The objectives of this research were to determine the effects of high inclusion of canola meal (CM) in gestation and lactation diets on lactation performance, milk composition, energy and nutrient digestibility, and gut health in sows and suckling piglet performance. Standardized ileal digestibility (SID) of CP and AA and standardized total tract digestibility (STTD) of P in CM fed to gestating and lactating sows with or without a multi-enzyme complex (MC) was also determined. In Study 1, results showed no differences in sow and litter performance when soybean meal was replaced with increasing levels of CM in lactation diets. However, energy and nutrient digestibilities were reduced with increasing dietary CM inclusion, probably due to increased fiber content. Results indicated that inclusion of up to 30% CM in lactation diet could support satisfactory sow and suckling piglet performance. Results from Study 2 indicated that up to 30% CM could be included in sow diets as a sole protein source from early gestation until weaning without affecting sow and suckling piglet performance, energy and nutrient digestibility, along with an increase in the abundance of beneficial bacteria in the gut. Furthermore, enzyme supplementation improved P digestibility post-farrowing in sows fed CM-containing diets. From