Reproductive Toxicity of Zishen Yutai Pill in Rats: The Fertility and Early Embryonic Development Study (Segment I)

Abstract

Purpose. This study was aimed to investigate the reproductive toxicity of Zishen Yutai Pill (ZYP) on fertility and early embryonic development in rats. Methods. SD rats were randomly divided into 5 groups: vehicle control group (distilled water, i.g.), positive control group (80 mg/kg of cyclophosphamide, i.p.), and three ZYP-treated groups (3, 6, and 12 g/kg/d, i.e., 12x, 24x, and 48x clinical doses, i.g.). The high dose was set as the maximum gavage dosage. Results. Cyclophosphamide showed diverse hazards, such as decreased weight of male reproductive organs and sperm density (P < 0.05). However, there were no obvious effects of ZYP on physical signs, animal behavior, and survival rate, as well as on weight and food intake during the premating and gestation periods. Importantly, there were no significant adverse effects of ZYP on indexes of copulation, fecundity and fertility indexes, weights and coefficients of male reproductive organs, epididymal sperm number and motility, estrous cycle, preimplantation loss rate, and implantation rate. Besides, the numbers of live and resorbed fetuses per litter were not significantly altered. Conclusions. ZYP had no reproductive toxicities on fertility and early embryonic development in rats at 48x equivalent clinical doses.

Fertility and early embryonic development toxicity and toxicokinetic study of KFP‐H008 in Sprague–Dawley rats

“…It is suggested that attention be paid to the effect of drugs on the prostate in clinical study. Semen motility, vitality, and morphological abnormality were essential indicators of F I G U R E 6 The concentration of KFP-H008 in tissues and organs in male (a) and female (b) rats in toxicokinetic study epididymal maturation and testicular spermatogenesis for male fertility (Guo et al, 2018;Zhou et al, 2016).…”

“…It is suggested that attention be paid to the effect of drugs on the prostate in clinical study. Semen motility, vitality, and morphological abnormality were essential indicators of epididymal maturation and testicular spermatogenesis for male fertility (Guo et al, 2018; Zhou et al, 2016). Our findings showed no toxic effects of sperm motility and sperm morphology of male rats in each KFP‐H008 groups.…”

Network analysis and experimental approach to investigate the potential therapeutic mechanism of zishen yutai pills on premature ovarian insufficiency

“…In addition, ZSYTP facilitates the recovery from POI either when used singularly or in combination with other medicines [ [11] , [12] , [13] ]. The safety of ZSYTP is likewise reliable, showing no significant toxicity or adverse side effects [ [14] , [15] , [16] , [17] ]. Therefore, this drug may be a promising alternative to HRT.…”