Reproductive organ transplantation: advances and controversies

Bedaiwy, Mohamed A.; Shahin, Ahmed Y.; Falcone, Tommaso

doi:10.1016/j.fertnstert.2008.08.009

Cited by 50 publications

(46 citation statements)

References 188 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Orthotopic ovarian transplantation is a powerful technique that can be put into use for maintaining lines of transgenic mice 9 and is an active area of research aimed to preserve the fertility of young women diagnosed with cancer 10 . Similarly, orthotopic transplantation of cryopreserved ovarian tissue would facilitate efforts aimed to preserve the avian germ plasm.…”

Section: Discussionmentioning

confidence: 99%

Overektomi Operasyonu Zamanının Kuluçkadan Yeni Çıkmış Civcivlerin Hayatta Kalma Oranına Etkileri

Karagenc¹,

Türkyilmaz²,

Sandıkçı³

2009

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

SummaryPreliminary experiments performed in our laboratory on orthotopic transplantation of the ovarian tissue in newly-hatched chicks demonstrated that majority of chicks could not survive the surgical technique described by Song and Silversides (2006) and die within 24 h following the operation. A series of experiments were performed in the present study to determine underlying causes of this high mortality rate. A total of 50 newly-hatched chicks (day 0) went through the operation in the first series of experiments. It was determined that the survival rate did not exceed 36% and majority of chicks died within 24 h following the operation in agreement with our preliminary observations. The effect of the removal of the yolk sac on various time points in reference to the day of hatching was examined in a second series of experiments. When the yolk sac was removed on the day of hatching (n=10, day 0), only 30% of the chicks survived the operation. When the yolk sac is removed either on day1 (n=11, 24 h post-hatch) or on day2 (n=8, 48 h post-hatch) 73 and 100% of chicks survived the operation, respectively. These results suggest that the orthotopic transplantation of the ovarian tissue in the chick should be performed one or two days following the day of hatching to avoid the high mortality rate associated with the surgical technique. Keywords: Chick, Orthotopic, Ovariectomy, Ovary, Transplantation Overektomi Operasyonu Zamanının Kuluçkadan Yeni Çıkmış Civcivlerin Hayatta Kalma Oranına Etkileri ÖzetBirimimizde kuluçkadan yeni çıkmış olan civcivlerde ortotopik ovaryum transplantasyonu üzerine yapmış olduğumuz ön çalışmalar, Song ve Silversides (2006) tarafından tanımlanan operasyon tekniğinin uygulanması sonucunda operasyon yapılan civcivlerin çoğunluğunun operasyonu izleyen 24 saat içerisinde öldüğünü göstermiştir. Sunulan çalışmada, söz konusu tekniğin uygulanmasına bağlı olarak ortaya çıkan bu yüksek ölüm oranının nedenlerini belirlemek amacıyla bir seri deney gerçekleştirilmiştir. Birinci seri deneylerde toplam 50 adet kuluçkadan yeni çıkmış civcive (0. gün) overektomi operasyonu uygulanmıştır. Elde edilen veriler, yaşama oranının %36'yı geçmediğini ve ön verilerimizi doğrular nitelikte civcivlerin çoğunluğunun operasyonu takiben 24 saat içerisinde öldüğünü göstermiştir. İkinci seri deneylerde, kuluçkadan çıkış günü referans alınarak yumurta sarı kesesinin uzaklaştırılmasının ölüm oranları üzerine olan etkisi incelenmiştir. Yumurta sarı kesesinin kuluçkadan çıkış günü (n=10, 0. gün) uzaklaştırılması durumunda, civcivlerin yalnızca %30'u hayatta kalmıştır. Yumurta sarı kesesinin kuluçkadan çıkışı izleyen 24. saatte (n=11, 1. gün) ya da 48. saatte (n=8, 2. gün) uzaklaştırılması durumunda ise, civcivlerin sırasıyla %70 ve %100'ü hayatta kalmıştır. Bu bulgular, operasyon tekniğine bağlı olarak gelişen yüksek ölüm oranlarını azaltmak amacıyla, civcivlerde ovaryum transplantasyon işleminin kuluçkadan çıkışı takip eden 24. ya da 48. saatlerde uygulanması gerektiğini göstermektedir.

