Reproductive impairment of sea urchin upon chronic exposure to cadmium. Part II: Effects on sperm development

Au, Doris W.T.; Reunov, A. A.; Wu, Rongben

doi:10.1016/s0269-7491(00)00036-1

Cited by 49 publications

(38 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present results indicated that the samples of RN exhibited more differentiation and development of the flagellum than that of LM samples. The deformities change of the sperm's flagellum of O. niloticus of LM may lead to decreasing the capacity of sperm motility and reducing its fertilization capacity, and consequently lead to failure of reproduction [50,51]. Most probably these alterations in sperms may result from environmental hazards increasing the levels of Cd and Pb.…”

Section: Discussionmentioning

confidence: 99%

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Shalaby¹,

Migeed²

2012

J Environ Anal Toxicol

View full text Add to dashboard Cite

IntroductionThe importance of Nile tilapia, Oreochromis niloticus in aqua cultures is due to its spreading in most countries of the world [1]. It breads in captivity and wide variety of water conditions [2]. Lake Manzalah (LM) is one of the most important lakes in Egypt that provides more than 70% of the total fishery of the country [3] and considered as a highly polluted with toxic heavy metals and pesticides due to progressive increase of industrial and agricultural drainage, as well as sewage out fall from the surrounding governorates [4]. The mean values of some heavy metals (Cd, Pb, Fe, Cr and Mn) recorded in LM were higher than the world permissible levels [5] and according to the Egyptian laws [6]. Such heavy metals are not lethal to fish, but concentrated in their flesh and create a hazard to the consumer [7,8]. Cd, Pb, Zn, and Cr are considered the main causes of pollution in aquatic ecosystem [9]. The serious effect of these metals is derived from their persistence, toxicity and bioaccumulation, and consequently exert dangerous problem to man [10]. Experimental studies on heavy metals proved that they could impair the respiratory functions of the gills; consequently death in fish is probably caused by tissue hypoxia [11]. Metals affect not only the fish morphology, but also all biological activities [12]. Among the heavy metals; Cd and Pb are the serious heavy metals that produce histopathological alterations including liver damage [13], respiratory dysfunctions [14], testicular and ovarian alterations [15][16][17]. These pathological alterations were primarily attributed to damage of cell membranes which allows higher uptake of Cd and Pb and thus the injury extends to more critical targets [18]. Many studies focused on bioaccumulation of heavy metals in fish and biological studies. Thus we aimed in this work to study the testicular histopathological alterations of Oreochromis niloticus using light microscope and the mature sperm using TEM. Materials and Methods Samples collectionSamples of water and 48 specimen of Nile tilapia, Oreochromis niloticus about 14-20 cm in length and 120-200 g in weight were collected from two areas; Demitta branch of RN at Mansoura city as a control site and LM as a polluted area during the spawning season from March-June and transported to the laboratory in tanks provided with oxygen pump. The fish were weighed, dissected and the testes were Abstract Background: Pollution of the aquatic environment by inorganic and organic chemicals is a major factor posing serious threat to the survival of aquatic organisms including fish. Lake Manzala (LM) is one of the Egypt's northern Delta Lakes situated on the Mediterranean Coast of the Delta. It is affected by drainage water polluted by different heavy metals that their concentrations exceed the maximum world permissible levels.

show abstract

Section: Discussionmentioning

confidence: 99%

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Shalaby¹,

Migeed²

2012

J Environ Anal Toxicol

View full text Add to dashboard Cite

show abstract

“…In sperm of the mussel Mytilus edulis treated with 3.3 mM Cu 2+ Ca 2+ levels significantly decreased in mitochondria and acrosomes indicating an increase in the ionic permeability of organelle membranes and possibly resulting in an increase in cytosolic Ca 2+ (Earnshaw et al,1986). Ca 2+ channels on the sperm plasma membrane have also been proposed to be affected after paternal exposure of sea urchins to the heavy metal Cd 2+ (Au et al, 2001 (Nielsen et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Disturbance of Ca 2+ channels and homeostasis by heavy metals have previously been suggested to affect acrosome reaction and motility of invertebrate sperm (Earnshaw et al, 1986, Au et al, 2001. Since sperm motility is directly correlated with fertilization success (Au et al, 2002) further studies are needed to investigate the effects of Cu 2+ and TBP on sperm motility of sea urchins.…”

