Reproductive parameters of male crab-eating foxes (Cerdocyon thous) subjected to pharmacological semen collection by urethral catheterization

Silva, Maitê Cardoso Coelho da; Jorge‐Neto, Pedro Nacib; Miranda, Giovana Martins; Csermak‐Jr, Antônio Carlos; Zanella, Ricardo; Pizzutto, Cristiane Schilbach; Colbachini, Helen; Silva, Alexandre Rodrigues; Araújo, Gediendson Ribeiro de; Deco‐Souza, Thyara de

doi:10.1016/j.therwi.2022.100004

Cited by 6 publications

(9 citation statements)

References 52 publications

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“…Currently, pharmacological semen collection by urethral catheterization is the gold-standard method for obtaining semen in wild felines ( Araujo et al, 2018 , 2020b ; Jeong et al, 2018 ; Jorge et al, 2019 ; Kheirkhah et al, 2017 ; Lueders et al, 2012 ), other wild species ( Araujo et al, 2023 ; Curry and Roth, 2016 ; Jeong et al, 2019 ; Silinski et al, 2002 ; Silva et al, 2022 ) and in domestic species ( Cavalero et al, 2019 ; Madrigal-Valverde et al, 2021 ; Silva et al, 2021 ), and it allowed efficient semen recovery from the five male jaguars in this study. The first step in the development of assisted reproduction technology for a species is the effective collection of sperm, which aims to permit, in the future, the growth of genetic variety across in situ and ex situ populations, embracing the concept of One Conservation ( Pizzutto et al, 2021 ).…”

Section: Discussionmentioning

confidence: 83%

“…The testicular volume was measured using a caliper and applying the equation for an ellipsoid 4/3 × π × L/2 × B1/2 × B2/2, where L, B1, and B2 are the length and two breadths of the ellipsoid ( Harcourt et al, 1995 ), then subsequently converted to grams (1.046) ( Johnson et al, 1981 ). The relative testicular weight (RTW) was determined by dividing the total testes' weight by the body weight ( Jorge-Neto et al, 2020c ; Silva et al, 2022 ; Silvatti et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

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Cryopreservation of jaguar (Panthera onca) sperm cells using different cryoprotectants and different thawing temperatures

et al. 2023

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The cryopreservation of jaguar semen must be improved to produce high-quality biobanking doses. Until now, the rare studies of semen freezing in the species have only evaluated glycerol, always with a significant reduction in sperm quality in thawed semen. The purpose of this study was to assess the efficacy of three cryoprotectants, dimethylsulfoxide (DMSO), glycerol (GLY), and methanol (MET), in the cryopreservation of jaguar semen in an LDL-based extender, as well as the effect of thawing temperature on dosage quality. Five mature males with a history of reproduction were used. On the males, an infrared thermal image (IRT) was captured, the spicules and testes were analyzed, and the CASA system was used to evaluate the quality of fresh and thawed sperm. The superficial IRT was 4.6 ± 1.2 °C cooler than the anal sphincter, and the semen measured between 27.3 and 28.7 °C shortly after exiting the urethra. The total motility of fresh sperm was 55.3 ± 22.6%, and progressive motility was 36.3 ± 18%. The total motility of thawed sperm was 5.28 ± 2.51%, 4.49 ± %2.49, and 0.51 ± 0.62% for DMSO, GLY, and MET, respectively. DMSO and GLY performed better than MET, and there was no difference in thawing temperature (37°C 30 s vs. 50°C 12 s). All animals exhibit a considerable level of morphological changes in sperm. Low amounts of total and progressive motility were found in the thawed sperm. Males with a high level of sperm morphological changes were found to be fertile, but the lone male with normospermia was infertile. Thus, we contest the applicability of the commonly used morphological classification for bovines to felid species.

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Section: Discussionmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Cryopreservation of jaguar (Panthera onca) sperm cells using different cryoprotectants and different thawing temperatures

et al. 2023

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Section: Reproductive Characteristicsmentioning

confidence: 99%

“…There are only a few reports of semen collection by electroejaculation in Maned wolf ( 110 , 111 ) or urethral catheterization in Maned wolf and crab-eating fox ( 112 , 113 ). Collection of seminal samples by urethral catheterization of males pre-treated with a medetomidine and ketamine combination seems to be a promising technique for obtaining seminal samples from wild canids in situ ( 112 , 113 ). However, all attempts to collect semen from Maned wolf and Crab-eating fox by electroejaculation or urethral catheterization performed in our laboratory were unsuccessful and/or samples were contaminated with urine (unpublished data).…”

Section: Reproductive Characteristicsmentioning

confidence: 99%

Reproduction in South American wild canids—A review

et al. 2022

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Canids occupy the top of the food chain and are fundamental in sustaining a wild animal/environmental balance. South America, the most biodiverse continent, has 11 species of canids inhabiting diverse biomes, with or without overlapping territories. Although several species are threatened, little is known about their reproductive biology. Remarkably, basic knowledge regarding ejaculate characteristics, sexual behavior, female reproductive cycles, pregnancy and management, and parturition are scarce or absent. These gaps complicate or preclude development of conservation programs. This review compiles the current knowledge of the reproductive biology of South American canids and discusses implications of this scenario.

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“…Semen is retrieved by capillarity using a tomcat-type semi-rigid urethral catheter with an open end. This approach eliminates the need for catheters with side openings and ensures efficient and reliable semen collection ( Zambelli et al, 2008 ; Silva et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Pharmacological semen collection in domestic and wild canids and felids: literature review

Carneiro,

Motheo

2023

Anim. Reprod.

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Semen collection methods vary greatly and rely on the practitioner's expertise, available materials, and the specific behavioral traits of the male animals involved. When it comes to domestic cats, wild felids, and canids, semen collection is particularly challenging. Thus, given the difficulty of semen collection in these species, pharmacological semen collection (PSC) stands out since it is a quick and straightforward method that does not require specific equipment. The PSC consists of administering α2-adrenergic receptor agonist drugs, mainly medetomidine, and dexmedetomidine, aiming semen release into the urethra with posterior urethral catheterization and sperm recovery. This technique was primarily described in domestic cats and wild felids, and despite the decreased seminal volume, the retrieved semen is highly concentrated and presents good quality. However, further studies are required to optimize semen collection in domestic dogs and wild canids. Therefore, the purpose of this review is to provide a comprehensive overview of the research developed on pharmacological semen collection (PSC) in the past few decades. The objective is to equip professionals with the essential knowledge required for the efficient application of this technique in both domestic and wild canids and felids and to make a valuable contribution to conservation efforts and the preservation of biodiversity, aligning with the principles of One Conservation.

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Reproductive parameters of male crab-eating foxes (Cerdocyon thous) subjected to pharmacological semen collection by urethral catheterization

Cited by 6 publications

References 52 publications

Cryopreservation of jaguar (Panthera onca) sperm cells using different cryoprotectants and different thawing temperatures

Cryopreservation of jaguar (Panthera onca) sperm cells using different cryoprotectants and different thawing temperatures

Reproduction in South American wild canids—A review

Pharmacological semen collection in domestic and wild canids and felids: literature review

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