Reproductive toxicity of azoxystrobin to adult zebrafish (Danio rerio)

Cao, Fangjie; Zhu, Li; Li, Hui; Song, Yu; Wang, Chengju; Qiu, Lihong

doi:10.1016/j.envpol.2016.09.015

Cited by 102 publications

(55 citation statements)

References 43 publications

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“…Similar to our result, Xu et al () also found that upregulation in StAR, 3β‐HSD and 17β‐HSD transcripts in the ovary of female zebrafish treated with Tris (2‐butoxyethyl) phosphate. Additionally, many reports are in concordance to our result that upregulation of 3β‐HSD and 17β‐HSD transcripts in the female gonad of zebrafish exposed to azoxystrobin (Cao et al, ) while StAR and 17β‐HSD in ibuprofen and mefenamic acid exposed gonad (Ji, Liu, et al, ). Thus, an increase in the transcript level of StAR, 3β‐HSD and 17β‐HSD in our study could reveal the biphasic effect of ZEA in HPG axis disruption.…”

Section: Discussionsupporting

confidence: 92%

“…However, a significant decrease in GSI was observed in higher-dose ZEA-exposed zebrafish. Similarly, Cao et al (2016) found no changes in HSI and a significant reduction in GSI of female zebrafish exposed to azoxystrobin. Moreover, Ji, Hong, Kho, and Choi (2013) also report a significant decrease in GSI had been reported in bisphenol S-exposed zebrafish.…”

Section: Discussionmentioning

confidence: 89%

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Mycotoxin zearalenone induced gonadal impairment and altered gene expression in the hypothalamic–pituitary–gonadal axis of adult female zebrafish (Danio rerio)

Muthulakshmi

Fallah

Habibi

et al. 2018

J of Applied Toxicology

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In the present study, we aimed to assess the adverse effects of zearalenone (ZEA) at environmentally relevant concentrations (0.5, 1, 5 and 10 μg l ) on hypothalamic-pituitary-gonadal axis associated reproductive function using zebrafish model. ZEA was exposed to female zebrafish for 21 days to assess growth indices such as condition factor, hepatosomatic index, gonadosomatic index and caspase 3 activity. Further, expression of estrogen receptor (ER) α and CYP19a1b genes in the brain, ERα and vitellogenin (Vtg) genes in the liver and follicle-stimulating hormone receptor, luteinizing hormone receptor, ERα, steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase (HSD), 17-βHSD and CYP19a1 genes in the ovary were also investigated. Our results showed that there were no significant changes in the condition factor and hepatosomatic index, whereas a significant (P < .05) reduction in the gonadosomatic index, increase in caspase 3 activities and Vtg expression was observed at higher concentration. However, no significant changes were observed at lower treatment levels. Further, we also observed significant (P < .05) upregulation in ERα, Vtg, luteinizing hormone receptor, steroidogenic acute regulatory protein, 3β-HSD, 17β-HSD, CYP19a1 and CYP19a1b genes in treatment groups with higher levels of ZEA. Moreover, in histopathological examination, we observed oocyte atresia and oocyte membrane detachment in ovaries at the highest concentration. In conclusion, the present study revealed the negative impact of ZEA on zebrafish reproductive system by involvement of the hypothalamic-pituitary-gonadal axis-associated reproductive function.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 89%

Mycotoxin zearalenone induced gonadal impairment and altered gene expression in the hypothalamic–pituitary–gonadal axis of adult female zebrafish (Danio rerio)

Muthulakshmi

Fallah

Habibi

et al. 2018

J of Applied Toxicology

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“…Gonadotropin hormones include follicle-stimulating hormone (FSH) and luteinizing hormone (LH), acting on the gonads to direct steroidogenesis as well as gametogenesis . Growing evidence demonstrates that endocrine disrupting chemicals (EDCs) can disrupt the steroidogenesis pathway and adversely affect reproduction in fish. , Recent studies have found that cholesterol was significantly decreased in zebrafish after 21 d exposure of 1.0 mg/L boscalid, which may affect the downstream synthesis of steroid hormones in zebrafish. , …”

Section: Introductionmentioning

confidence: 99%

Exposure to Boscalid Induces Reproductive Toxicity of Zebrafish by Gender-Specific Alterations in Steroidogenesis

Qian

Zhang

et al. 2020

Environ. Sci. Technol.

