Reproductive toxicity of enrofloxacin in Caenorhabditis elegans involves oxidative stress-induced cell apoptosis

Huang, Jingxiang; Liao, Lizi; Wang, Guowei; Du, Zhongkun; Wu, Zheng-Xing

doi:10.1016/j.jes.2022.07.002

Cited by 5 publications

(3 citation statements)

References 63 publications

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“…Previous studies have supported the idea that multiple antibiotics can generate harmful reactive oxygen species (ROS) regardless of drug-target interactions, and widely induce oxidative stress [12]. Antibiotic-induced oxidative stress as a toxic effect has been reported in animals and plants, which would interfere with their survival and development, leading to inhibition in growth and reproduction, physiological, biochemical and tissue alterations [13][14][15][16]. In addition, the impact of antibiotics on the microbiosphere is of great concern [17].…”

Section: Introductionmentioning

confidence: 95%

Cefotaxime Exposure-Caused Oxidative Stress, Intestinal Damage and Gut Microbial Disruption in Artemia sinica

Pang,

Zheng,

Wang

et al. 2024

Microorganisms

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Cefotaxime (CTX) is an easily detectable antibiotic pollutant in the water environment, but little is known about its toxic effects on aquatic invertebrates, especially on the intestine. Here, we determined the oxidative stress conditions of A. sinica under CTX exposure with five concentrations (0, 0.001, 0.01, 0.1 and 1 mg/L) for 14 days. After that, we focused on changes in intestinal tissue morphology and gut microbiota in A. sinica caused by CTX exposure at 0.01 mg/L. We found malondialdehyde (MDA) was elevated in CTX treatment groups, suggesting the obvious antibiotic-induced oxidative stress. We also found CTX exposure at 0.01 mg/L decreased the villus height and muscularis thickness in gut tissue. The 16S rRNA gene analysis indicated that CTX exposure reshaped the gut microbiota diversity and community composition. Proteobacteria, Actinobacteriota and Bacteroidota were the most widely represented phyla in A. sinica gut. The exposure to CTX led to the absence of Verrucomicrobia in dominant phyla and an increase in Bacteroidota abundance. At the genus level, eleven genera with an abundance greater than 0.1% exhibited statistically significant differences among groups. Furthermore, changes in gut microbiota composition were accompanied by modifications in gut microbiota functions, with an up-regulation in amino acid and drug metabolism functions and a down-regulation in xenobiotic biodegradation and lipid metabolism-related functions under CTX exposure. Overall, our study enhances our understanding of the intestinal damage and microbiota disorder caused by the cefotaxime pollutant in aquatic invertebrates, which would provide guidance for healthy aquaculture.

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Section: Introductionmentioning

confidence: 95%

Cefotaxime Exposure-Caused Oxidative Stress, Intestinal Damage and Gut Microbial Disruption in Artemia sinica

Pang,

Zheng,

Wang

et al. 2024

Microorganisms

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“…Their findings revealed that oxidative stress networks were the main targets for antimicrobial potentiation [ 110 ]. Huang et al (2023) suggested that enrofloxacin-induced reproductive toxicity was related to germ cell apoptosis under oxidative stress in Caenorhabditis elegans [ 111 ]. This study suggests that chronic exposure to FQs at certain levels in the environment induces reproductive toxicity in nematodes and might reduce soil sustainability.…”

Section: Environmental Toxicology Mechanisms Of Fqsmentioning

confidence: 99%

“…Oxidative stress [111] Increase in the activity of the antioxidant enzyme catalase and decrease in lipid peroxidation levels…”

Section: Reproductive Toxicity and Cell Apoptosismentioning

confidence: 99%

The Research Status, Potential Hazards and Toxicological Mechanisms of Fluoroquinolone Antibiotics in the Environment

Liu

Pan

et al. 2023

Antibiotics

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Fluoroquinolone antibiotics are widely used in human and veterinary medicine and are ubiquitous in the environment worldwide. This paper recapitulates the occurrence, fate, and ecotoxicity of fluoroquinolone antibiotics in various environmental media. The toxicity effect is reviewed based on in vitro and in vivo experiments referring to many organisms, such as microorganisms, cells, higher plants, and land and aquatic animals. Furthermore, a comparison of the various toxicology mechanisms of fluoroquinolone antibiotic residues on environmental organisms is made. This study identifies gaps in the investigation of the toxic effects of fluoroquinolone antibiotics and mixtures of multiple fluoroquinolone antibiotics on target and nontarget organisms. The study of the process of natural transformation toward drug-resistant bacteria is also recognized as a knowledge gap. This review also details the combined toxicity effect of fluoroquinolone antibiotics and other chemicals on organisms and the adsorption capacity in various environmental matrices, and the scarcity of data on the ecological toxicology evaluation system of fluoroquinolone antibiotics is identified. The present study entails a critical review of the literature providing guidelines for the government to control the discharge of pollutants into the environment and formulate policy coordination. Future study work should focus on developing a standardized research methodology for fluoroquinolone antibiotics to guide enterprises in the design and production of drugs with high environmental biocompatibility.

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Induction of the mitochondrial pathway of apoptosis by enrofloxacin in the context of the safety issue of its use in poultry

Grabowski,

Choszcz,

Wiśniewska

et al. 2024

Apoptosis

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Reproductive toxicity of enrofloxacin in Caenorhabditis elegans involves oxidative stress-induced cell apoptosis

Cited by 5 publications

References 63 publications

Cefotaxime Exposure-Caused Oxidative Stress, Intestinal Damage and Gut Microbial Disruption in Artemia sinica

Cefotaxime Exposure-Caused Oxidative Stress, Intestinal Damage and Gut Microbial Disruption in Artemia sinica

The Research Status, Potential Hazards and Toxicological Mechanisms of Fluoroquinolone Antibiotics in the Environment

Induction of the mitochondrial pathway of apoptosis by enrofloxacin in the context of the safety issue of its use in poultry

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