Reproductive Proteomics Comes of Age

Karr, Timothy L.

doi:10.1074/mcp.e119.001418

Cited by 9 publications

(5 citation statements)

References 29 publications

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“…Divergence in protein identity or abundance between taxa could disrupt ejaculate × female reproductive tract interactions, leading to PMPZ isolation (Goenaga et al., 2015; Nakadera et al., 2020). High‐throughput proteomics using liquid chromatography tandem mass spectrometry (LC‐MS/MS) has revolutionized identification and quantification of Sfps, revealing that the male ejaculate often contains hundreds of unique proteins (Bayram et al., 2019; Karr, 2019; McDonough et al., 2016; Rowe et al., 2019; Whittington et al., 2019). Using LC‐MS/MS combined with genomics, Sfps can be predicted by identifying ejaculate proteins with a signal peptide sequence, sometimes called the “secretome,” and those secretome proteins that have an extracellular signal sequence, sometimes called the “exoproteome” (Ahmed‐Braimah et al., 2017; Avila et al., 2011; Bayram et al., 2019; Karr et al., 2019; Sepil et al., 2019).…”

Section: Introductionmentioning

confidence: 99%

Seminal fluid protein divergence among populations exhibiting postmating prezygotic reproductive isolation

et al. 2020

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Despite holding a central role in fertilization, reproductive traits often show elevated rates of evolution and diversification. The rapid evolution of seminal fluid proteins (Sfps) within populations is predicted to cause mis‐signalling between the male ejaculate and the female during and after mating resulting in postmating prezygotic (PMPZ) isolation between populations. Crosses between Drosophila montana populations show PMPZ isolation in the form of reduced fertilization success in both noncompetitive and competitive contexts. Here we test whether male ejaculate proteins produced in the accessory glands or ejaculatory bulb differ between populations using liquid chromatography tandem mass spectrometry. We find more than 150 differentially abundant proteins between populations that may contribute to PMPZ isolation, including a number of proteases, peptidases and several orthologues of Drosophila melanogaster Sfps known to mediate fertilization success. Males from the population that elicit the stronger PMPZ isolation after mating with foreign females typically produced greater quantities of Sfps. The accessory glands and ejaculatory bulb show enrichment for different gene ontology (GO) terms and the ejaculatory bulb contributes more differentially abundant proteins. Proteins with a predicted secretory signal evolve faster than nonsecretory proteins. Finally, we take advantage of quantitative proteomics data for three Drosophila species to determine shared and unique GO enrichments of Sfps between taxa and which potentially mediate PMPZ isolation. Our study provides the first high‐throughput quantitative proteomic evidence showing divergence of reproductive proteins between populations that exhibit PMPZ isolation.

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Section: Introductionmentioning

confidence: 99%

Seminal fluid protein divergence among populations exhibiting postmating prezygotic reproductive isolation

et al. 2020

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“…This knowledge aids in understanding the regulatory networks that govern fertility and reproductive performance. Proteomics research has enhanced our understanding of the proteins involved in chicken reproduction and the intricate protein interactions that govern reproductive activities via the study of the entire set of proteins found in reproductive organs or cells [ 3 , 11 ]. Proteomic techniques have found biomarkers associated with fertility, egg quality, and sperm function, allowing for the development of diagnostic tools and breeding strategies [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

The Omics Revolution in Understanding Chicken Reproduction: A Comprehensive Review

