Reproductive impairment in Japanese quail (Coturnix japonica) after in ovo exposure to o,p'-DDT

Halldin, Krister; Holm, Lena; Ridderstråle, Yvonne; Brunström, Björn

doi:10.1007/s00204-002-0417-8

Cited by 53 publications

(37 citation statements)

References 28 publications

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“…Consequently, the permanent effects of the estrogen like compounds on sexual behavioral can be induced by administering during a sensitive developmental stage, such as the period of differentiation or maturation of the reproductive organs (Panzica et al, 2009). Whereas estrogen-induced morphological changes in the testis have been reported to disappear after hatching (Halldin et al, 1999(Halldin et al, , 2003 while the estrogen-induced effects on the male brain are rather permanent. Generally, it can be suggested that effects of high doses of BPA in pubertal males is expected to be irreversible when the pollutant chemical is existent elongated enough during the period of instigation of sexual behavior (Panzica et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Testicular Functions and Sexual Behavior in Male Japanese Quail after Exposure to Bisphenol A

Hanafy¹,

Khalil²,

Abdel-Rahi³

et al. 2015

Asian J. of Poultry Science

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Recently, with the world progress of industries, several adverse factors such as Endocrine Disrupting Chemicals (EDCs) have appeared. These contaminants have negative devastating effects on the reproductive performance in a large number of domestic and wildlife animal species. Therefore, the objective of this study was to investigate the reproductive and physiological changes due to pre-pubertal exposure of Japanese quail males to Bisphenol A (BPA) as one of EDCs (BPA is a synthetic chemical acts as estrogenic effect). Three weeks old male quails were weekly injected intra-peritoneally (at 3, 4 and 5 weeks old) with BPA at doses of 0, 1, 5, or 10 mg kgG 1 b.wt. After 6 weeks exposure of BPA, time of sexual libido, semen characteristics, fertility percent, sexual organs development, histopathology of testes were examined and plasma testosterone concentrations were estimated. The results showed that BPA has adverse and deterioration effects on most of the studied traits. The males received 5 and 10 mg BPA showed significantly delayed time of sexual libido compared with control group. Also, males received 5 mg BPA showed significantly reduced semen volume and cloacal gland area compared with control males. The lowest initial motility and fertility percent (p#0.05) were detected in 10 mg group while the highest values were obtained in the control group. Males treated with 1 and 10 mg BPA had lower (p = 0.028) foam production than those produced in control males. Plasma concentrations of testosterone were significantly reduced (p<0.000) in all treated groups compared with control group. Histologically, the growth of the testes was negatively affected by exposure to/or over 1 mg kgG 1 BPA: Namely, the development of seminiferous tubules and spermatogenesis were severely inhibited compared with control testes. It could be concluded that exposure to estrogenic effects of environmental endocrine disruptors such as BPA before/at puberty lead to malformation of reproductive organs and reduction of reproductive capacity which appears not to regenerate in adult male quail.

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Section: Discussionmentioning

confidence: 99%

Testicular Functions and Sexual Behavior in Male Japanese Quail after Exposure to Bisphenol A

Hanafy¹,

Khalil²,

Abdel-Rahi³

et al. 2015

Asian J. of Poultry Science

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“…Using highly conserved primers flanking the intron of the gene, PCR amplification and agarose electrophoresis, 47 out of 50 bird species were successfully sexing 17 , whereas the method did not provide complete results and spent considerable cost and time. On the other hand, in ovo exposure of EDCs is the most important route in the birds [1][2][3]11 and avian eggs have been elucidated to be a good test system for evaluating effects of EDCs 5,[12][13][14][18][19][20][21][22] . Maternal transfer of 17 beta-estradiol benzoate (EB) to egg yolk has been demonstrated in Japanese quail, in which female quails received EB in the routs of i.m.…”