show abstract

Section: Discussionmentioning

confidence: 99%

Overektomi Operasyonu Zamanının Kuluçkadan Yeni Çıkmış Civcivlerin Hayatta Kalma Oranına Etkileri

Karagenc¹,

Türkyilmaz²,

Sandıkçı³

2009

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

show abstract

“…Дослідження толерантності яєчників до ішемії, критичний час, а також вплив різних фізико-хімічних факторів на структурно-функціональні пошкодження оваріальної тканини є актуальним завданням у сучасній репродуктивній фізіології [14]. Це зумовлено тим, що трансплантація тканини яєчників є однією з основних стратегій збереження репродуктивної та стероїдогеної функції у жінок після курсів хіміо-та радіотерапії [15].…”

Section: вступunclassified

Dynamics of structural change of ovarian tissue under ischemia

Bozhkova¹,

Kiroshka²,

Havas³

et al. 2014

Fiziol Zh

View full text Add to dashboard Cite

“…Another receptor site commonly used for xenotransplantation is the kidney capsule (Gosden et al 1994), which is chosen due to the higher vascularity of the kidneys compared to that of other sites such as under the skin or in the ovarian pouch (Yang et al 2006). To perform this technique, either immunosuppressive drugs (Samaniego et al 2006) or immunodeficient animals (Aubard 2003) should be used in order to reduce the possibility of rejection of the transplanted tissue (Bedaiwy et al 2008, Silber et al 2010, Silber 2012, Von Schonfeldt et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts

et al. 2017

View full text Add to dashboard Cite

RESUMO.-[Respostas bioquímicas, termográficas e foliculares de modelos murinos tratados hormonalmente após xenotransplante de ovário bovino sob a cápsula renal.] Este estudo teve como objetivo avaliar as características dos dois diferentes modelos de murinas tratadas hormonalmente após xenotransplante de tecido ovariano bovino sob a cápsula renal. Dois modelos de camundongos imunodeficientes (BALB/c NUDE e C57BL6 SCID) receberam fragmentos de ovário de novilhas e cada grupo foi submetido a dois tratamentos hormonais de eCG ou uma combinação de FSH+LH. This study aimed to evaluate the characteristics of two different murine models of hormone-treated renal-encapsulated bovine ovarian tissue xenotransplantation. Two immunodeficient mouse models (BALB/c Nude and C57BL6 SCID) were xenografted with ovarian pieces from heifers and each group was subjected to two hormonal treatments of eCG or a combination of FSH+LH. Donor ovaries and recipients were evaluated by histology and infrared thermography at different times. At the time of xenograft collection, animals were evaluated for alterations in hepatorenal biochemistry. The statistical test used in the study was ANOVA, followed by Tukey's test. Among the strains, 80% of C57BL6 SCID and 77% of BALB/c Nude mice showed development and vascularization of the transplanted tissue, which acquired cyclicity at 19 and 9 days post-transplant, respectively. Hemorrhagic follicles in xenografts induced with FSH+LH were found in the C57BL6 SCID strain. Infrared thermography was insufficient to distinguish the tissue donor recipient. In conclusion, the C57BL6 SCID strain appears to be the best host for ovarian xenografts, since the transplants in these mice were viable and showed robust follicular development. This work will aid future choices of immunodeficient strains for xenografting procedures.

show abstract

Reproductive organ transplantation: advances and controversies

Cited by 50 publications

References 188 publications

Overektomi Operasyonu Zamanının Kuluçkadan Yeni Çıkmış Civcivlerin Hayatta Kalma Oranına Etkileri

Overektomi Operasyonu Zamanının Kuluçkadan Yeni Çıkmış Civcivlerin Hayatta Kalma Oranına Etkileri

Dynamics of structural change of ovarian tissue under ischemia

Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts

Contact Info

Product

Resources

About