

Ding, Qiang; Li, Liming

doi:10.1080/07388551.2023.2208286

Cited by 3 publications

(2 citation statements)

References 136 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Calvin-Benson-Bassham (CBB) cycle is a widely known natural CO 2 assimilation pathway among eukaryotes and prokaryotes with ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO, rbcLS ) and phosphoribulokinase ( prk ) as the key enzymes. , This pathway has an interconnection with the pentose phosphate (PP) pathway as a bypass shunt from ribose 5-phosphate (Ru5P) to pentose to 3-phosphoglycerate (3PG). The PP pathway of E. coli is different from other CO 2 -fixing organisms which redirects the flux from the decomposed hexose to glyceraldehyde-3-phosphate (GAP) and fructose-6-phosphate (F6P). , Previously, the non-native CO 2 biomitigation system via the CBB cycle was established in E. coli K-12 and BL21 for recycling the CO 2 emission during chemical biosynthesis. − Ron Milo’s group effectively harnessed E. coli to synthesize biomass from CO 2 using a similar artificial design, which was integrated with a reducing power system from C1 organic compounds.…”

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Utilizing Ribose 1,5-Bisphosphate Isomerase and RuBisCO-Equipped Escherichia coli Nissle for Low-Carbon Footprint GABA Production

Effendi,

Toya,

Shimizu

et al. 2024

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

Escherichia coli Nissle 1917 (EcN), the only probiotic E. coli, has been exploited as a promising chemical bioproducer due to possessing unique mutations under acidic conditions. To bolster its sustainability, a novel CO 2 -recycling system was reconstructed by coexpressing ribose-1,5-bisphosphate isomerase (R15Pi) and ribulose-1,5-bisphosphate carboxylaseoxygenase (RuBisCO) (i.e., RR plasmid). The function of RR was examined through the transcription level of the R15Pgenerating gene (phnN), showing higher mRNA in EcN. Afterward, the RR-equipped EcN strain was utilized for recycling CO 2 release during γ-aminobutyric acid (GABA) synthesis, improving the yield by 12, 12.5, and 14% through CO 2 assimilation in glucose, acetate, and glycerol medium, respectively. RR with a low copy GadB plasmid (i.e., RR+LG strain) successfully assimilated CO 2 of 27−37% within the three mediums. The artificial CO 2fixing system was successfully reconstructed in EcN via R15Pi and RuBisCO, thus manifesting the prospects of EcN as a low-carbonfeaturing microbial cell factory by a new pathway.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Utilizing Ribose 1,5-Bisphosphate Isomerase and RuBisCO-Equipped Escherichia coli Nissle for Low-Carbon Footprint GABA Production

Effendi,

Toya,

Shimizu

et al. 2024

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

show abstract

Strategies to increase the robustness of microbial cell factories

Xu,

Lin,

Zhang

et al. 2024

Adv. Biotechnol.

View full text Add to dashboard Cite

Engineering microbial cell factories have achieved much progress in producing fuels, natural products and bulk chemicals. However, in industrial fermentation, microbial cells often face various predictable and stochastic disturbances resulting from intermediate metabolites or end product toxicity, metabolic burden and harsh environment. These perturbances can potentially decrease productivity and titer. Therefore, strain robustness is essential to ensure reliable and sustainable production efficiency. In this review, the current strategies to improve host robustness were summarized, including knowledge-based engineering approaches, such as transcription factors, membrane/transporters and stress proteins, and the traditional adaptive laboratory evolution based on natural selection. Computation-assisted (e.g. GEMs, deep learning and machine learning) design of robust industrial hosts was also introduced. Furthermore, the challenges and future perspectives on engineering microbial host robustness are proposed to promote the development of green, efficient and sustainable biomanufacturers.

show abstract

Chemical components and health benefits of Rubus suavissimus S. Lee (Chinese sweet tea) and the production method of rubusoside

Liu,

Wang,

Zhao

et al. 2024

Trends in Food Science & Technology

View full text Add to dashboard Cite

Reprogramming cellular metabolism to increase the efficiency of microbial cell factories

Cited by 3 publications

References 136 publications

Utilizing Ribose 1,5-Bisphosphate Isomerase and RuBisCO-Equipped Escherichia coli Nissle for Low-Carbon Footprint GABA Production

Utilizing Ribose 1,5-Bisphosphate Isomerase and RuBisCO-Equipped Escherichia coli Nissle for Low-Carbon Footprint GABA Production

Strategies to increase the robustness of microbial cell factories

Chemical components and health benefits of Rubus suavissimus S. Lee (Chinese sweet tea) and the production method of rubusoside

Contact Info

Product

Resources

About