Wadood,

Zhang

2024

CIMB

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Omics approaches have significantly contributed to our understanding of several aspects of chicken reproduction. This review paper gives an overview of the use of omics technologies such as genomics, transcriptomics, proteomics, and metabolomics to elucidate the mechanisms of chicken reproduction. Genomics has transformed the study of chicken reproduction by allowing the examination of the full genetic makeup of chickens, resulting in the discovery of genes associated with reproductive features and disorders. Transcriptomics has provided insights into the gene expression patterns and regulatory mechanisms involved in reproductive processes, allowing for a better knowledge of developmental stages and hormone regulation. Furthermore, proteomics has made it easier to identify and quantify the proteins involved in reproductive physiology to better understand the molecular mechanisms driving fertility, embryonic development, and egg quality. Metabolomics has emerged as a useful technique for understanding the metabolic pathways and biomarkers linked to reproductive performance, providing vital insights for enhancing breeding tactics and reproductive health. The integration of omics data has resulted in the identification of critical molecular pathways and biomarkers linked with chicken reproductive features, providing the opportunity for targeted genetic selection and improved reproductive management approaches. Furthermore, omics technologies have helped to create biomarkers for fertility and embryonic viability, providing the poultry sector with tools for effective breeding and reproductive health management. Finally, omics technologies have greatly improved our understanding of chicken reproduction by revealing the molecular complexities that underpin reproductive processes.

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“…Accordingly, in the current study we re-interrogated the Drosophila melanogaster sperm proteome using direct solubilization of sperm followed by on-line fractionation of tryptic peptides and report on a significant increase in both proteome size and content. D. melanogaster with its excellent genome annotation provides a powerful genetic and functional genomics model system to understand reproduction and fertility (e.g., (13)). Our previous efforts identified over 1,000 D. melanogaster sperm proteins with prior versions designated DmSP1 (7) and DmSP2 (14).…”

Section: Introductionmentioning

confidence: 99%

“…D. melanogaster with its excellent genome annotation provides a powerful genetic and functional genomics model system to understand reproduction and fertility (e.g., (13)). Our previous efforts identified over 1,000 D. melanogaster sperm proteins with prior versions designated DmSP1 (7) and DmSP2 (14).…”

Section: Introductionmentioning

confidence: 99%

Functional diversity and evolution of the Drosophila sperm proteome

Garlovsky

Sandler

Karr

2022

Preprint

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Given the central role fertilization plays in the health and fitness of sexually reproducing organisms and the well-known evolutionary consequences of sexual selection and sperm competition, knowledge gained by a deeper understanding of sperm (and associated reproductive tissues) proteomes has proven critical to the field's advancement. Due to their extraordinary complexity, proteome depth-of-coverage is dependent on advancements in technology and related bioinformatics, both of which have made significant advancements in the decade since the last Drosophila sperm proteome was published. Here we provide an updated version of the Drosophila melanogaster sperm proteome (DmSP3) using improved separation and detection methods and an updated genome annotation. We identified 2563 proteins, with label-free quantitation (LFQ) for 2125 proteins. Combined with previous versions of the sperm proteome, the DmSP3 contains a total of 3176 proteins. The top 20 most abundant proteins contained the structural elements alpha- and beta-tubulins and sperm leucyl-aminopeptidases (S-Laps). Both gene content and protein abundance were significantly reduced on the X chromosome, a finding consistent with prior genomic studies of the X chromosome gene content and evolution. This study identified 9 of the 16 Y-linked proteins, including known testis-specific male fertility factors. LFQ measured significant levels for 75/83 ribosomal proteins (RPs) we identified, including a number of core constituents. The role of this unique subset of RPs in sperm is unknown. Surprisingly, our expanded sperm proteome also identified 122 seminal fluid proteins (Sfps), proteins found predominantly in the accessory glands. The possibility of tissue contamination from seminal vesicle or other reproductive tissues was addressed using concentrated salt and detergent treatments. Salt treatment had little effect on sperm proteome composition suggesting only minor contamination during sperm isolation while a significant fraction of Sfps remained associated with sperm following detergent treatment suggesting Sfps may arise within, and have additional functions, in sperm per se.

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Reproductive Proteomics Comes of Age

Cited by 9 publications

References 29 publications

Seminal fluid protein divergence among populations exhibiting postmating prezygotic reproductive isolation

Seminal fluid protein divergence among populations exhibiting postmating prezygotic reproductive isolation

The Omics Revolution in Understanding Chicken Reproduction: A Comprehensive Review

Functional diversity and evolution of the Drosophila sperm proteome

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