Reproductive properties of diatoms significant for their cultivation and biotechnology

Давидович, Н. А.; Давидович, О. И.; Podunai, Yu. A.; Shorenko, K. I.; Куликовский, М. С.

doi:10.1134/s1021443715020041

Cited by 15 publications

(15 citation statements)

References 48 publications

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“…The fusiform and triradiate morphotypes have previously been described to be poorly silicified whereas the oval morphotype is sensitive to the presence of silicic acid as the oval cells contain organized silicified frustules 20 . In addition, all diatoms reproduce by vegetative division, thus leading to a reduction of the average size of the population 21 , 22 . Indeed, during the vegetative division, one daughter cell (the cell that inherits the hypotheca of the parent cell) is smaller than the mother cell 22 .…”

Section: Introductionmentioning

confidence: 99%

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Comparative in depth RNA sequencing of P. tricornutum’s morphotypes reveals specific features of the oval morphotype

Ovide

Kiefer‐Meyer

Bérard

et al. 2018

Sci Rep

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Phaeodactylum tricornutum is the most studied diatom encountered principally in coastal unstable environments. It has been hypothesized that the great adaptability of P. tricornutum is probably due to its pleomorphism. Indeed, P. tricornutum is an atypical diatom since it can display three morphotypes: fusiform, triradiate and oval. Currently, little information is available regarding the physiological significance of this morphogenesis. In this study, we adapted P. tricornutum Pt3 strain to obtain algal culture particularly enriched in one dominant morphotype: fusiform, triradiate or oval. These cultures were used to run high-throughput RNA-Sequencing. The whole mRNA transcriptome of each morphotype was determined. Pairwise comparisons highlighted biological processes and molecular functions which are up- and down-regulated. Finally, intersection analysis allowed us to identify the specific features from the oval morphotype which is of particular interest as it is often described to be more resistant to stresses. This study represent the first transcriptome wide characterization of the three morphotypes from P. tricornutum performed on cultures specifically enriched issued from the same Pt3 strain. This work represents an important step for the understanding of the morphogenesis in P. tricornutum and highlights the particular features of the oval morphotype.

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Section: Introductionmentioning

confidence: 99%

“…Therefore, auxosporulation seems less crucial for the long term survival of the P . tricornutum population 21 , 26 , 27 . It is possible that this difference in the reproduction process is correlated with the slightly silicified cell wall of P .…”

Section: Introductionmentioning

confidence: 99%

Comparative in depth RNA sequencing of P. tricornutum’s morphotypes reveals specific features of the oval morphotype

Ovide

Kiefer‐Meyer

Bérard

et al. 2018

Sci Rep

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“…In centric diatoms, genetic sex determination is absent as is evident from their homothallic mating system, with male and female gametangia originating from the same clonal population (Davidovich et al 2015). Since environmental cues are major triggers for the induction of sexual reproduction in centrics, environmental sex-determining systems are possibly at play to determine the male or female development of gametangia.…”

Section: Sex Determination In Diatomsmentioning

confidence: 99%

“…In this chapter we outline the main features of the diatom life cycle stages, focusing on recent contributions by molecular approaches. Readers interested in the general biology of diatom life cycles can refer to various review papers (Chepurnov et al 2004;Davidovich et al 2015;Mann 2011;Montresor et al 2016;Poulíčková et al 2019) and a recent work proposing a unified terminology for diatom life cycle stages (Kaczmarska et al 2013).…”

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confidence: 99%

The Molecular Life of Diatoms

2022

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Diatom life cycles are unusual among microalgae by being diplontic with a long diploid vegetative phase and a short-lived haploid phase (gametes). Life cycle progression in diatoms is controlled by the cell size reduction-restitution cycle and is intimately linked to their peculiar mode of cell division and siliceous cell wall. Sexual reproduction is primarily cell-size dependent although environmental cues may be needed to trigger gametogenesis in centric diatoms. Although population genetic data suggest sexual reproduction to occur in most species and meiotic genes are widely conserved among diatoms, sexual events are seldom observed in nature. Recent laboratory studies have started to unveil complex pheromone signaling cascades during sexual reproduction in pennate diatoms. Likewise, significant progress has been made in the identification of mating type determination mechanisms in heterothallic species, where several conserved, but as yet functionally uncharacterized, genes involved in sexual reproduction have been identified. While many aspects of diatom life cycle regulation remain to be

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“…First, micro-algae evolve rapidly, resulting in genomic changes during long-term maintenance in culture 18 , 19 . Secondly, the peculiar diatom life cycle, during which consecutive mitotic divisions lead to a gradual cell size reduction in most diatom species 20 , leads to changes in physiology 21 and ultimately in loss of diatom culture viability 22 . It is therefore essential that long-term preservation is achieved in a culture-independent manner, either through cryopreservation or by inducing resting stages.…”

Section: Introductionmentioning

confidence: 99%

Expanding the toolbox for cryopreservation of marine and freshwater diatoms

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Pinseel

Decker

et al. 2018

Sci Rep

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Diatoms constitute the most diverse group of microalgae and have long been recognised for their large biotechnological potential. In the wake of growing research interest in new model species and development of commercial applications, there is a pressing need for long-term preservation of diatom strains. While cryopreservation using dimethylsulfoxide (DMSO) as a cryoprotective agent is the preferred method for long-term strain preservation, many diatom species cannot be successfully cryopreserved using DMSO. Therefore, in this study, we studied cryopreservation success in six different diatom species, representing the major morphological and ecological diatom groups, using a range of DMSO concentrations and Plant Vitrification Solution 2 (PVS2) as an alternative cryoprotectant to DMSO. In addition, we tested whether suppressing bacterial growth by antibiotics accelerates the post-thaw recovery process. Our results show that the effects of cryoprotectant choice, its concentration and the addition of antibiotics are highly species specific. In addition, we showed that PVS2 and antibiotics are useful agents to optimize cryopreservation of algae that cannot survive the traditional cryopreservation protocol using DMSO. We conclude that a species-specific approach will remain necessary to develop protocols for diatom cryopreservation and to increase their representation in public culture collections.

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Reproductive properties of diatoms significant for their cultivation and biotechnology

Cited by 15 publications

References 48 publications

Comparative in depth RNA sequencing of P. tricornutum’s morphotypes reveals specific features of the oval morphotype

Comparative in depth RNA sequencing of P. tricornutum’s morphotypes reveals specific features of the oval morphotype

The Molecular Life of Diatoms

Expanding the toolbox for cryopreservation of marine and freshwater diatoms

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