Section: Discussionmentioning

confidence: 99%

A New Screening Model Using F1(AWE * WE) Japanese Quail Embryo for Evaluating Sex Reversal Effects

et al. 2004

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F 1 (AWE × WE) strain of Japanese quail (Corturnix japonica) is produced from a mating between male AWE (albino plumage color) and female WE (wild plumage color) strains of Japanese quail. Male and female offspring exhibit wild and albino plumage colors, respectively, ruled by a criss-cross inheritance. F 1 (AWE × WE) eggs received 17 beta-estradiol (E 2 ) or methyltestosterone (MT) at 0, 20, 200, 2000, and 20000 ng/egg just before incubation. At 16 days of incubation, embryos were subjected to a complete necropsy and their gonads were grossly observed and examined histopathologically. Viabilities of the embryos at 16 days of incubation were not significantly different between the control and all E 2 groups and between the control and the MT 20, 200 and 2000 ng groups, whereas viability of the MT 20000 ng group was significantly lower than that of the control group. Grossly, genetic sex confirmed by plumage colors coincided completely with sex phenotype of the gonads in all embryos of the control and treated groups. Histopathologically, E 2 exposure induced a dose-dependent feminization such as ovotestis of the left testis. No abnormalities were detected in any male embryos of all the MT groups. E 2 and MT exposures induced no noticeable changes in the ovaries of any embryos. The present study suggests that the sex reversal test using F 1 (AWE × WE) Japanese quail embryos may be a rapid and cost-effective tool to evaluate screening feminization effects of the estrogenic endocrine disrupters. (J Toxicol Pathol 2004; 17: 245-252)

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“…Elsewhere, Gray et al (1982) observed a decrease in testicular weight. Embryonic exposure to xenoestrogens produced a reduction in the growth of the cloacal gland at sexual maturity in male Japanese quail (Adkins, 1979;Halldin et al, 1999;Halldin et al, 2003). This effect may be induced by activation of ERα since propyl pyrazol triol (PPT) treatment during quail embryo development significantly reduced the cloacal gland area in sexually mature males (Mattsson et al, 2008b).…”

Section: Effects On Histopathologymentioning

confidence: 99%

The effects on steroidogenesis and histopathology of adult male Japanese quails (Coturnix coturnix japonica) testis following pre-pubertal exposure to di(n-butyl) phthalate (DBP)

Bello

Madekurozwa

Groenewald

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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Keywords:Di-(n-butyl) phthalate Leydig cell Steroidogenesis Male Japanese quails Endocrine disruptionIn the present study, we have investigated the effects of 30-day dietary (pre-pubertal) exposure to different doses (0 (control), 1, 10, 50, 200 and 400 mg/kg bodyweight/day) of di(n-butyl) phthalate (DBP) on Leydig cells of adult male Japanese quails by quantifying the transcript levels for P450 side-chain cleavage (p450scc), P450c17 (CYP17), and 3β-and 17β-hydroxysteroid dehydrogenase (hsd) using quantitative (real-time) poly-merase chain reaction (qRT-PCR). In addition, the plasma testosterone levels were analysed using radioimmuno-assay (RIA) and testis was examined for evidence of gross pathology and histopathology. Our data showed that pre-pubertal exposure to DBP produced alterations in testicular architecture as evident by poorly developed or misshaped testis, and altered spermatogenesis due to tubular degeneration and atrophy of seminiferous tubules especially in the high DBP dose (200 and 400 mg/ kg) treated groups. In addition, DBP altered several key en-zymes involved in testicular steroidogenesis pathways in an apparent dose-dependent manner. For example, bi-phasic effects of DBP were observed for P450scc and 3β-hsd mRNA, that were generally increasing at low dose 10 mg/kg, and thereafter, an apparent dose-dependent decrease between 50 and 400 mg/kg. The steroidogenic acute regulatory (StAR) protein was at the lowest detectable limits and therefore not quantifiable. These effects did not parallel the non-significant changes observed for plasma testosterone levels. The present data is consis-tent with previous reports showing that DBP modulates Leydig cell steroidogenesis in several species, with a po-tential negative effect on reproduction in those avian species that are vulnerable to endocrine disrupting chemicals.

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Reproductive impairment in Japanese quail (Coturnix japonica) after in ovo exposure to o,p'-DDT

Cited by 53 publications

References 28 publications

Testicular Functions and Sexual Behavior in Male Japanese Quail after Exposure to Bisphenol A

Testicular Functions and Sexual Behavior in Male Japanese Quail after Exposure to Bisphenol A

A New Screening Model Using F1(AWE * WE) Japanese Quail Embryo for Evaluating Sex Reversal Effects

The effects on steroidogenesis and histopathology of adult male Japanese quails (Coturnix coturnix japonica) testis following pre-pubertal exposure to di(n-butyl) phthalate (DBP)

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