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Boscalid is a succinate dehydrogenase inhibitor fungicide and is frequently detected in surface water. Due to the frequent detection of boscalid, we evaluated its impact on the reproduction of adult zebrafish following a 21 d exposure to 0, 0.01, 0.1, and 1.0 mg/L. Following exposure to boscalid, the fertility of female zebrafish and fertilization rate of spawning eggs were reduced in a concentration-dependent manner up to a respective 87% and 20% in the highest concentration. A significant 16% reduction in the percentage of late vitellogenic oocytes was noted in ovaries, and a significant 74% reduction in the percentage of spermatids in testis was also observed after treatment with 1.0 mg/L. 17β-Estradiol (E2) concentrations decreased significantly in females (34% decrease) but significantly increased in males (15% increase) following 1.0 mg/L boscalid treatment. The expression of genes (such as era, er2b, cyp19a, and cyp19b) related to the hypothalamus-pituitary-gonad-liver (HPGL) axis was significantly altered and positively correlated with E2 concentrations in female and male zebrafish (p < 0.05). Molecular docking results revealed that the binding modes between boscalid and target proteins (ER and CYP19) of zebrafish were similar to that of the reference compounds and the target proteins. The binding energies indicate that boscalid may have a weak estrogen-like binding effect or CYP19 inhibition, potentially altering the HPGL axis, thereby reducing E2 concentrations and fecundity in females. In contrast, boscalid caused significant induction of E2 steroidogenesis and subsequent feminization of gonads in males, indicating gender-specific adverse outcome pathways.

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“…[ 40 , 41 ]. Reproductive impairment can also be studied by assessing sperm motility, egg depositions, and steroid hormone levels [ 42 , 43 ].…”

Section: Toxicological and Biodistribution Evaluation Of Nanoparticles Using Zebrafish: Focus On Macrophagesmentioning

confidence: 99%

Zebrafish Models for the Safety and Therapeutic Testing of Nanoparticles with a Focus on Macrophages

et al. 2021

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New nanoparticles and biomaterials are increasingly being used in biomedical research for drug delivery, diagnostic applications, or vaccines, and they are also present in numerous commercial products, in the environment and workplaces. Thus, the evaluation of the safety and possible therapeutic application of these nanomaterials has become of foremost importance for the proper progress of nanotechnology. Due to economical and ethical issues, in vitro and in vivo methods are encouraged for the testing of new compounds and/or nanoparticles, however in vivo models are still needed. In this scenario, zebrafish (Danio rerio) has demonstrated potential for toxicological and pharmacological screenings. Zebrafish presents an innate immune system, from early developmental stages, with conserved macrophage phenotypes and functions with respect to humans. This fact, combined with the transparency of zebrafish, the availability of models with fluorescently labelled macrophages, as well as a broad variety of disease models offers great possibilities for the testing of new nanoparticles. Thus, with a particular focus on macrophage–nanoparticle interaction in vivo, here, we review the studies using zebrafish for toxicological and biodistribution testing of nanoparticles, and also the possibilities for their preclinical evaluation in various diseases, including cancer and autoimmune, neuroinflammatory, and infectious diseases.

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Reproductive toxicity of azoxystrobin to adult zebrafish (Danio rerio)

Cited by 102 publications

References 43 publications

Mycotoxin zearalenone induced gonadal impairment and altered gene expression in the hypothalamic–pituitary–gonadal axis of adult female zebrafish (Danio rerio)

Mycotoxin zearalenone induced gonadal impairment and altered gene expression in the hypothalamic–pituitary–gonadal axis of adult female zebrafish (Danio rerio)

Exposure to Boscalid Induces Reproductive Toxicity of Zebrafish by Gender-Specific Alterations in Steroidogenesis

Zebrafish Models for the Safety and Therapeutic Testing of Nanoparticles with a Focus on Macrophages

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