Section: +mentioning

confidence: 99%

Measuring Ca2+-signalling at fertilization in the sea urchin Psammechinus miliaris: Alterations of this Ca2+-signal by copper and 2,4,6-tribromophenol

Schäfer

Bickmeyer

Köehler

2009

Comparative Biochemistry and Physiology Part C: Toxicology &

View full text Add to dashboard Cite

During fertilization, eggs undergo a temporary rise in the intracellular concentration of free Ca 2+ ions. Using the membrane permeable acetoxymethylester of the fluorescent calcium indicator dye Fura-2, Fura-2 AM, the Ca 2+ -signal at fertilization was not detectable in eggs of the sea urchin Psammechinus miliaris. However, after treatment of the eggs with Fura-2 AM in combination with MK571, an inhibitor for multidrug resistance associated proteins, clear Ca 2+-signals at fertilization could be measured without microinjection of the dye. We used this methodology to detect possible alterations of Ca 2+ -signalling at fertilization by exposure of eggs to environmental pollutants. For this purpose, the heavy metal copper, the bromophenol 2,4,6-tribromophenol, the organic compound bisphenol A and the polycyclic aromatic hydrocarbon phenanthrene were tested for their potential to inhibit fertilization success of P. miliaris. Copper and 2,4,6-tribromophenol showed a dose-dependent effect on fertilization rates of P. miliaris and significantly inhibited fertilization at 6.3 µM Cu 2+ and 1 µM 2,4,6-tribromphenol. Bisphenol A significantly inhibited fertilization success at 438 µM while phenanthrene had no effect up to 56 µM. 6.3 µM copper and 100 µM 2,4,6-tribromophenol significantly increased the Ca 2+ -signal at fertilization. This alteration may contribute to the reduced fertilization rates of P. miliaris after exposure to copper and 2,4,6-tribromophenol.

show abstract

“…Carcinogenic effects, as well as damage of several organs (2)(3)(4)(5), are caused by the exposure to this metal, which also alters gametogenesis and embryogenesis (6,7), interfering with normal development and/or inducing apoptotic responses in embryos (8)(9)(10). A prominent effect of cadmium is the induction of oxidative stress both in vivo and in cultured cells (11)(12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

The effect of cadmium on brain cells in culture

Gerspacher

Scheuber²,

Schiera³

et al. 2009

Int J Mol Med

View full text Add to dashboard Cite

Abstract. Cadmium is a long-living heavy metal, abundantly present in the environment, which accumulates in the body. In this study, we investigated the effects of cadmium on the expression of molecular chaperones, and of certain cell-specific proteins, in a variety of brain cell types in culture, namely primary cultures of rat cortical neurons and astrocytes, a brain capillary endothelial cell line (RB4E.B cells), and pheochromocytoma cells (PC12), induced or not to differentiate by NGF treatment. The metal induces a dose-dependent increase of Hsp70 in all cell types. Responses to the metal are cellspecific in the case of Hsc70 and Hsp90: i) in astrocytes, as well as in PC12 cells, cadmium has no significant effect; ii) in endothelial cells, an increase of both proteins is clearly observable from 20 μM cadmium; iii) both Hsp90 and Hsc70 decrease in neurons treated with high doses of cadmium. Damage to the cytoskeleton in treated cells was also evident. Finally, we report that the metal at high doses induces a decrease of PIPPin (also known as CSD-C2), a putative RNAbinding protein highly expressed in neurons and probably involved in the regulation of histone replacement variant expression.

show abstract

Reproductive impairment of sea urchin upon chronic exposure to cadmium. Part II: Effects on sperm development

Cited by 49 publications

References 33 publications

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Impact of Environmental Contaminants on the Testes of Oreochromis niloticus with Special Reference to Ultrastructure of Spermatozoa in Lake Manzala (Egypt)

Measuring Ca2+-signalling at fertilization in the sea urchin Psammechinus miliaris: Alterations of this Ca2+-signal by copper and 2,4,6-tribromophenol

The effect of cadmium on brain cells in culture

Contact Info

Product

Resources

About