Reproductive toxicologic effects of gossypol on male rabbits: biochemical, enzymatic, and electrolytic properties of seminal plasma

Shaaban, W. F.; Taha, Taha A.; El-Nouty, F. D.; El-Mahdy, Ahmed R.; Salem, M. H.

doi:10.1016/j.fertnstert.2007.06.077

Cited by 8 publications

(8 citation statements)

References 48 publications

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“…Thus, the present findings showing a gossypolinduced increase in the SFA/USFA ratio and a decrease in the percentage of oleic acid (Table 2) suggest a state of mineralocorticoid excess, which is in accordance with our Taha, Shaaban, EL-Nouty and Salem previous observations on rabbits (Shaaban et al, 2008). However, the percentage of mono-USFA palmitoleic was increased (Table 2) probably due to the effect of mineralocorticoid excess on the inhibition of delta-6 desaturase activity (Marra and de Alaniz, 1990).…”

Section: Fatty Acidssupporting

confidence: 93%

“…Vera et al (1987) reported that maximal incorporation of leucine into sperm outer dense fiber polypeptides occurred during spermatogenesis. This finding could support and explain the present results in which gossypol treatment resulted in a reduction in seminal plasma leucine concentration, which was associated with the disappearance of some of the outer dense fibers as depicted by electron microscopy (Shaaban, 2003). In addition, bovine spermatozoa were found to have functional mechanism for the active transport of glycine, which is enhanced at low hexose concentration (Dietz and Flipse, 1966).…”

Section: Amino Acidssupporting

confidence: 89%

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Molecular approach of gossypol-induced reproductive toxicity in male rabbits. Changes in seminal plasma amino acids and fatty acids

Taha

Shaaban

El-Nouty

et al. 2008

Animal

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This study was done to evaluate the effects of two sublethal doses of gossypol (4 and 20 mg/kg of BW, every other day) on some amino and fatty acid concentrations in male rabbit seminal plasma. Rabbits were chosen as an experimental animal owing to the fact that they are excellent model for reproductive toxicological effects. The experiment lasted 16 weeks and included two periods: a treatment period (first 8 weeks) where the animals were given the tested product, and a recovery period (second 8 weeks) where all drugs were withdrawn. Results showed that total amino acids (TAA), total essential amino acids (EAA), total non-essential amino acids (non-EAA) and EAA/non-EAA ratio were decreased in a dose-dependent manner during gossypol treatment. The deleterious effect on TAA concentrations was mainly due to the reduction in total EAA. However, these concentrations regained their normal values after gossypol cessation. Basic, acidic, neutral amino acids and basic/acidic amino acids ratio decreased in a dose-dependent manner by gossypol treatment. Additionally, gossypol administration caused decreases in total unsaturated fatty acids (USFA) and increases in total saturated fatty acids (SFA) and the SFA/USFA ratio in a dose-dependent manner. During the recovery period, total SFA and USFA showed significant reduction and significant increase, respectively, after gossypol withdrawal. In conclusion, gossypol administration affected rabbit seminal plasma concentrations of amino and fatty acids in a dose-dependant manner. Gossypol reduced TAA, total EAA and total non-EAA. Additionally, gossypol caused decreases in total USFA and increases in total SFA. These deleterious effects were associated with poor-quality semen observed in our previous studies.

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Section: Fatty Acidssupporting

confidence: 93%

Section: Amino Acidssupporting

confidence: 89%

Molecular approach of gossypol-induced reproductive toxicity in male rabbits. Changes in seminal plasma amino acids and fatty acids

Taha

Shaaban

El-Nouty

et al. 2008

Animal

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“…Other comparative studies on biochemical markers in seminal plasma and serum were focused mostly on a single marker, such as the level of Mg in seminal plasma and serum samples obtained from normal fertile men and patients with premature ejaculation [ 9 ], and the levels of Ca and Mg in seminal plasma and serum from 113 men [ 10 ]. Moreover, the levels of protein, electrolytes, enzymes, and other components in seminal plasma samples in animals such as brown bears [ 11 ], rabbits [ 12 ], stallion [ 13 ], and bactrian camels [ 14 ] have been investigated, but the useful information was very limited in these studies.…”

Section: Discussionmentioning

confidence: 99%

A Pilot Comparative Study of 26 Biochemical Markers in Seminal Plasma and Serum in Infertile Men

Feng

Lü

Zhang

et al. 2015

BioMed Research International

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Introduction. The relationships of the biochemical components in seminal plasma and serum, and their origins and physiological effects in male reproductive system have been poorly understood. Methods. Based on the calibration and quality control measures, 26 biochemical markers, in seminal plasma and serum samples from 36 male infertility patients with nonazoospermia were detected and compared. Results. Only PA was undetectable in all seminal plasma samples. There were significant differences of all other 24 biochemical markers in seminal plasma and serum (P < 0.05) except for UA (P = 0.214). There were rich proteins in seminal plasma, and globulin accounted for about 90%. There were also abundant enzymes in seminal plasma, and the activities of ALT, AST, AKP, GGT, LDH, CK, and αHBDH in seminal plasma were significantly higher than those in serum while ADA was inversely lower. There were relatively low levels of Glu, TG, TC, and hsCRP in seminal plasma, but Glu was undetectable in 8 of 36 cases. Conclusions. The differences of the levels of biochemical markers in seminal plasma and serum might be associated with the selective secretion of testis, epididymis and male accessory glands, and the specific environment needed for sperm metabolism and function maintenance.

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“…The exposure resulted in an increase in seminal plasma total protein, together with a decrease in total lipids and increased activities in seminal lactate dehydrogenase and alanine aminotransferase at both doses. These effects were reversible after withdrawal of gossypol in contrast with the increase in albumin, decrease in seminal plasma lactate dehydrogenase and aspartate aminotransferase activity and potassium levels which were irreversible (Shaaban et al, 2008).…”

Section: Data From Experimental Animalsmentioning

confidence: 84%

“…of gossypol for eight weeks, biochemical parameters such as electrolytes, protein, albumin, cholesterol and enzymes alanin aminotransferase (ALAT), lactate dehydrogenase (LDH) in seminal plasma were influenced by both doses of gossypol. Some of the changes were reversible (Shaaban et al, 2008).…”

Section: Rabbitsmentioning

confidence: 99%

Gossypol as undesirable substance in animal feed - Scientific Opinion of the Panel on Contaminants in the Food Chain

2009

EFSA Journal

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Reproductive toxicologic effects of gossypol on male rabbits: biochemical, enzymatic, and electrolytic properties of seminal plasma

Cited by 8 publications

References 48 publications

Molecular approach of gossypol-induced reproductive toxicity in male rabbits. Changes in seminal plasma amino acids and fatty acids

Molecular approach of gossypol-induced reproductive toxicity in male rabbits. Changes in seminal plasma amino acids and fatty acids

A Pilot Comparative Study of 26 Biochemical Markers in Seminal Plasma and Serum in Infertile Men

Gossypol as undesirable substance in animal feed - Scientific Opinion of the Panel on Contaminants in the Food Chain

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